NOXA-DERIVED, CELL DEATH-INDUCING PEPTIDE eMTD

Patent metadata

Field	Value
Publication number	US-2020239521-A1
Application number	US-202016774423-A
Country	US
Kind code	A1
Filing date	Jan 28, 2020
Priority date	Jan 28, 2019
Publication date	Jul 30, 2020
Grant date	—

Abstract

Disclosed herein is a cell death-inducing peptide that rapidly acts. The peptide is derived from Noxa protein and comprises 16 amino acid residues including MTD. The peptide is designated extended MTD (eMTD) because it contains the 10-mer MTD. eMTD rapidly exhibits potent necrotic cell death in various cell lines and, as such, can be applied to the treatment of various diseases including cancer when used in conjugation with peptides or materials for targeting specific cells.

First claim

What is claimed is:

1 . A peptide consisting of the amino acid sequence of SEQ ID NO: 1 or 2.

2 . The peptide of claim 1 , wherein the peptide further comprising a tumor-homing peptide (THP) conjugated to the N- or c-terminal of the peptide.

3 . The peptide of claim 2 , wherein the tumor-homing peptide (THP) comprises an amino acid sequence selected from SEQ ID NOS: 5 to 31.

4 . A polynucleotide coding for the peptide of any one of claims 1 to 3 .

5 . A recombinant vector carrying the polynucleotide of claim 4 .

6 . A transformed cell transformed with the recombinant vector of claim 5 .

7 . A composition comprising the peptide of any one of claims 1 to 3 .

8 . Method for prevention or treatment of cancer comprising:
administering to a subject a composition comprising the peptide of any one of claims 1 to 3 .

9 . The method of claim 8 , wherein the cancer is lung cancer, breast cancer, liver cancer, melanoma, stomach cancer, pancreatic cancer, colorectal cancer, ovarian cancer, renal cell carcinoma, prostate cancer, or brain tumor.

Assignees

• Univ Chosun Iacf

Inventors

• Kim Tae-Hyoung
• Park Junghee
• Han Ji-Hye
• Myung Seung-Hyun

Classifications

• C07K2319/00

  Fusion polypeptide · CPC title

• A61P35/00 Primary

  Antineoplastic agents · CPC title

• A61K38/00

  Medicinal preparations containing peptides (peptides containing beta-lactam rings A61K31/00; cyclic dipeptides not having in their molecule any other peptide link than those which form their ring, e.g. piperazine-2,5-diones, A61K31/00; ergot alkaloids of the cyclic peptide type A61K31/48; containing macromolecular compounds having statistically distributed amino acid units A61K31/74; medicinal preparations containing antigens or antibodies A61K39/00; medicinal preparations characterised by the non-active ingredients, e.g. peptides as drug carriers, A61K47/00) · CPC title

• C07K7/08 Primary

  having 12 to 20 amino acids (gastrins C07K14/595; somatostatins C07K14/655; melanotropins C07K14/68) · CPC title

• C12N15/62

  DNA sequences coding for fusion proteins · CPC title