Plants having increased tolerance to herbicides

Patent metadata

Field	Value
Publication number	US-2021002662-A1
Application number	US-201816767917-A
Country	US
Kind code	A1
Filing date	Nov 28, 2018
Priority date	Nov 29, 2017
Publication date	Jan 7, 2021
Grant date	—

Abstract

The present invention refers to a method for controlling undesired vegetation at a plant cultivation site, the method comprising the steps of providing, at said site, a plant that comprises at least one nucleic acid comprising a nucleotide sequence encoding protoporphyrinogen oxidase (PPO) which is resistant or tolerant to a PPO-inhibiting herbicide by applying to said site an effective amount of said herbicide. The invention further refers to plants comprising wild-type or mutated PPO enzymes, and methods of obtaining such plants.

First claim

1 . A method for controlling undesired vegetation at a plant cultivation site, the method comprising the steps of:
a) providing, at said site, a plant that comprises at least one nucleic acid comprising a nucleotide sequence encoding a protoporphyrinogen oxidase (PPO) polypeptide which is resistant or tolerant to a “PPO inhibiting herbicide”; b) applying to said site an effective amount of said herbicide,
wherein the PPO inhibiting herbicide is a uracilpyridine of formula (I)

wherein the substituents have the following meanings:
R 1 hydrogen, NH 2 , C 1 -C 6 -alkyl or C 3 -C 6 -alkynyl;
R 2 hydrogen, C 1 -C 6 -alkyl or C 1 -C 6 -haloalkyl;
R 3 hydrogen or C 1 -C 6 -alkyl;
R 4 H or halogen;
R 5 halogen, CN, NO 2 , NH 2 , CF 3 or C(═S)NH 2 ;
R 6 H, halogen, CN, C 1 -C 3 -alkyl, C 1 -C 3 -haloalkyl, C 1 -C 3 -alkoxy, C 1 -C 3 -haloalkoxy, C 1 -C 3 -alkylthio, (C 1 -C 3 -alkyl)amino, di(C 1 -C 3 -alkyl)amino, C 1 -C 3 -alkoxy- C 1 -C 3 -alkyl, C 1 -C 3 -alkoxycarbonyl;
R 7 H, halogen, C 1 -C 3 -alkyl, C 1 -C 3 -alkoxy;
R 8 OR 9 , SR 9 , NR 10 R 11 , NR 9 OR 9 , NR 9 S(O) 2 R 10 or NR 9 S(O) 2 NR 10 R 11 , wherein
R 9 is hydrogen, C 1 -C 6 -alkyl, C 3 -C 6 -alkenyl, C 3 -C 6 -alkynyl, C 1 -C 6 -haloalkyl, C 3 -C 6 -haloalkenyl, C 3 -C 6 -haloalkynyl, C 1 -C 6 -cyanoalkyl, C 1 -C 6 -alkoxy- C 1 -C 6 -alkyl, C 1 -C 6 -alkoxy-C 1 -C 6 -alkoxy- C 1 -C 6 -alkyl, di(C 1 -C 6 -alkoxy) C 1 -C 6 -alkyl, C 1 -C 6 -haloalkoxy- C 1 -C 6 -alkyl, C 3 -C 6 -alkenyloxy- C 1 -C 6 -alkyl, C 3 -C 6 -haloalkenyloxy- C 1 -C 6 -alkyl, C 3 -C 6 -alkenyloxy- C 1 -C 6 -alkoxy- C 1 -C 6 -alkyl, C 1 -C 6 -alkylthio- C 1 -C 6 -alkyl, C 1 -C 6 -alkylsulfinyl- C 1 -C 6 -alkyl, C 1 -C 6 -alkylsulfonyl- C 1 -C 6 -alkyl, C 1 -C 6 -alkylcarbonyl- C 1 -C 6 -alkyl, C 1 -C 6 -alkoxycarbonyl- C 1 -C 6 -alkyl, C 1 -C 6 -haloalkoxycarbonyl- C 1 -C 6 -alkyl, C 3 -C 6 -alkenyloxycarbonyl- C 1 -C 6 -alkyl, C 3 -C 6 -alkynyloxycarbonyl- C 1 -C 6 -alkyl, amino, (C 1 -C 6 -alkyl)amino, di(C 1 -C 6 -alkyl)amino, (C 1 -C 6 -alkylcarbonyl)amino, amino- C 1 -C 6 -alkyl, (C 1 -C 6 -alkyl)amino- C 1 -C 6 -alkyl, di(C 1 -C 6 -alkyl)amino- C 1 -C 6 -alkyl, aminocarbonyl- C 1 -C 6 -alkyl, (C 1 -C 6 -alkyl)aminocarbonyl- C 1 -C 6 -alkyl, di(C 1 -C 6 -alkyl)aminocarbonyl- C 1 -C 6 -alkyl,
