Cell culture method and cell culture apparatus

Patent metadata

Field	Value
Publication number	US-2021171904-A1
Application number	US-202117177591-A
Country	US
Kind code	A1
Filing date	Feb 17, 2021
Priority date	Aug 22, 2018
Publication date	Jun 10, 2021
Grant date	—

Abstract

A cell culture method includes culturing a cell in a culture solution that satisfies at least one of condition (I) or condition (II) below.Condition (I): lactic acid concentration in the culture solution being lower than 7.5 mM.Condition (II): ammonia concentration in the culture solution being lower than 2.5 mM.

First claim

What is claimed is:

1 . A cell culture method comprising culturing a cell in a culture solution that satisfies at least one of condition (I) or condition (II) below:
condition (I): lactic acid concentration in the culture solution being lower than 7.5 mM; and condition (II): ammonia concentration in the culture solution being lower than 2.5 mM.

2 . The cell culture method according to claim 1 , wherein the cell is a pluripotent stem cell.

3 . The cell culture method according to claim 1 , wherein the lactic acid concentration in condition (I) is 5 mM or lower.

4 . The cell culture method according to claim 1 , wherein the ammonia concentration in condition (II) is 1 mM or lower.

5 . A cell culture apparatus comprising:
a culture vessel structured to house a cell and a culture solution; and a conditioner structured to condition the culture solution so as to satisfy at least one of condition (I) or condition (II) below: condition (I): lactic acid concentration in the culture solution being lower than 7.5 mM; and condition (II): ammonia concentration in the culture solution being lower than 2.5 mM.

6 . The cell culture apparatus according to claim 5 , wherein the conditioner comprises:
a storage tank structured to store a replenishing solution; a feed pump structured to feed the replenishing solution from the storage tank to the culture vessel; a concentration sensor structured to detect at least one of lactic acid concentration or ammonia concentration in the culture solution; and a controller structured to drive the feed pump in response to result of detection by the concentration sensor.

7 . The cell culture apparatus according to claim 5 , wherein the conditioner comprises:
a substance exchanger structured to house a substance exchange membrane and an ingredient conditioning solution, and, upon feeding of the culture solution, allow substance exchange to proceed through the substance exchange membrane between the ingredient conditioning solution and the culture solution; a circulation pump structured to circulate the culture solution between the culture vessel and the substance exchanger; a concentration sensor structured to detect at least one of lactic acid concentration or ammonia concentration in the culture solution; and a controller structured to drive the circulation pump in response to result of detection by the concentration sensor.

8 . The cell culture apparatus according to claim 5 , wherein the conditioner comprises an adsorbent structured to adsorb at least one of lactic acid or ammonia in the culture solution.

Assignees

• Nikkiso Co Ltd

Inventors

• Kitagawa Fumihiko

Classifications

• C12M29/26

  Conditioning fluids entering or exiting the reaction vessel · CPC title

• C12N2500/60

  Buffer, e.g. pH regulation, osmotic pressure · CPC title

• C12N2500/30

  Organic components · CPC title

• C12N5/0018

  Culture media for cell or tissue culture (media for specific animal cell type C12N5/06) · CPC title

• C12M41/32 Primary

  of substances in solution · CPC title