Methods and compositions for modifying assembly-activating protein (AAP)-dependence of viruses
US-11034732-B2 · Jun 15, 2021 · US
Ancestral Virus Sequences and Uses Thereof
US2021017235A1 · US · A1
| Field | Value |
|---|---|
| Publication number | US-2021017235-A1 |
| Application number | US-202016989454-A |
| Country | US |
| Kind code | A1 |
| Filing date | Aug 10, 2020 |
| Priority date | Jul 30, 2015 |
| Publication date | Jan 21, 2021 |
| Grant date | — |
How to read this patent
A practical reading order for non-experts. Skip the full description unless you need deep technical detail.
- Title
What the patent document calls the invention.
- Abstract
A short plain-language summary of the technical disclosure.
- Assignees and inventors
Who owns or filed the patent and who is credited as inventor.
- Key dates
Filing, priority, publication, and grant dates set the timeline.
- First independent claim
The legal scope of protection — read this for what is actually claimed.
- CPC / IPC classifications
Technology tags used to group this patent with similar filings.
- Citations and related patents
Prior art links and similar publications in this corpus.
Abstract
Official abstract text for this publication.
Methods are described for predicting ancestral sequences for viruses or portions thereof. Also described are predicted ancestral sequences for adeno-associated virus (AAV) capsid polypeptides. The disclosure also provides methods of gene transfer and methods of vaccinating subjects by administering a target antigen operably linked to the AAV capsid polypeptides.
First claim
Opening claim text (preview).
What is claimed is: 1 . An adeno-associated virus (AAV) capsid polypeptide having the amino acid sequence shown in SEQ ID NO: 42. 2 . The AAV capsid polypeptide of claim 1 , wherein the AAV capsid polypeptide or a virus particle comprising the AAV capsid polypeptide exhibits about the same or a lower seroprevalence than does an AAV9 capsid polypeptide or a virus particle comprising an AAV9 capsid polypeptide. 3 . The AAV capsid polypeptide of claim 1 , wherein the AAV capsid polypeptide or a virus particle comprising the AAV capsid polypeptide is neutralized to a similar or lesser extent by human serum than is an AAV9 capsid polypeptide or a virus particle comprising an AAV9 capsid polypeptide. 4 . The AAV capsid polypeptide of claim 1 , wherein the AAV capsid polypeptide is purified. 5 . The AAV capsid polypeptide of claim 1 encoded by the nucleic acid sequence shown in SEQ ID NO: 43. 6 . A nucleic acid molecule encoding an adeno-associated virus (AAV) capsid polypeptide having the nucleic acid sequence shown in SEQ ID NO: 43. 7 . A vector comprising the nucleic acid molecule of claim 6 . 8 . A host cell comprising the vector of claim 7 . 9 . A purified virus particle comprising the AAV capsid polypeptide of claim 1 . 10 . The purified virus particle of claim 9 , further comprising a transgene. 11 . A method of gene transfer and/or vaccination with a transgene, the method comprising administering the virus particle of claim 10 to a subject in need of gene transfer or vaccination, wherein the virus particle exhibits about the same or a lower seroprevalence than does an AAV9 virus particle. 12 . The method of claim 11 , wherein the virus particle is neutralized to the same or to a lesser extent by human serum than is an AAV9 virus particle. 13 . A method of vaccinating a subject, the method comprising administering a target antigen operably linked to the AAV capsid polypeptide of claim 1 to a subject in need of vaccination, wherein the AAV capsid polypeptide exhibits about the same or a lower seroprevalence than does an AAV9 capsid polypeptide. 14 . The method of claim 13 , wherein the AAV capsid polypeptide is neutralized to the same or to a lesser extent by human serum than is an AAV9 capsid polypeptide.
Assignees
Inventors
Classifications
New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes · CPC title
Methods of production or purification of viral material · CPC title
viral genome or elements thereof as genetic vector · CPC title
Specific hybridization probes · CPC title
- C07K14/005Primary
from viruses · CPC title
Patent family
Related publications grouped by family.
External sources
Frequently asked questions
Answers are generated from the same data shown on this page.
- What does patent US2021017235A1 cover?
- Methods are described for predicting ancestral sequences for viruses or portions thereof. Also described are predicted ancestral sequences for adeno-associated virus (AAV) capsid polypeptides. The disclosure also provides methods of gene transfer and methods of vaccinating subjects by administering a target antigen operably linked to the AAV capsid polypeptides.
- Who is the assignee on this patent?
- Massachusetts Eye & Ear Infirmary, Schepens Eye Res Inst
- What technology area does this patent fall under?
- Primary CPC classification C07K14/005. Mapped technology areas include Chemistry & Metallurgy.
- When was this patent published?
- Publication date Thu Jan 21 2021 00:00:00 GMT+0000 (Coordinated Universal Time) (A1). Legal status and post-grant events are not shown on this page.
- What related patents are in patentsdb?
- We list 2 related publications on this page (citations in our corpus or others sharing the same primary CPC).