Mesenchymal stem cells (MSC) expansion methods and materials
US-9220810-B2 · Dec 29, 2015 · US
US2020291358A1 · US · A1
| Field | Value |
|---|---|
| Publication number | US-2020291358-A1 |
| Application number | US-201816645213-A |
| Country | US |
| Kind code | A1 |
| Filing date | Sep 6, 2018 |
| Priority date | Sep 8, 2017 |
| Publication date | Sep 17, 2020 |
| Grant date | — |
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An object of the present invention is to provide stem cells having excellent proliferation ability and differentiation ability. The present invention relates to stem cells isolated from a neonatal pig, and a preparation method therefor.
Opening claim text (preview).
1 . Stem cells isolated from a neonatal pig. 2 . The stem cells according to claim 1 , wherein an average diameter is 17 μm or less. 3 . The stem cells according to claim 1 , wherein a doubling time in a logarithmic growth phase is 36 hours or less. 4 . The stem cells according to claim 1 , wherein the stem cells are mesenchymal stem cells. 5 . The stem cells according to claim 1 , wherein the neonatal pig is a neonatal pig that enables cell transplantation into a human. 6 . The stem cells according to claim 1 , wherein the stem cells are isolated from the bone marrow or the pancreatic islet of the neonatal pig. 7 . The stem cells according to claim 1 , wherein the stem cells are stem cells for transplantation. 8 . The stem cells according to claim 7 , wherein the stem cells are stem cells for human transplantation. 9 . The stem cells according to claim 1 , wherein the stem cells are stem cells for feeder cells. 10 . The stem cells according to claim 9 , wherein the stem cells are stem cells for feeder cells for proliferating stem cells for human transplantation. 11 . A method for preparing stem cells, comprising a step of isolating cells from a neonatal pig. 12 . The method for preparing stem cells according to claim 11 , comprising a step of subculturing the stem cells after 3 to 12 days from inoculation. 13 . The method for preparing stem cells according to claim 11 , wherein the cells to be isolated from the neonatal pig are cells in a monocytic cell fraction. 14 . The method for preparing stem cells according to claim 11 , wherein the isolation of the cells from the neonatal pig is isolation of the cells from the bone marrow or the pancreatic islet of the neonatal pig. 15 . The method for preparing stem cells according to claim 13 , comprising a step of freezing the isolated cells in the monocytic cell fraction.
Temperature processes, e.g. following predefined temperature changes over time · CPC title
Cells from the blood or the immune system · CPC title
Pancreatic cells · CPC title
Bone marrow mesenchymal stem cells (BM-MSC) · CPC title
Collagen; Gelatin · CPC title
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