Synthetic nucleic acids having non-natural structures

Patent metadata

Field	Value
Publication number	US-2021017517-A1
Application number	US-202016931200-A
Country	US
Kind code	A1
Filing date	Jul 16, 2020
Priority date	Jul 16, 2019
Publication date	Jan 21, 2021
Grant date	—

Abstract

Nucleic acid compositions having nucleotide sequences that do not occur in nature are provided. Also provided are populations of different nucleic acids having universal adapters or universal primer binding sites. Methods of capturing, copying or amplifying nucleic acids of interest using universal adapters, universal primer binding sites and/or universal primers are also provided.

First claim

What is claimed is:

1 . A composition comprising an isolated nucleic acid molecule comprising the sequence or the complement of SEQ ID NO:1.

2 . The composition of claim 1 , wherein the 3′ end of the sequence of SEQ ID NO:1 is the 3′ end of a strand of the isolated nucleic acid molecule, or wherein the 3′ end of the complement of SEQ ID NO:1 is the 3′ end of a strand of the isolated nucleic acid molecule.

3 . The composition of claim 1 , wherein the isolated nucleic acid molecule is single stranded.

4 . The composition of claim 1 , wherein the isolated nucleic acid molecule is double stranded, thereby having a first strand and a second strand.

5 . The composition of claim 4 , wherein the first strand of the isolated nucleic acid molecule comprises the sequence of SEQ ID NO:1 and wherein the second strand of the isolated nucleic acid comprises the complement of SEQ ID NO:1.

6 . The composition of claim 1 , wherein the nucleic acid comprises DNA.

7 . The composition of claim 1 , wherein the nucleic acid comprises RNA.

8 . The composition of claim 1 , wherein the nucleic acid comprises a linker moiety, the linker moiety being exogenous to the nucleic acid.

9 . The composition of claim 8 , wherein the linker moiety attaches the nucleic acid to a label moiety, the label moiety being exogenous to the nucleic acid.

10 . The composition of claim 8 , wherein the exogenous linker moiety attaches the nucleic acid to a solid phase support.

11 . The composition of claim 10 , wherein the solid phase support comprises a bead.

12 . The composition of claim 10 , wherein the solid phase support comprises a site of a nucleic acid array.

13 . The composition of claim 1 , wherein the nucleic acid molecule is in solution phase.

14 . The composition of claim 1 , wherein the isolated nucleic acid molecule further comprises the sequence or the complement of SEQ ID NO:3.

15 . The composition of claim 14 , wherein the 3′ end of the sequence of SEQ ID NO:3 is the 3′ end of a strand of the isolated nucleic acid molecule, or wherein the 3′ end of the complement of SEQ ID NO:3 is the 3′ end of a strand of the isolated nucleic acid molecule.

16 . The composition of claim 14 , wherein the isolated nucleic acid molecule is double stranded, thereby having a first strand and a second strand.

17 . The composition of claim 16 , wherein the first strand of the isolated nucleic acid molecule comprises the sequence of SEQ ID NO:3 and wherein the second strand of the isolated nucleic acid comprises the complement of SEQ ID NO:3.

18 . The composition of claim 17 , wherein the first strand of each of the nucleic acid molecules comprises the sequence of SEQ ID NO:1 and wherein the second strand of each of the nucleic acid molecules comprises the complement of SEQ ID NO:1.

19 . The composition of claim 17 , wherein the first strand of each of the nucleic acid molecules comprises the complement of SEQ ID NO:1 and wherein the second strand of each of the nucleic acid molecules comprises the sequence of SEQ ID NO:1.

20 . The composition of claim 1 , wherein the isolated nucleic acid molecule further comprises a variant of SEQ ID NO:3 having 1, 2, 3 or 4 nucleotide substitutions.

21 . The composition of claim 20 , wherein the variant is SEQ ID NO:13, SEQ ID NO:14 or SEQ ID NO:15.

22 . A population of different nucleic acid molecules, comprising a plurality of nucleic acid molecules,
wherein each of the nucleic acid molecules comprises a universal sequence region and a variable sequence region, wherein the sequence of the variable sequence region differs between different nucleic acid molecules in the population, wherein the sequence of the universal sequence region is the same for the nucleic acid molecules in the population, and wherein the universal sequence region comprises the sequence or the complement of SEQ ID NO:1.

23 . A method for preparing a nucleic acid library, comprising
attaching universal adapters to nucleic acid fragments from a genome, thereby producing a library of nucleic acid molecules, wherein each of the nucleic acid molecules in the library comprises:
(i) a variable sequence region that differs between the nucleic acid molecules in the library, and
(ii) a universal sequence region that is the same for the nucleic acid molecules in the library, wherein the universal sequence region comprises the sequence or the complement of SEQ ID NO:1.

Assignees

• Omniome Inc

Inventors

• Oliphant Arnold
• Yu James

Classifications

• C12N15/1093

  General methods of preparing gene libraries, not provided for in other subgroups · CPC title

• C40B40/06 Primary

  Libraries containing nucleotides or polynucleotides, or derivatives thereof · CPC title

• C12N15/111

  General methods applicable to biologically active non-coding nucleic acids · CPC title

• C12N15/1096 Primary

  cDNA Synthesis; Subtracted cDNA library construction, e.g. RT, RT-PCR · CPC title

• C40B40/08

  Libraries containing RNA or DNA which encodes proteins, e.g. gene libraries · CPC title