—N═CR 12 R 13 , wherein R 12 and R 13 independently of one another are H, C 1 -C 4 -alkyl or phenyl;
C 3 -C 6 -cycloalkyl, C 3 -C 6 -cycloalkyl- C 1 -C 6 -alkyl, C 3 -C 6 -heterocyclyl, C 3 -C 6 -heterocyclyl- C 1 -C 6 -alkyl, phenyl, phenyl- C 1 -C 4 -alkyl or a 5- or 6 membered heteroaryl,
wherein each cycloalkyl, heterocyclyl, phenyl or heteroaryl ring can be substituted by one to four substituents selected from R 14 or a 3- to 7-membered carbocyclus,
which carbocyclus optionally has in addition to carbon atoms one or two ring members selected from the group consisting of
—N(R 12 )—, —N═N—, —C(═O)—, —O— and —S—, and
which carbocyclus is optionally substituted with one to four substituents selected from R 14 ;
wherein R 14 is halogen, NO 2 , CN, C 1 -C 4 -alkyl, C 1 -C 4 -halo-,alkyl, C 1 -C 4 -alkoxy or C 1 -C 4 -alkoxy-,carbonyl;
R 10 , R 11 independently of one another are R 9 , or together form a 3- to 7-membered carbocyclus,
which carbocyclus optionally has in addition to carbon atoms one or two ring members selected from the group consisting of —N(R 12 )—, —N═N—, —C(═O)—, —O— and —S—, and which carbocyclus is optionally substituted with one to four substituents selected from R 14 ;
n 1 to 3;
Q CH 2 , O, S, SO, SO 2 , NH or (C 1 -C 3 -alkyl)N;
W O or S;
X NH, NCH 3 , 0 or S;
Y O or S;
Z phenyl, pyridyl, pyridazinyl, pyrimidinyl or pyrazinyl,
each of which is optionally substituted by 1 to 4 substituents selected from the group consisting of halogen, CN, C 1 -C 6 -alkyl, C 1 -C 6 -haloalkyl, C 1 -C 6 -alkoxy, C 1 -C 6 -haloalkoxy;
including their agriculturally acceptable salts or derivatives, provided the compounds of formula (I) have a carboxyl group,

and wherein the effective amount of said herbicide does not kill or inhibit the growth of the herbicide-tolerant plant of a).

2 . The method according to claim 1 , wherein the herbicide resistant or tolerant PPO polypeptide comprises one or more of the following motifs 1, 2, and/or 3:
a. Motif 1: SQ[N/K/H]KRYI, wherein the Arg at position 5 within said motif is substituted by any other amino acid; b. Motif 2: TLGTLFSS, wherein the Leu at position 2, and/or the Gly at position 3, and/or the Leu at position 5 within said motif is substituted by any other amino acid; c. Motif 3: [F/Y]TTF[V/I]GG, wherein the Phe at position 4 within said motif is substituted by any other amino acid.

