Stable liquid inoculant compositions and coated plant propagation materials comprising same

Patent metadata

Field	Value
Publication number	US-2020323198-A1
Application number	US-201716305126-A
Country	US
Kind code	A1
Filing date	May 30, 2017
Priority date	May 31, 2016
Publication date	Oct 15, 2020
Grant date	—

Abstract

The present disclosure provides inoculant compositions and methods for enhancing the survival and/or stability of microbial spores in an inoculant composition. In some embodiments, inoculant compositions of the present disclosure comprise microbial spores in a carrier consisting essentially of one or more water-miscible anhydrous liquids, such as cyclohexanol, cyclohexanone, N-methyldiethanolamine, and/or N-methyl-2-pyrrolidone.

First claim

1 - 25 . (canceled)

26 . A liquid inoculant composition, comprising microbial spores in an agriculturally acceptable carrier that comprises a water-miscible, anhydrous liquid selected from the group consisting of cyclohexanol, cyclohexanone, N-methyldiethanolamine (MDEA) and N-methyl-2-pyrrolidone (NMP).

27 . The inoculant composition of claim 26 , wherein said microbial spores comprise about 5 to about 20% (by weight) of said inoculant composition.

28 . The inoculant composition of claim 26 , wherein said microbial spores are present at a concentration of about 1×10 1 to about 1×10 15 colony-forming units per gram of said inoculant composition.

29 . The inoculant composition of claim 26 , wherein said water-miscible, anhydrous liquid comprises about 80 to about 95% (by weight) of said inoculant composition.

30 . The inoculant composition of claim 26 , further comprising one or more dispersants.

31 . The inoculant composition of claim 30 , wherein said one or more dispersants comprise about 0.1 to about 5% (by weight) of said composition.

32 . The inoculant composition of claim 26 , further comprising one or more pesticides.

33 . The inoculant composition of claim 26 , further comprising one or more lipo-chitooligosaccharides.

34 . The inoculant composition of claim 26 , wherein at least 50% of said microbial spores remain viable after said inoculant composition is stored at 20° C. and 30% relative humidity for a period of 12 weeks.

35 . The inoculant composition of claim 26 , wherein at least 50% of said microbial spores exist as single isolated spores when said inoculant composition is diluted 1:10 in water.

36 . A coated plant seed, comprising a plant seed and a coating that covers at least a portion of an outer surface of said seed, said coating comprising the inoculant composition of claim 26 .

37 . A method, comprising applying the inoculation composition of claim 26 to a plant propagation material and/or to a plant that grows from said plant propagation material.

38 . A method, comprising introducing the inoculation composition of claim 26 into a plant growth medium.

Assignees

• Novozymes Bioag As
• Novozymes As

Inventors

• Clary Dan
• Doughan Ben

Classifications

• C12N3/00

  Spore forming or isolating processes · CPC title

• C12N1/14

  Fungi (culture of mushrooms A01G18/00; as new plants A01H15/00); Culture media therefor · CPC title

• C12N1/04

  Preserving or maintaining viable microorganisms (immobilised microorganisms C12N11/00) · CPC title

• A01N25/26

  in coated particulate form · CPC title

• A01N25/04 Primary

  Dispersions, {emulsions, suspoemulsions, suspension concentrates} or gels (foams A01N25/16) · CPC title