Near infrared fluorescent biosensors for steroid hormones

Patent metadata

Field	Value
Publication number	US-2020348291-A1
Application number	US-202016866091-A
Country	US
Kind code	A1
Filing date	May 4, 2020
Priority date	May 5, 2019
Publication date	Nov 5, 2020
Grant date	—

Abstract

Corona Phase Molecular Recognition (CoPhMoRe) utilizing a template heteropolymer adsorbed onto and templated by a nanoparticle surface to recognize a specific target analyte can be used for macromolecular analytes, including proteins.

First claim

What is claimed is:

1 . A composition, comprising:
a complex, wherein the complex includes:
a nanostructure; and
a polymer, the polymer adsorbed on the nanostructure and the polymer being free from selective binding to an analyte in the absence of being adsorbed on the nanostructure, wherein the polymer is a heteropolymer including a hydrophobic region and a hydrophilic region and the polymer includes a template moiety; and
a selective binding site associated with the complex, the selectivity of the selective binding site being dependent on the template moiety.

2 . The composition of claim 1 , wherein the nanostructure is a photoluminescent nanostructure.

3 . The composition of claim 2 , wherein the photoluminescent nanostructure is a nanotube, a carbon nanotube, or a single-walled carbon nanotube.

4 . The composition of claim 1 , wherein the polymer includes a polystyrene.

5 . The composition of claim 4 , wherein the polymer includes a polyacrylate.

6 . The composition of claim 1 , wherein the polymer is a polyacrylate-polystyrene copolymer.

7 . The composition of claim 1 , wherein the polymer is covalently bonded to one or more of the template moiety.

8 . The composition of claim 8 , wherein the template moiety includes a steroid moiety.

9 . The composition of claim 1 , wherein the polymer has the structure:

wherein n is 1 to 500, m is an integer from 0 to 500, and k is an integer from 1 to 500;
T is a template moiety;
A is a hydrophilic moiety; and
Q is a group that associates with the nanostructure.

10 . The composition of claim 9 , wherein Q is an aromatic group.

11 . The composition of claim 1 , wherein the template is a cortisol, testosterone, dehydroepiandrosterone (DHEA), pregnenolone, estradiol, prednisone, corticosterone, progesterone, cortisone, aldosterone or prednisolone moiety.

12 . The composition of claim 1 , wherein the analyte is cortisol, testosterone, dehydroepiandrosterone (DHEA), pregnenolone, estradiol, prednisone, corticosterone, progesterone, cortisone, aldosterone or prednisolone.

13 . A sensor including a matrix and the composition of claim 1 suspended in the matrix.

14 . A method for analyzing a sample for an analyte, comprising:
providing a composition of claim 1 ; exposing the composition to a sample; monitoring a property of the composition; and determining a presence of an analyte in the sample based on the property.

15 . The method of claim 14 , wherein the sample includes a gas, a liquid or a solid.

16 . The method of claim 14 , wherein the sample is a biological fluid.

17 . The method of claim 14 , wherein the property is an emission, emission intensity, or an emission wavelength.

18 . The method of claim 14 , wherein exposing the composition to a sample includes inserting the composition into an animal, a plant, or a fungus.

19 . The method of claim 14 , wherein exposing the composition to a sample includes incubating the composition with a microorganism, a virus, a cell line, or an in vitro model system.

20 . The method of claim 14 , wherein determining the presence of an analyte includes determining the absence of the analyte, or determining the concentration of the analyte.

21 . The method of claim 14 , wherein monitoring a property of the composition includes creating an image.

22 . A method of making a composition including:
templating a composition that selective binds an analyte.

23 . The method of claim 22 , wherein templating includes contacting a nanostructure, and a polymer, the polymer adsorbed on the nanostructure and the polymer being free from selective binding to an analyte in the absence of being adsorbed on the nanostructure, wherein the polymer is a heteropolymer including a hydrophobic region and a hydrophilic region and the polymer includes a template moiety.

24 . A system comprising:
the composition claim 1 ; an electromagnetic radiation source having an excitation wavelength directed at the composition; and a detector configured to receive an emission wavelength from the composition.

Assignees

• Massachusetts Inst Technology

Inventors

• Strano Michael
• Lee Michael
• Bakh Naveed
• Wang Song

Classifications

• G01N33/54393

  Improving reaction conditions or stability, e.g. by coating or irradiation of surface, by reduction of non-specific binding, by promotion of specific binding · CPC title

• A61K49/0065

  the luminescent/fluorescent agent having itself a special physical form, e.g. gold nanoparticle · CPC title

• A61B2562/0238

  Optical sensor arrangements for performing transmission measurements on body tissue · CPC title

• A61B5/1459

  invasive, e.g. introduced into the body by a catheter · CPC title

• G01N33/743

  Steroid hormones · CPC title