On-Chip Nanoscale Storage System Using Chimeric DNA

Patent metadata

Field	Value
Publication number	US-2021103824-A1
Application number	US-201916593450-A
Country	US
Kind code	A1
Filing date	Oct 4, 2019
Priority date	Oct 4, 2019
Publication date	Apr 8, 2021
Grant date	—

Abstract

The present disclosure provides systems and methods that can provide portable, real-time accessible DNA memories. An example DNA-based data storage system includes a loading region configured to receive a plurality of DNA-based data storage elements in a suspension fluid and a plurality of microtubes disposed in a capture/release region. The microtubes are configured to capture and release the DNA-based data storage elements. The DNA-based data storage system also includes a linearization region configured to linearize the DNA-based data storage elements and a readout region with a readout device configured to provide information indicative of the respective DNA-based data storage elements.

First claim

1 . A deoxyribonucleic acid (DNA)-based data storage element comprising:
a DNA backbone; and a plurality of non-natural nucleic acids bioconjugated to the DNA backbone.

2 . The DNA-based data storage element of claim 1 , wherein at least one of the non-natural nucleic acids comprise a peptide nucleic acid (PNA).

3 . The DNA-based data storage element of claim 2 , wherein the PNA comprises a peptide backbone and a plurality of natural nucleobase monomers.

4 . The DNA-based data storage element of claim 1 , wherein the DNA backbone comprises single-stranded DNA.

5 . The DNA-based data storage element of claim 1 , wherein the DNA backbone comprises double-stranded DNA.

6 . The DNA-based data storage element of claim 1 , wherein the plurality of non-natural nucleic acids comprises a structurally-defined branched polymer architecture.

7 . A microfluidic deoxyribonucleic acid (DNA)-based data storage system, comprising:
a loading region configured to receive a plurality of DNA-based data storage elements in a suspension fluid; a plurality of microtubes disposed in a capture/release region, wherein the microtubes are configured to capture and release the DNA-based data storage elements; a linearization region configured to linearize the DNA-based data storage elements; and a readout region with a readout device configured to provide information indicative of the respective DNA-based data storage elements.

8 . The DNA-based data storage system of claim 7 , wherein at least one microtube of the plurality of microtubes comprises a self-rolled microtube.

9 . The DNA-based data storage system of claim 8 , wherein, in an initial condition, the self-rolled microtube comprises:
a substrate; a sacrificial etch material overlaying the substrate; a compressive layer overlaying the sacrificial etch material; a tensile layer overlaying the compressive layer; and a plurality of electrodes.

10 . The DNA-based data storage system of claim 9 , wherein, in a rolled condition, the self-rolled microtube comprises:
at least a portion of the tensile and compressive layers rolled into a tubular shape having a diameter of less than 10 microns.

11 . The DNA-based data storage system of claim 7 , wherein the linearization region comprises an array of linearization structures arranged between the capture/release region and the readout region.

12 . The DNA-based data storage system of claim 7 , wherein the readout device comprises a solid-state nanopore device.

13 . The DNA-based data storage system of claim 7 , wherein the readout device comprises a tandem mass spectrometry system.

14 . A method to synthesize a deoxyribonucleic acid (DNA)-based data storage element comprising:
selecting an abasic site of a DNA backbone; modifying the abasic site to be compatible with bioconjugation by way of cycloaddition; and performing a bioconjugation so as to add at least one non-natural functional group to the abasic site as modified.

15 . The method of claim 14 , wherein the bioconjugation comprises an azide-alkyne Huisgen-type cycloaddition.

16 . The method of claim 14 , wherein modifying the abasic site is performed so as to form a bioconjugation click chemistry target.

17 . The method of claim 14 , wherein performing the bioconjugation comprises adding at least one peptide nucleic acid (PNA), wherein the PNA comprises a peptide backbone and a plurality of natural nucleobase monomers.

18 . A method comprising:
dispensing a plurality of deoxyribonucleic acid (DNA)-based data storage elements in a suspension fluid into a loading region of a microfluidic DNA-based data storage system; causing at least one microtube of a plurality of microtubes disposed in a capture/release region of the DNA-based data storage system to capture at least one DNA-based data storage element; causing the at least one microtube to release the at least one DNA-based data storage element; and receiving, from a readout device disposed proximate to a readout region of the DNA-based data storage system, information indicative of data stored with the at least one DNA-based data storage element.

19 . The method of claim 18 , wherein causing the at least one microtube to capture or release the at least one DNA-based data storage element comprises biasing a plurality of electrodes of the at least one microtube so as to capture or release the at least one DNA-based data storage element, respectively.

20 . The method of claim 18 , further comprising causing the at least one microtube to hold the at least one DNA-based data storage element within the at least one microtube.

Assignees

• Univ Illinois

Inventors

• Milenkovic Olgica
• Athreya Nagendra
• Khandelwal Apratim
• Leburton Jean-Pierre
• Li Xiuling
• Schroeder Charles
• Tabatabaei Kasra
• Li Bo

Classifications

• G06N20/00

  Machine learning · CPC title

• C40B40/06

  Libraries containing nucleotides or polynucleotides, or derivatives thereof · CPC title

• C12N2795/14131

  Uses of virus other than therapeutic or vaccine, e.g. disinfectant · CPC title

• C12N7/00 Primary

  Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof (preparing medicinal viral antigen or antibody compositions, e.g. virus vaccines, A61K39/00) · CPC title

• C12N2795/14142

  virus or viral particle as vehicle, e.g. encapsulating small organic molecule · CPC title