Linear displacement isothermal amplification method and application thereof
US-2024368679-A1 · Nov 7, 2024 · US
US2021055187A1 · US · A1
| Field | Value |
|---|---|
| Publication number | US-2021055187-A1 |
| Application number | US-201816958421-A |
| Country | US |
| Kind code | A1 |
| Filing date | Dec 27, 2018 |
| Priority date | Dec 29, 2017 |
| Publication date | Feb 25, 2021 |
| Grant date | — |
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This invention relates generally to characterizing bioaerosols and, more particularly, to a system for recovering, quantifying, identifying, and assessing the metabolic activities of bioaerosols based on their major biopolymer profiles (lipids, carbohydrate and protein) and more specific their genetic materials (DNA/RNA), such as airborne viruses, bacteria, fungi and pollens.
Opening claim text (preview).
1 - 19 . (canceled) 20 . A method of sampling, recovering and stabilizing bioaerosol materials comprising; a) providing; i) a condensation growth tube; and ii) an aerosol stream comprising water vapor and bioaerosol materials; b) directing said aerosol stream into said tube under said conditions that said vapor absorbs, adsorbs and/or condenses on said bioaerosol particles so as to form microdroplets; and c) collecting individual and/or a conglomerate of microdroplets into individual, sterile DNA-free containers containing a genomic, transcriptomic or proteomic preservative, wherein said microdroplets are simultaneously exposed to said preservative as they are collected. 21 . The method of claim 20 , wherein simultaneous preservation and capture occurs in the condensation growth tube. 22 . The method of claim 20 , wherein the method further comprises step d) recovery of bioaerosol particles as they exist in the air. 23 . The method of claim 22 , wherein said bioaerosol particles comprise microbial nucleic acids. 24 . The method of claim 20 , wherein preservation of said bioaerosol particles is quantitative. 25 . The method of claim 20 , wherein said condensation growth tube is RNA-free and DNA-free prior to use.
by sorption · CPC title
aerosol sampling devices · CPC title
Nucleic acid amplification reactions · CPC title
Atmospheric sampling · CPC title
with cooling means · CPC title
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