Methods for controlling the growth of prokaryotic and eukaryotic cells

Patent metadata

Field	Value
Publication number	US-2020224146-A1
Application number	US-202016836664-A
Country	US
Kind code	A1
Filing date	Mar 31, 2020
Priority date	Mar 29, 2018
Publication date	Jul 16, 2020
Grant date	—

Abstract

The present disclosure relates to methods for control of cell growth rates where cell growth is measured in situ. The methods are applicable to bacterial cells, mammalian cells, non-mammalian eukaryotic cells, plant cells, yeast cells, fungi, and archea.

First claim

We claim:

1 . A cell growth device comprising:
a housing; a motor; a thermal control device; a processor; an electrical connection configured to be electrically coupled to the thermal control device; and a rotating growth vial comprising a vial comprising two or more paddles extending into an interior of the vial from an interior surface of an outer wall of the vial; and a drive engagement mechanism configured to engage with the motor to spin the vial; wherein the processor accepts input from a user and through the electrical connection directs the thermal control device to adjust the temperature of the vial to grow the cells.

2 . The cell growth device of claim 1 , wherein the vial volume is 1-250 ml.

3 . The cell growth device of claim 2 , wherein the vial volume is 2-100 ml.

4 . The cell growth device of claim 3 , wherein the vial volume is 12-35 ml.

5 . The cell growth device of claim 1 , wherein the vial is fabricated from polycarbonate or polypropylene.

6 . The cell growth device of claim 5 , wherein the vial is fabricated by injection molding.

7 . The cell growth device of claim 1 , further comprising a spectrophotometer, wherein the vial further comprises a light path through the vial for a light beam generated by the spectrophotometer, and wherein the spectrophotometer is configured to measure and deliver to the processor an optical density of cells in the vial.

8 . The cell growth device of claim 7 , wherein spectrophotometer measures the optical density of the cells in the vial and through the electrical connection directs the thermal control device to adjust the temperature of the vial to grow the cells to a target value at a target time.

9 . The cell growth device of claim 1 , wherein the rotating growth vial comprises 3, 4, 5, or 6 paddles.

10 . The cell growth device of claim 9 , wherein the width of the paddles may range from 1/20 to just under ½ the diameter of the rotating growth vial.

11 . A cell growth device comprising:
a housing; a motor; a thermal control device; a processor; an electrical connection configured to be electrically coupled to the thermal control device; and a rotating growth vial comprising a vial comprising two or more paddles that extend from a center of the rotating growth vial toward an inner surface of an outer wall of the rotating growth vial; and a drive engagement mechanism configured to engage with the motor to spin the vial; and wherein the processor accepts input from a user, and through the electrical connection directs the thermal control device to adjust the temperature of the vial to grow the cells.

12 . The cell growth device of claim 11 , wherein the vial volume is 1-250 ml.

13 . The cell growth device of claim 12 , wherein the vial volume is 2-100 ml.

14 . The cell growth device of claim 11 , wherein the width of the paddles may range from 1/20 to just under ½ the diameter of the rotating growth vial.

15 . The cell growth device of claim 11 , wherein the vial is fabricated from polycarbonate or polypropylene.

16 . The cell growth device of claim 15 , wherein the vial is fabricated by injection molding.

17 . The cell growth device of claim 16 , further comprising a spectrophotometer, wherein the vial further comprises a light path through the vial for a light beam generated by the spectrophotometer, and wherein the spectrophotometer is configured to measure and deliver to the processor an optical density of cells in the vial.

18 . The cell growth device of claim 17 , wherein spectrophotometer measures the optical density of the cells in the vial and through the electrical connection directs the thermal control device to adjust the temperature of the vial to grow the cells to a target value at a target time.

19 . The cell growth device of claim 17 , wherein the vial comprises a second light path.

20 . The cell growth device of claim 11 , wherein the rotating growth vial comprises 3, 4, 5, or 6 paddles.

Assignees

• Inscripta Inc

Inventors

• Belgrader Phillip
• Masquelier Don

Classifications

• C12N15/87

  Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation · CPC title

• C12M41/48

  Automatic or computerized control (automatic analysis G01N35/00) · CPC title

• C12M35/08

  Chemical, biochemical or biological means, e.g. plasma jet, co-culture · CPC title

• C12M35/04 Primary

  Mechanical means, e.g. sonic waves, stretching forces, pressure or shear stimuli · CPC title

• C12M23/08

  Flask, bottle or test tube · CPC title