Filovirus Consensus Antigens, Nucleic Acid Constructs And Vaccines Made Therefrom, And Methods Of Using Same

Patent metadata

Field	Value
Publication number	US-2020390880-A1
Application number	US-202017004818-A
Country	US
Kind code	A1
Filing date	Aug 27, 2020
Priority date	Apr 12, 2012
Publication date	Dec 17, 2020
Grant date	—

Abstract

Nucleic acid molecules and compositions comprising one or more nucleic acid sequences that encode a consensus filovirus immunogen including a consensus Marburgvirus filovirus glycoprotein MARV GP immunogen, a consensus Ebolavirus Sudan filovirus glycoprotein SEBOV GP immunogen and a consensus Ebolavirus Zaire glycoprotein ZEBOV GP immunogen are disclosed. The coding sequences optionally include operable linked coding sequence that encode a signal peptide. Immunomodulatory methods and methods of inducing an immune response against filovirus, particularly Marburgvirus, Ebolavirus Sudan and Ebolavirus Zaire are disclosed. Method of preventing filovirus infection, particularly infection by Marburgvirus, Ebolavirus Sudan and Ebolavirus Zaire and methods of treating individuals infected with filovirus infection, particularly infection by Marburgvirus, Ebolavirus Sudan and Ebolavirus Zaire are disclosed. Consensus filovirus proteins are disclosed.

First claim

1 . A composition comprising:
a) a nucleic acid sequence that encodes a consensus Zaire ebolavirus envelope glycoprotein immunogen, the amino acid sequence of the consensus Zaire ebolavirus envelope glycoprotein immunogen selected from the group consisting of: SEQ ID NO:1 (ZEBOV CON), a fragment of SEQ ID NO:1 that comprises 600 or more amino acids, an amino acid sequence that is 95% homologous to SEQ ID NO:1, a fragment of an amino acid sequence that is 95% homologous to SEQ ID NO:1 that comprises 600 or more amino acids; SEQ ID NO:1 (ZEBOV CON) linked to an IgE signal peptide, a fragment of SEQ ID NO:1 that comprises 600 or more amino acids linked to an IgE signal peptide, an amino acid sequence that is 95% homologous to SEQ ID NO:1 linked to an IgE signal peptide, and a fragment of an amino acid sequence that is 95% homologous to SEQ ID NO:1 that comprises 600 or more amino acids linked to an IgE signal peptide; b) a nucleic acid sequence that encodes a consensus Sudan ebolavirus envelope glycoprotein immunogen, the amino acid sequence of the consensus Sudan ebolavirus envelope glycoprotein selected from the group consisting of: SEQ ID NO:2 (SUDV CON), a fragment of SEQ ID NO:2 that comprises 600 or more amino acids, an amino acid sequence that is 95% homologous to SEQ ID NO:2, a fragment of an amino acid sequence that is 95% homologous to SEQ ID NO:2 that comprises 600 or more amino acids, SEQ ID NO:2 (SUDV CON) linked to an IgE signal peptide, a fragment of SEQ ID NO:2 that comprises 600 or more amino acids linked to an IgE signal peptide, an amino acid sequence that is 95% homologous to SEQ ID NO:2 linked to an IgE signal peptide, and a fragment of an amino acid sequence that is 95% homologous to SEQ ID NO:2 that comprises 600 or more amino acids linked to an IgE signal peptide; and c) a nucleic acid sequence that encodes a Marburg marburgvirus Angola 2005 envelope glycoprotein immunogen, the amino acid sequence of the Marburg marburgvirus Angola 2005 envelope glycoprotein immunogen selected from the group consisting of: SEQ ID NO:3 (MARV), a fragment of SEQ ID NO:3 that comprises 600 or more amino acids, an amino acid sequence that is 95% homologous to SEQ ID NO:3, a fragment of an amino acid sequence that is 95% homologous to SEQ ID NO:3 that comprises 600 or more amino acids, SEQ ID NO:3 (MARV) linked to an IgE signal peptide, a fragment of SEQ ID NO:3 that comprises 600 or more amino acids linked to an IgE signal peptide, an amino acid sequence that is 95% homologous to SEQ ID NO:3 linked to an IgE signal peptide, and a fragment of an amino acid sequence that is 95% homologous to SEQ ID NO:3 that comprises 600 or more amino acids linked to an IgE signal peptide.

2 . The composition of claim 1 comprising: a first plasmid that comprises a nucleic acid sequence that encodes the consensus Zaire ebolavirus envelope glycoprotein immunogen; a second plasmid that comprises a nucleic acid sequence that encodes the consensus Sudan ebolavirus envelope glycoprotein immunogen; and a third plasmid that comprises a nucleic acid sequence that encodes the Marburg marburgvirus Angola 2005 envelope glycoprotein immunogen.