3 . The method according to claim 1 , wherein the herbicide resistant or tolerant PPO polypeptide comprises a variant of the amino acid sequence of SEQ ID NO: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63, 64, 65, 66, 67, 68, 69, 70, 71, 72, 73, 74, 75, 76, 77, 78, 79, 80, 81, 82, 83, 84, 85, 86, 87, 88, 89, 90, 91, 92, 93, 94, 95, 96, 97, 98, 99, 100, 101, 102, 103, 104, 105, 106, 107, 108, 109, 110, 111, 112, 113, 114, 115, 116, 117, 577, 579, 581, 583, 585, 587, 589, 591, 593, 595, 597, 599, 601, 603, 605, 607, 609, 611, 613, 615, 617, 619, 621, 623, 625, 627, or 637, which variant comprises one or more of the following substitutions:
a. the amino acid corresponding to Arg128 of SEQ ID NO: 1 is substituted by any other amino acid. b. The amino acid corresponding to Gly211 of SEQ ID NO: 1 is substituted by any other amino acid c. the amino acid corresponding to Leu397 of SEQ ID NO: 1 is substituted by any other amino acid. d. the amino acid corresponding to Gly398 of SEQ ID NO: 1 is substituted by any other amino acid e. the amino acid corresponding to Leu400 of SEQ ID NO: 1 is substituted by any other amino acid f. the amino acid corresponding to Phe420 of SEQ ID NO: 1 is substituted by any other amino acid.

4 . The method according to claim 1 , wherein the herbicide resistant or tolerant PPO polypeptide comprises the amino acid sequence of SEQ ID NO: 265, 266, 267, 268, 269, 270, 271, 272, 273, 274, 275, 276, 277, 278, 279, 280, 281, 282, 283, 284, 285, 286, 287, 288, 289, 290, 291, 292, 293, 294, 295, 296, 297, 298, 299, 300, 301, 302, 303, 304, 305, 306, 307, 308, 309, 310, 311, 312, 313, 314, 315, 316, 317, 318, 319, 320, 321, 322, 323, 324, 325, 326, 327328, 329, 330, 331, 332, 333, 334, 335, 336, 337, 338, 339, 340, 341, 342, 343, 344, 345, 346, 347, 348, 349, 350, 351, 352, 353, 354, 355, 356, 357, 358, 359, 360, 361, 362, 363, 364, 365, 366, 367, 368, 369, 370, 371, 372, 373, 374, 375, 376, 377, 378, 379, 380, 381, 382, 383, 384, 385, 386, 387, 388, 389, 390, 391, 392, 393, 394, 395, 396, 397, 398, 399, 400, 401, 402, 403, 404, 405, 406, 407, 408, 409, 410, 411, 412, 413, 414, 415, 416, 417, 418, 419, 420, 421, 422, 423, 424, 425, 426, 427, 428, 429, 430, 431, 432, 433, 434, 435, 436, 437, 438, 439, 440, 441, 442, 443, 444, 445, 446, 447, 448, 449, 450, 451, 452, 453, 454, 455, 456, 457, 458, 459, 460, 461, 462, 463, 464, 465, 466, 467, 468, 469, 470, 471, 472, 473, 474, 475, 476, 477, 478, 479, 480, 481, 482, 483, 484, 485, 486, 487, 488, 489, 490, 491, 492, 493, 494, 495, 496, 497, 498, 499, 500, 501, 502, 503, 504, 505, 506, 507, 508, 509, 510, 511, 512, 513, 514, 515, 516, 517, 518, 519, 520, 521, 522, 523, 524, 525, 526, 527, 528, 529, 530, 531, 532, 533,

Assignees

• Basf Se

Inventors

• Aponte Raphael
• Seiser Tobias
• Tresch Stefan
• Bowerman Peter Alexander
• Parra Rapado Liliana
• Witschel Matthias
• Souillart Laetitia
• Johannes Manuel
• Mietzner Thomas
• Paulik Jill Marie

Classifications

• C12Y103/03004

  Protoporphyrinogen oxidase (1.3.3.4) · CPC title

• C12N15/8274 Primary

  for herbicide resistance · CPC title

• C12N9/001 Primary

  acting on the CH-CH group of donors (1.3) · CPC title

• A01N43/54

  1,3-Diazines; Hydrogenated 1,3-diazines · CPC title

• A01P13/00

  Herbicides; Algicides · CPC title