Grp78-derived peptide for identifying high-efficiency stem cells

Patent metadata

Field	Value
Publication number	US-2020369753-A1
Application number	US-201816755373-A
Country	US
Kind code	A1
Filing date	Oct 15, 2018
Priority date	Oct 13, 2017
Publication date	Nov 26, 2020
Grant date	—

Abstract

The present invention relates to a GRP78-derived peptide for screening highly efficient stem cells and the use thereof, and more particularly, to screening highly efficient stem cells using, as a marker, a GRP78-derived peptide capable of binding to the binding domain of GRP78 protein on the cell surface. According to the present invention, the GRP78-derived peptide comprising only a specific amino acid sequence capable of recognizing highly efficient stem cells, among the amino acid sequence of GRP78, makes it possible to screen only non-senescent young stem cells. In addition, when stem cells are treated with the GRP78-derived peptide or the GRP78-derived peptide is introduced into stem cells, the efficiency of the stem cells can be increased. Thus, the GRP78-derived peptide is useful for the production of stem cell therapy products having excellent efficacy.

First claim

1 . A peptide for screening highly efficient stem cells, the peptide being represented by an amino acid sequence selected from the group consisting of SEQ ID NOs: 1 to 18.

2 . The peptide of claim 1 , wherein the peptide is derived from GRP78.

3 . The peptide of claim 1 , wherein the peptides represented by the amino acid sequences of SEQ ID NOs: 1 to 3 recognize the 1-32 site of GRP78.

4 . The peptide of claim 1 , wherein the peptides represented by the amino acid sequences of SEQ ID NOs: 4 to 6 recognize the phosphorylation or oxidation site of the GRP78 protein.

5 . The peptide of claim 1 , wherein the peptides represented by the amino acid sequences of SEQ ID NOs: 7 to 14 recognize ATP in the nucleus, which is regulated by GRP78.

6 . The peptide of claim 1 , wherein the peptides represented by the amino acid sequences of SEQ ID NOs: 15 to 18 recognize ATP degradation products in the nucleus, which are regulated by GRP78.

7 . The peptide of claim 1 , wherein the highly efficient stem cells express GRP78.

8 . A method of screening highly efficient stem cells using the peptide of any one of claims 1 to 7 .

9 . The method of claim 8 , comprising steps of:
(a) conjugating a fluorescent to a peptide specific for GRP78; (b) binding the fluorescent-conjugated peptide to stem cells; and (c) screening GRP78-expressing stem cells under a fluorescence microscope.

10 . A composition for screening highly efficient stem cells comprising the peptide of any one of claims 1 to 7 .

11 . A kit for screening highly efficient stem cells comprising the peptide of any one of claims 1 to 7 .

12 . A method of increasing efficiency of stem cells by treating the stem cells with the peptide of any one of claims 1 to 7 .

13 . The method of claim 12 , wherein the stem cells having increased efficiency express GRF78.

14 . A composition for increasing efficiency of stem cells comprising the peptide of any one of claims 1 to 7 .

Assignees

• Nibec Co Ltd
• Nat Univ Chungbuk Ind Acad Coop Found
• Seoul Nat Univ R&Db Foundation

Inventors

• Park Yoon Shin
• Park Yoon Jeong
• Chung Chong-Pyoung
• Lee Jue-Yeon

Classifications

• G01N33/582

  with fluorescent label · CPC title

• G01N33/53

  Immunoassay; Biospecific binding assay; Materials therefor · CPC title

• C07K2319/00

  Fusion polypeptide · CPC title

• C07K9/00

  Peptides having up to 20 amino acids, containing saccharide radicals and having a fully defined sequence; Derivatives thereof · CPC title

• C07K7/08

  having 12 to 20 amino acids (gastrins C07K14/595; somatostatins C07K14/655; melanotropins C07K14/68) · CPC title