3 . The composition of claim 1 wherein:
a) the amino acid sequence of the consensus Zaire ebolavirus envelope glycoprotein immunogen is selected from the group consisting of: SEQ ID NO:1 (ZEBOV CON), a fragment of SEQ ID NO:1 that comprises 630 or more amino acids, an amino acid sequence that is 98% homologous to SEQ ID NO:1, a fragment of an amino acid sequence that is 98% homologous to SEQ ID NO:1 that comprises 630 or more amino acids; SEQ ID NO:1 (ZEBOV CON) linked to an IgE signal peptide, a fragment of SEQ ID NO:1 that comprises 630 or more amino acids linked to an IgE signal peptide, an amino acid sequence that is 98% homologous to SEQ ID NO:1 linked to an IgE signal peptide, and a fragment of an amino acid sequence that is 98% homologous to SEQ ID NO:1 that comprises 630 or more amino acids linked to an IgE signal peptide;
b) the amino acid sequence of the consensus Sudan ebolavirus envelope glycoprotein is selected from the group consisting of: SEQ ID NO:2 (SUDV CON), a fragment of SEQ ID NO:2 that comprises 630 or more amino acids, an amino acid sequence that is 98% homologous to SEQ ID NO:2, a fragment of an amino acid sequence that is 98% homologous to SEQ ID NO:2 that comprises 630 or more amino acids, SEQ ID NO:2 (SUDV CON) linked to an IgE signal peptide, a fragment of SEQ ID NO:2 that comprises 630 or more amino acids linked to an IgE signal peptide, an amino acid sequence that is 98% homologous to SEQ ID NO:2 linked to an IgE signal peptide, and a fragment of an amino acid sequence that is 98% homologous to SEQ ID NO:2 that comprises 630 or more amino acids linked to an IgE signal peptide; and
c) the amino acid sequence of the Marburg marburgvirus Angola 2005 envelope glycoprotein immunogen is selected from the group consisting of: SEQ ID NO:3 (MARV), a fragment of SEQ ID NO:3 that comprises 635 or more amino acids, an amino acid sequence that is 98% homologous to SEQ ID NO:3, a fragment of an amino acid sequence that is 98% homologous to SEQ ID NO:3 that comprises 635 or more amino acids, SEQ ID NO:3 (MARV) linked to an IgE signal peptide, a fragment of SEQ ID NO:3 that comprises 635 or more amino acids linked to an IgE signal peptide, an amino acid sequence that is 98% homologous to SEQ ID NO:3 linked to an IgE signal peptide, and a fragment of an amino acid sequence that is 98% homologous to SEQ ID NO:3 that comprises 635 or more amino acids linked to an IgE signal peptide.

4 . The composition of claim 3 comprising: a first plasmid that comprises a nucleic acid sequence that encodes the consensus Zaire ebolavirus envelope glycoprotein immunogen; a second plasmid that comprises a nucleic acid sequence that encodes the consensus Sudan ebolavirus envelope glycoprotein immunogen; and a third plasmid that comprises a nucleic acid sequence that encodes the Marburg marburgvirus Angola 2005 envelope glycoprotein immunogen.

5 . The composition of claim 3 wherein:
a) the amino acid sequence of the consensus Zaire ebolavirus envelope glycoprotein immunogen is selected from the group consisting of: SEQ ID NO:1 (ZEBOV CON), a fragment of SEQ ID NO:1 that comprises 660 or more amino acids, an amino acid sequence that is 99% homologous to SEQ ID NO:1, a fragment of an amino acid sequence that is 99% homologous to SEQ ID NO:1 that comprises 660 or more amino acids; SEQ ID NO:1 (ZEBOV CON) linked to an IgE signal peptide, a fragment of SEQ ID NO:1 that comprises 660 or more amino acids linked to an IgE signal peptide, an amino acid sequence that is 99% homologous to SEQ ID NO:1 linked to an IgE signal peptide, and a fragment of an amino acid sequence that is 99% homologous to SEQ ID NO:1 that comprises 660 or more amino acids linked to an IgE signal peptide;
b) the amino acid sequence of the consensus Sudan ebolavirus envelope glycoprotein is selected from the group consisting of: SEQ ID NO:2 (SUDV CON), a fragment of SEQ ID NO:2 that comprises 660 or more amino acids, an amino acid sequence that is 99% homologous to SEQ ID NO:2, a fragment of an amino acid sequence that is 99% homologous to SEQ ID NO:2 that comprises 660 or more amino acids, SEQ ID NO:2 (SUDV CON) linked to an IgE signal peptide, a fragment of SEQ ID NO:2 that comprises 660 or more amino acids linked to an IgE signal peptide, an amino acid sequence that is 99% homologous to SEQ ID NO:2 linked to an IgE signal peptide, and a fragment of an amino acid sequence that is 99% homologous to SEQ ID NO:2 that comprises 660 or more amino acids linked to an IgE signal peptide; and
c) the amino acid sequence of the Marburg marburgvirus Angola 2005 envelope glycoprotein immunogen is selected from the group consisting of: SEQ ID NO:3 (MARV), a fragment of SEQ ID NO:3 th

Assignees

• Univ Pennsylvania

Inventors

• Weiner David B
• Shedlock Devon

Classifications

• C12N2760/14222

  New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes · CPC title

• C12N2760/14122

  New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes · CPC title

• A61K2039/70

  Multivalent vaccine · CPC title

• A61K2039/545

  characterised by the dose, timing or administration schedule · CPC title

• C12N2760/14234

  Use of virus or viral component as vaccine, e.g. live-attenuated or inactivated virus, VLP, viral protein · CPC title