Method for identifying exosome surface molecule

Patent metadata

Field	Value
Publication number	US-2020355680-A1
Application number	US-201816640894-A
Country	US
Kind code	A1
Filing date	Aug 28, 2018
Priority date	Aug 29, 2017
Publication date	Nov 12, 2020
Grant date	—

Abstract

The present invention provides a method for identifying an exosome surface molecule, including blocking and washing a carrier having a binding molecule to the exosome surface molecule immobilized thereon with a casein solution or a decomposed casein solution, and mixing a casein solution or a decomposed casein solution and a test sample containing an exosome before contact of the carrier and the test sample.

First claim

1 . A method for identifying an exosome surface molecule, comprising blocking and washing a carrier comprising a binding molecule to the exosome surface molecule immobilized thereon with a casein solution or a decomposed casein solution, and mixing a casein solution or a decomposed casein solution and a test sample containing an exosome before contact of the carrier and the test sample.

2 . A method for identifying an exosome surface molecule, comprising the following steps:
(1) a step of blocking a carrier surface comprising a binding molecule to an exosome surface molecule immobilized thereon with a casein solution or a decomposed casein solution, (2) a step of washing the carrier with a casein solution or a decomposed casein solution, (3) a step of contacting a mixture of a test sample containing an exosome and a casein solution or a decomposed casein solution with the carrier, (4) a step of washing the carrier with a casein solution or a decomposed casein solution, and (5) a step of detecting binding of the exosome surface molecule and the binding molecule.

3 . The method according to claim 1 , wherein the binding between the exosome surface molecule and the binding molecule is detected by an immunological method or a surface plasmon resonance method.

4 . The method according to claim 1 , wherein the binding molecule is an antibody, a cell adhesion factor, lectin or an aptamer.

5 . A mobile phase comprising casein or decomposed casein for identifying an exosome surface molecule by a surface plasmon resonance method.

6 . An apparatus for identifying an exosome surface molecule for practicing the method according to claim 1 .

Assignees

• Horiba Ltd

Inventors

• Irikura Daisuke
• Nakayama Hideki

Classifications

• G01N33/54393 Primary

  Improving reaction conditions or stability, e.g. by coating or irradiation of surface, by reduction of non-specific binding, by promotion of specific binding · CPC title

• C07K14/705

  Receptors; Cell surface antigens; Cell surface determinants {(tumour specific antigens C07K14/4748)} · CPC title

• C07K16/00

  Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies · CPC title

• C12M1/00

  Apparatus for enzymology or microbiology · CPC title

• C12N15/115

  Aptamers, i.e. nucleic acids binding a target molecule specifically and with high affinity without hybridising therewith {; Nucleic acids binding to non-nucleic acids, e.g. aptamers} · CPC title