Devices and methods for laser capture microdissection

Patent metadata

Field	Value
Publication number	US-2021148792-A1
Application number	US-202117162034-A
Country	US
Kind code	A1
Filing date	Jan 29, 2021
Priority date	Aug 10, 2015
Publication date	May 20, 2021
Grant date	—

Abstract

A carrier for holding a biological sample includes a substrate. The substrate is configured to engage a first sample chamber comprising a first opening characterized by a first opening diameter or a second sample chamber comprising a second opening characterized by a second opening diameter that is greater than the first opening diameter. The substrate includes an upper portion, a lower portion, and an intermediate portion disposed between the upper portion and the lower portion. The lower portion is disposed below the upper portion and comprises a bottom surface configured to receive a biological sample. The intermediate portion is characterized by a first substrate diameter and the lower portion is characterized by a second substrate diameter that is less than the first substrate diameter.

First claim

What is claimed:

1 . A sample carrier, comprising:
a cap formed from a substrate with a body disposed about an axis orthogonal to a top surface and a bottom surface of the substrate, said cap including:
an intermediate portion of the substrate body, wherein the intermediate portion comprises a first seating area with a first diameter configured to provide an interference fit within a first-sized sample chamber opening;
a lower portion of the substrate body, wherein the lower portion comprises a second seating area with a second diameter configured to provide an interference fit within a second-sized sample chamber opening; and
a sample transfer film applied to the bottom surface of the cap.

2 . The sample carrier of claim 1 , wherein the first diameter of the first seating area is greater than or equal to 0.250 inches.

3 . The sample carrier of claim 1 , wherein the second diameter of the second seating area is less than or equal to 0.200 inches.

4 . The sample carrier of claim 3 , wherein the second seating area comprises an arcuate surface.

5 . The sample carrier of claim 4 , wherein the arcuate surface has a radius of curvature of 0.018″.

6 . The sample carrier of claim 1 , wherein sample carrier further comprises:
an adjacent area disposed along the axis above the first seating area and defining an upper sidewall, the upper sidewall characterized by a first side wall diameter that is less than the first diameter of the first seating area.

7 . The sample carrier of claim 6 , wherein the upper side wall has an angle of at least 2 degrees relative to the axis.

8 . The sample carrier of claim 1 , wherein the sample carrier further comprises:
an adjacent area disposed along the axis above the second seating area and defining a lower sidewall, the lower sidewall characterized by a second side wall diameter that is less than the second diameter of the second seating area.

9 . The sample carrier of claim 1 , wherein the cap is configured to engage with a plurality of sample chambers of different volumes.

10 . The sample carrier of claim 9 , wherein a volume of the plurality of sample chambers is between 0.01 milliliters to 2.0 milliliters.

11 . The sample carrier of claim 10 , wherein the sample chamber is a 0.20 milliliter tube.

12 . The sample carrier of claim 10 , wherein the sample chamber is a 0.50 milliliter tube.

13 . The sample carrier of claim 1 , wherein the transfer film is configured to accommodate a biological sample.

14 . The sample carrier of claim 1 , wherein the transfer film is an ethylene vinyl acetate film.

15 . The sample carrier of claim 14 , wherein the ethylene vinyl acetate film contains a dye capable of absorbing energy from a laser source.

Assignees

• Life Technologies Corp

Inventors

• Colburn Joel
• Rath Mousumi
• Ghanbari Sarah

Classifications

• C12Q1/6806 Primary

  Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay (C12Q1/6804 takes precedence) · CPC title

• G01N2001/2886

  Laser cutting, e.g. tissue catapult · CPC title

• G01N2001/028

  Sampling from a surface, swabbing, vaporising · CPC title

• G01N1/34

  Purifying; Cleaning {(processes or apparatus for extracting or separating nucleic acids from biological samples C12N15/1003)} · CPC title

• B01L3/50825

  Closing or opening means, corks, bungs · CPC title