Cell sorting system

Patent metadata

Field	Value
Publication number	US-2021180004-A1
Application number	US-202017123874-A
Country	US
Kind code	A1
Filing date	Dec 16, 2020
Priority date	Dec 16, 2019
Publication date	Jun 17, 2021
Grant date	—

Abstract

Devices and methods for sorting cells are provided that use a holder with cells adherent to a material whose adhesive properties are affected by light, a pixelated light-generating array, and a pixelated photosensitive array. Selected cells can be released from the adhesive layer after illumination of the adhesive layer by the light-generating array.

First claim

1 . An apparatus comprising a holder of cells, wherein cells are adherent to the holder with a material whose adhesive properties are affected by light, a pixelated light-generating array, and a pixelated photosensitive array, and at least one port for removing the contents of the holder.

2 . The apparatus of claim 1 , where the adhesive material is a photo-responsive hydrogel.

3 . The apparatus of claim 2 , wherein the photo-responsive hydrogel is composed of one or more of the following materials
a. Fibrin Alginate b. Polyethylene glycol (PEG)-based hydrogel c. Alginate d. Alginate-lyase e. Gelatin norbornene (GelNB) and PEG f. Gelatin methacryloyl (GelMA) g. Alginate/RGD-alginate h. RGD-alginate i. PEG-GelMA j. Type I collagen- and chitosan-agarose blends k. Fibrin-collagen l. Agarose-collagen m. Methacrylamide gelatin n. Polylactic acid (PLA)/GelMA o. Cellulose and alginate p. Skin-derived ECM q. Polyester urethane urea (PEUU) r. Plasma-alginate s. Albumin t. Chitosan-alginate u. Polystyrene v. Sodium-alginate w. Chelated form of calcium (Ca2+) x. Nitrobenzyl ether derived monomer y. Calcium-chelator such as ethylene diamine tetracetic acid (EDTA) to convert the gel into a solution z. PEG-bis-amine to form a photodegradable cross-linker aa. Fibronectin-derived peptide RGDS to form a photoreleasable tether bb. Photolabile group, ethyl 4-(4-(1-hydroxyethyl)-2-methoxy-5-nitrophenoxy)butanoic acid

4 . The apparatus of claim 1 , where the adhesive material is a polymer film that changes its wettability upon ultraviolet irradiation.

5 . The apparatus of claim 1 , where light increases the adhesive properties of the material.

6 . The apparatus of claim 1 , where light decreases the adhesive properties of the material.

7 . The apparatus of claim 1 , where the light-generating array includes liquid crystal and/or light emitting diodes.

8 . The apparatus of claim 11 , where the photosensitive array includes conventional CCD or CMOS sensors.

9 . The apparatus of claim 1 , where the photosensitive array includes lensless contact imagers.

10 . The apparatus of claim 1 , where the photosensitive array includes very-large-area CCD image sensors.

11 . A method for processing cells, in which selected cells are released from an initially adhesive layer in a chamber after illumination of said adhesive layer from a light-generating array and then transported after release to another chamber.

12 . The method of claim 10 , where the pattern of illumination is selected on the basis of a pattern or patterns formed on a photosensitive layer in or near the chamber.

Assignees

• Weinberg Medical Physics Inc

Inventors

• Weinberg Irving N
• Probst Roland

Classifications

• C12M23/20 Primary

  Material Coatings (immunocoatings C12M25/00) · CPC title

• C12M41/06

  of illumination · CPC title

• C12M31/10

  by light emitting elements located inside the reactor, e.g. LED or OLED · CPC title

• C12M47/04 Primary

  Cell isolation or sorting (purging biological preparations of unwanted cells C12N5/0081, determining the presence or kind of microorganism C12Q1/04) · CPC title

• C12M33/02

  by impregnation, e.g. using swabs or loops (fluid transport using swabs B01L3/5029) · CPC title