Carrying module, nucleic acid loading device and use thereof

Patent metadata

Field	Value
Publication number	US-2020338564-A1
Application number	US-201816960748-A
Country	US
Kind code	A1
Filing date	Dec 12, 2018
Priority date	Jan 23, 2018
Publication date	Oct 29, 2020
Grant date	—

Abstract

A nucleic acid loading device, including a housing, a carrying module, a motion module, a liquid circuit module and a control module. The motion module includes a support platform, first and second driving mechanisms, and a carrying member. The liquid circuit module is configured to control a reagent to flow into and out of a reactor. The control module is connected with the motion module and the liquid circuit module, and the control module is configured to control the first and second driving mechanisms, the liquid circuit module and the temperature control unit. A first face of a semiconductor cooler heats or refrigerates during operation, providing heat for an accommodating seat through a heat conducting body such that the reactor can be at different ambient temperatures. The cooperation between the motion and liquid circuit modules may achieve automation and industrialization of loading a sample into the reactor.

First claim

1 . A nucleic acid loading device, comprising:
a carrying module comprising an accommodating seat and a temperature control unit, the accommodating seat being configured to accommodate a reactor, the nucleic acid loading being performed in the reactor, the temperature control unit comprising a semiconductor cooler, a heat conducting body and a temperature sensor, the semiconductor cooler comprising a first face and a second face opposite to each other, the heat conducting body being connected to the first face and the accommodating seat, the temperature sensor being arranged in the heat conducting body, and the temperature sensor being configured to detect a temperature of the heat conducting body; a motion module comprising a partition plate, a support platform, a first driving mechanism, a carrying member and a second driving mechanism, the support platform being detachably and movably arranged on the partition plate, the first driving mechanism being fixed on the partition plate and configured to drive the support platform to move along a first direction relative to the partition plate, the second driving mechanism being fixed on the support platform and configured to drive the carrying member to move along a second direction relative to the support platform, the second direction being perpendicular to the first direction; a liquid circuit module configured to control a reagent to flow into and out of the reactor, and comprising an injection pump configured to provide negative pressure; and a control module connected with the carrying module, the motion module and the liquid circuit module, and configured to control operation of the first driving mechanism, the second driving mechanism, the liquid circuit module and the temperature control unit.

2 . The nucleic acid loading device as claimed in claim 1 , wherein the temperature sensor is a calibrated temperature sensor, and a temperature y detected by the temperature sensor satisfies a polynomial equation

y
=

∑

i
=
0

n



a
i



x
i

,

wherein
n is a natural number and satisfies 4≤n≤6,
x is a temperature detected by the temperature sensor before calibration,
a i is a polynomial coefficient.

3 . The nucleic acid loading device as claimed in claim 1 , wherein the control module comprises a machine-executable program, executing the machine-executable program comprises calibrating the temperature sensor by using the following polynomial equation, and the temperature y detected by the calibrated temperature sensor meets the

y
=

∑

i
=
0

n



a
i



x
i

,

wherein:
n is a natural number and satisfies 4≤n≤6,
x is a temperature detected by the temperature sensor before calibration, as is a polynomial coefficient.

4 . The nucleic acid loading device as claimed in claim 1 , wherein the first driving mechanism comprises a first electric motor fixed on the partition plate and a first transmission shaft connected with the first electric motor, the first transmission shaft is connected with the support platform, and the first electric motor is configured to drive the first transmission shaft to move so as to drive the support platform to move relative to the partition plate.

5 . The nucleic acid loading device as claimed in claim 1 , wherein the second driving mechanism comprises a second electric motor fixed on the support platform and a second transmission shaft connected with the second electric motor, the carrying member is fitted over the second transmission shaft, and the second electric motor is configured to drive the second transmission shaft to rotate so as to drive the carrying member to move along the second direction.

6 . The nucleic acid loading device as claimed in claim 1 , comprising a housing, wherein the housing comprises a panel provided with a window corresponding to the carrying module, and the window is configured to place the reactor on the accommodating seat.

7 - 17 . (canceled)

18 . The nucleic acid loading device as claimed in claim 1 ,
wherein the support platform is configured to connect a reagent kit, the reagent kit being connected with the reactor.

19 . (canceled)

20 . The nucleic acid loading device as claimed in claim 18 , wherein
the carrying member is configured to carry the reagent kit.

21 . The nucleic acid loading device as claimed in claim 20 , wherein the second driving mechanism comprises a second electric motor fixed on the support platform and a second transmission shaft connected with the second electric motor, the carrying member is fitted over the second transmission shaft, and the second electric motor is configured to drive the second transmission shaft to rotate so as to drive the carrying member to move along the second direction.

22 . The nucleic acid loading device as claimed in claim 20 , wherein the injection pump is configured to provide negative pressure for the reactor to allow a reagent in the reagent kit to flow to the reactor.

23 . A method of nucleic acid immobilization and/or hybridization, including a step of immobilizing and/or hybridizing utilizing the nucleic acid loading device as claimed in claim 1 .

24 . The nucleic acid loading device as claimed in claim 1 , wherein the temperature control unit further comprises a heat dissipating body connected with the second face.

25 . The nucleic acid loading device as claimed in claim 24 , wherein the heat dissipating body is formed with a plurality of heat dissipating fins arranged at intervals.

26 . The nucleic acid loading device as claimed in claim 25 , wherein the plurality of heat dissipating fins define a heat dissipating channel configured for cooling liquid to flow through, the carrying module comprises a cover plate, the cover plate covers the heat dissipating channel, and the cover plate is sealedly connected with the heat dissipating body.

27 . The nucleic acid loading device as claimed in claim 26 , wherein the heat dissipating channel is in a meandering shape.

28 . The nucleic acid loading device as claimed in claim 26 , wherein the carrying module comprises an insulating body fixedly connected with the cover plate and the heat conducting body.

29 . The nucleic acid loading device as claimed in claim 1 , wherein the carrying

Assignees

• Genemind Biosciences Co Ltd

Inventors

• Zhang Songzhen
• Jiang Zefei
• Wang Guangming
• Wu Ping
• Zhou Zhiliang
• Yan Qin

Classifications

• C12Q1/6837 Primary

  using probe arrays or probe chips (C12Q1/6874 takes precedence) · CPC title

• B01L7/52 Primary

  with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples · CPC title

• B01L2400/049

  vacuum · CPC title

• B01L3/50273

  characterised by the means or forces applied to move the fluids · CPC title

• B01L2300/1894

  Cooling means; Cryo cooling · CPC title