Imaging data processing apparatus and imaging data processing program

Patent metadata

Field	Value
Publication number	US-2021035299-A1
Application number	US-201816964522-A
Country	US
Kind code	A1
Filing date	Mar 30, 2018
Priority date	Mar 30, 2018
Publication date	Feb 4, 2021
Grant date	—

Abstract

In a data processing unit, alignment is performed by appropriately deforming one image among MS imaging images acquired from different samples so that positions and sizes on the MS imaging image are matched (S 1 to S 5 ). When the aligned image is displayed on a screen of a display unit and a user sets a region of interest on the image serving as a reference (S 6 ), a micro region including a center point within a range of the set region of interest is extracted in each of an image serving as the reference and an image not serving as the reference (S 7 ). In the image subjected to image deformation, although the shape of each micro region is distorted and micro regions are not arranged in an orderly grid manner, by assuming that the micro regions in which the center point is included within the range of the region of interest is included in the range of the region of interest, it is possible to perform a comparative analysis based on the data value within an appropriate micro region corresponding to the region of interest regardless of the image deformation.

First claim

1 . An imaging data processing apparatus for processing imaging data which is a set of data acquired by performing a predetermined analysis or observation in each of a plurality of micro regions within a two-dimensional measurement region on a sample, wherein the imaging data processing apparatus performs processing of imaging data acquired for each of a plurality of samples in which observation target sites are the same or similar to each other, or imaging data acquired by different analyses or observation methods or under different parameters for one sample in which observation target sites are the same, the imaging data processing apparatus comprising:
a) an image alignment processing unit configured to perform deformation processing accompanied by a movement of a position of each micro region in an image other than an image serving as a reference for one or a plurality of acquired samples so that the same or similar sites coincide with each other with one of a plurality of images generated based on a plurality of imaging data which is a processing target served as the reference; b) a region of interest setting reception unit configured to make a user set a region of interest on one image among an image served as the reference by the image alignment processing unit and an image deformed by the image alignment processing unit; and c) a region of interest correspondence micro region determination unit configured to determine a micro region included in the region of interest in each image by regarding a micro region existing within a frame of the region of interest in which a center point of the micro region moved at a time of the processing by the image alignment processing unit is set by the region of interest setting reception unit as a micro region included in the region of interest, in an image other than an image in which the region of interest is set by the region of interest setting acceptance unit among the plurality of images processed by the image alignment processing unit and the image served as the reference.

2 . An imaging data processing apparatus for processing imaging data which is a set of data acquired by performing a predetermined analysis or observation in each of a plurality of micro regions within a two-dimensional measurement region on a sample, wherein the imaging data processing apparatus performs processing of imaging data acquired for each of a plurality of samples in which observation target sites are the same or similar to each other, or imaging data acquired by different analyses or observation methods or under different parameters for one sample in which observation target sites are the same, the imaging data processing apparatus comprising:
a) an image alignment processing unit configured to perform deformation processing accompanied by a movement of a position of each micro region in an image other than an image serving as a reference for one or a plurality of acquired samples so that the same or similar sites coincide with each other with one of a plurality of images generated based on a plurality of imaging data which is a processing target served as the reference; b) a region of interest setting reception unit configured to make a user set a region of interest on one image among an image served as the reference by the image alignment processing unit and an image deformed by the image alignment processing unit; and c) a data value within region of interest operation unit configured to calculate a data value at a position corresponding to a center point of a micro region existing within a frame of the region of interest set by the region of interest setting reception unit on an image in which the region of interest is set, by interpolation processing using a data value at a position of the center point of each of a plurality of micro regions moved at the time of the processing by the image alignment processing unit, within a frame of the region of interest corresponding to the region of interest set by the region of interest setting reception unit on an image other than an image in which the region of interest is set by the region of interest setting acceptance unit among the plurality of images after the processing by the image alignment processing unit and the image served as the reference, and adopt the calculated value as a data value of each virtual micro region included in the region of interest on an image other than an image in which the region of interest is set.

3 . An imaging data processing apparatus for processing imaging data which is a set of data acquired by performing a predetermined analysis or observation in each of a plurality of micro regions within a two-dimensional measurement region on a sample, wherein the imaging data processing apparatus performs processing of imaging data acquired for each of a plurality of samples in which observation target sites are the same or similar to each other, or imaging data acquired by different analyses or observation methods or under different parameters for one sample in which observation target sites are the same, the imaging data processing apparatus comprising:
a) an image alignment processing unit configured to perform deformation processing accompanied by a movement of a position of each micro region in an image other than an image serving as a reference for one or a plurality of acquired samples so that the same or similar sites coincide with each other with one of a plurality of images generated based on a plurality of imaging data which is a processing target served as the reference; b) a region of interest setting reception unit configured to divide one image selected by a user among images deformed by the image alignment processing unit into predetermined micro regions, calculate a data value corresponding to a center point of each micro region by interpolation processing using a data value at a position of the center point of each of a plurality of micro regions moved at the time of the deformation processing by the image alignment processing unit, and display the image based on the data value calculated by the interpolation processing to allow the user to set a region of interest on the image; c) a data value within region of interest operation unit configured to calculate a data value at a position corresponding to a center point of a micro region existing within a frame of the region of interest set by the region of interest setting reception unit on an image in which the region of interest is set, by interpolation processing using a data value at a position of the center point of each of a plurality of micro regions moved at the time of the processing by the image alignment processing unit, within a frame of the region of interest corresponding to the region of interest set by the region of interest setting reception unit, for each image for an image at least subjected to the deformation processing by the image alignment processing unit, on an image other than an image in which the region of interest is set by the region of interest setting acceptance unit among the plurality of images after the processing by the image alignment processing unit and the image served as the reference, and adopt the calculated value as a data value of each virtual micro region included in the region of interest on an image.

4 . A computer-readable non-temporary recording medium storing an imaging data processing program for processing imaging data which is a set of data acquired by performing a predetermined analysis or observation in each of a plurality of micro regions within a two-dimensional measurement region on a sample, wherein the imaging data processing program performs processing of imaging data acquired for each of a plurality of samples in which observation target sites are the same or similar to each other, or imaging data acquired by different analyses or obser

Assignees

• Shimadzu Corp

Inventors

• Ikegami Masahiro

Classifications

• G06T2207/30024

  Cell structures in vitro; Tissue sections in vitro · CPC title

• G06T2207/20104

  Interactive definition of region of interest [ROI] · CPC title

• G06T7/337 Primary

  involving reference images or patches · CPC title

• G06T7/0014 Primary

  using an image reference approach · CPC title

• G06T2207/10056

  Microscopic image · CPC title