Immunosuppressive protein adsorption material and adsorption column

Patent metadata

Field	Value
Publication number	US-2020246776-A1
Application number	US-201816638586-A
Country	US
Kind code	A1
Filing date	Sep 7, 2018
Priority date	Sep 8, 2017
Publication date	Aug 6, 2020
Grant date	—

Abstract

An objective of the present disclosure is to provide an adsorption material that has a carrier material with retained physical strength, and efficiently adsorbs an immunosuppressive protein. The present disclosure provides an adsorption material for immunosuppressive protein. The adsorption material includes a water-insoluble carrier to which at least one nitrogen-containing compound selected from a polyamine represented by a predetermined formula and aliphatic amines represented by predetermined formulae is bound. A total content of amino groups on the water-insoluble carrier is more than 0 μmol and 2500 μmol or less per 1 g, and a content of primary amino groups on the water-insoluble carrier is 450 μmol or less per 1 g.

First claim

1 . An adsorption material for immunosuppressive protein, the adsorption material comprising:
a water-insoluble carrier to which at least one nitrogen-containing compound is bound, the nitrogen-containing compound being selected from a polyamine represented by the following Formula (1), a primary aliphatic amine represented by the following Formula (2), and a secondary aliphatic amine represented by the following Formula (3), wherein a total content of amino groups on the water-insoluble carrier is more than 0 μmol and 2500 μmol or less per 1 g, and a content of primary amino groups on the water-insoluble carrier is 450 μmol or less per 1 g:
R 1 R 2 N—X—NR 3 R 4   Formula (1)

wherein in Formula (1), X is a saturated or unsaturated aliphatic hydrocarbon group having 2 to 20 carbon atoms, or a heteroatom-containing carbon chain in which 1 to 5 carbon atoms of a saturated or unsaturated aliphatic hydrocarbon group having 3 to 20 carbon atoms are replaced with a nitrogen atom, a hydrogen atom that bonds to the nitrogen atom may be replaced with an alkyl group that may have an amino group, and R 1 to R 4 are each independently a hydrogen atom or an alkyl group;
NH 2 R 5   Formula (2)

wherein in Formula (2), R 5 is a saturated or unsaturated aliphatic hydrocarbon group having 1 to 12 carbon atoms;
NHR 6 R 7   Formula (3)

wherein in Formula (3), R 6 and R 7 are each independently a saturated or unsaturated aliphatic hydrocarbon group having 1 to 12 carbon atoms.

2 . The adsorption material according to claim 1 ,
wherein the nitrogen-containing compound comprises the polyamine represented by the Formula (1).

3 . The adsorption material according to claim 1 ,
wherein the nitrogen-containing compound binds to the water-insoluble carrier via a linker.

4 . The adsorption material according to claim 1 ,
wherein the total content of amino groups is 30 to 2400 μmol per 1 g.

5 . The adsorption material according to claim 4 ,
wherein a proportion of the content of primary amino groups to the total content of amino groups (the content of primary amino groups/the total content of amino groups) is 0.30 or less.

6 . The adsorption material according to claim 1 ,
wherein the water-insoluble carrier has a form of fiber or particle, wherein the fiber or the particle has a diameter of 15 to 50 μm, and wherein the water-insoluble carrier has a surface with an arithmetic mean roughness of 0.1 to 3.0 μm.

7 . The adsorption material according to claim 1 ,
wherein the immunosuppressive protein is a TGF-β or a LAP-bound TGF-β.

8 . An adsorption column comprising the adsorption material according to claim 1 .

9 . The adsorption column according to claim 8 ,
wherein the adsorption column is used for a blood purification therapy.

10 . The adsorption material according to claim 2 ,
wherein the nitrogen-containing compound binds to the water-insoluble carrier via a linker.

11 . The adsorption material according to claim 2 ,
wherein the total content of amino groups is 30 to 2400 μmol per 1 g.

12 . The adsorption material according to claim 3 ,
wherein the total content of amino groups is 30 to 2400 μmol per 1 g.

13 . The adsorption material according to claim 2 ,
wherein the water-insoluble carrier has a form of fiber or particle, wherein the fiber or the particle has a diameter of 15 to 50 μm, and wherein the water-insoluble carrier has a surface with an arithmetic mean roughness of 0.1 to 3.0 μm.

14 . The adsorption material according to claim 3 ,
wherein the water-insoluble carrier has a form of fiber or particle, wherein the fiber or the particle has a diameter of 15 to 50 μm, and wherein the water-insoluble carrier has a surface with an arithmetic mean roughness of 0.1 to 3.0 μm.

15 . The adsorption material according to claim 4 ,
wherein the water-insoluble carrier has a form of fiber or particle, wherein the fiber or the particle has a diameter of 15 to 50 μm, and wherein the water-insoluble carrier has a surface with an arithmetic mean roughness of 0.1 to 3.0 μm.

16 . The adsorption material according to claim 5 ,
wherein the water-insoluble carrier has a form of fiber or particle, wherein the fiber or the particle has a diameter of 15 to 50 μm, and wherein the water-insoluble carrier has a surface with an arithmetic mean roughness of 0.1 to 3.0 μm.

17 . The adsorption material according to claim 2 ,
wherein the immunosuppressive protein is a TGF-β or a LAP-bound TGF-β.

18 . The adsorption material according to claim 3 ,
wherein the immunosuppressive protein is a TGF-β or a LAP-bound TGF-β.

19 . The adsorption material according to claim 4 ,
wherein the immunosuppressive protein is a TGF-β or a LAP-bound TGF-β.

20 . The adsorption material according to claim 5 ,
wherein the immunosuppressive protein is a TGF-β or a LAP-bound TGF-β.

Assignees

• Toray Industries
• National Univ Corporation Shiga Univ Of Medical Science

Inventors

• Kasuya Junichi
• MATSUNAGA Ryo
• Ueno Yoshiyuki
• Ueda Yuji
• Teramoto Kazuo
• Ogasawara Kazumasa

Classifications

• A61M1/362

  changing physical properties of target cells by binding them to added particles to facilitate their subsequent separation from other cells, e.g. immunoaffinity · CPC title

• B01J20/28

  characterised by their form or physical properties · CPC title

• A61M1/3679 Primary

  by absorption (A61M1/3675 takes precedence) · CPC title

• B01J20/26 Primary

  Synthetic macromolecular compounds · CPC title

• A61M1/3678

  Separation of cells using wave pressure; Manipulation of individual corpuscles · CPC title