Method for determining ultraviolet light sensitivity

Patent metadata

Field	Value
Publication number	US-2020393380-A1
Application number	US-201816958354-A
Country	US
Kind code	A1
Filing date	Dec 28, 2018
Priority date	Dec 28, 2017
Publication date	Dec 17, 2020
Grant date	—

Abstract

The present invention provides a method for determining a sensitivity to ultraviolet light non-invasively and immediately. A method for determining a UV sensitivity is provided involving: a step of irradiating the skin of a test subject with ultraviolet light to determine the UV sensitivity using the amount of biophotons to be detected within a specific period after the irradiation, wherein 50% or more of the specific period overlaps a period from 1 to 3 minutes after the irradiation.

First claim

1 . A method for determining a UV sensitivity, comprising:
irradiating a skin of a test subject with ultraviolet light to determine the UV sensitivity using the amount of biophotons to be detected within a specific period after the irradiation, wherein 50% or more of the specific period overlaps a period from 1 to 3 minutes after the irradiation.

2 . The method according to claim 1 , wherein the specific period has a length of from 30 seconds to 3 minutes.

3 . The method according to claim 1 , wherein the specific period is 1 minute from 1 to 2 minutes after the UV irradiation, 1 minute from 2 to 3 minutes after the UV irradiation, or 2 minutes from 1 to 3 minutes after the UV irradiation.

4 . The method according to claim 1 , wherein the UV irradiation is irradiation with mixed ultraviolet light of A radiation and B radiation.

5 . The method according to claim 1 , wherein the UV irradiation is performed at a UV irradiation dose of from 300 to 8000 mJ/cm 2 .

6 . The method according to claim 1 , wherein the amount of biophotons is calculated based on the luminescence intensity of biophotons.

7 . The method according to claim 1 , wherein the UV sensitivity is erythema formation by ultraviolet light.

8 . A method for evaluating or searching for a UV sensitivity-reducing agent, comprising:
administering a test substance to a subject or bringing the test substance into contact with the subject, and irradiating the skin or skin cells of the subject with ultraviolet light to evaluate the test substance using the amount of biophotons to be detected within a specific period after the irradiation, wherein 50% or more of the specific period overlaps a period from 1 to 3 minutes after the irradiation.

9 . The method according to claim 8 , wherein the subject is a human, cultured epidermal cells, a 3D skin model, or a cultured skin tissue.

10 . The method according to claim 8 , wherein the administration of the test substance to the subject or the contact of the test substance with the subject is performed at a specific frequency of once or multiple times within a specific administration or contact period provided before the UV irradiation.

11 . The method according to claim 8 , wherein the administration of the test substance to the subject is performed during an administration period of one day or more and 6 months or less, at an administration frequency of once or more per day.

12 . The method according to claim 8 , wherein the contact of the test substance with the cultured epidermal cells, the 3D skin model, or the cultured skin tissue is performed for a contact period of 1 hour or more and 72 hours or less, at a contact frequency of once or more during the contact period.

13 . The method according to claim 8 , wherein the amounts of biophotons are compared between a higher-concentration test substance-administered group and a lower-concentration test substance-administered group; between a test substance-administered group and a placebo-administered group; between a test substance-administered group and a non-administered group; or between before and after administration of each test substance.

Assignees

• Kao Corp

Inventors

• Gabe Yu
• Kasamatsu Shinya
• Osanai Osamu
• Takeda Katsuya
• Nakajima Yoko

Classifications

• G01N33/15

  Medicinal preparations {; Physical properties thereof, e.g. dissolubility} · CPC title

• A61B5/445

  Evaluating skin irritation or skin trauma, e.g. rash, eczema, wound, bed sore · CPC title

• A61B5/0075

  by spectroscopy, i.e. measuring spectra, e.g. Raman spectroscopy, infrared absorption spectroscopy (A61B5/0071 takes precedence) · CPC title

• G01N2800/20

  Dermatological disorders · CPC title

• G01N33/6881

  from skin · CPC title