3d printed scaffolds of peptide conjugate polymer

Patent metadata

Field	Value
Publication number	US-2020206384-A1
Application number	US-201816632992-A
Country	US
Kind code	A1
Filing date	Jul 23, 2018
Priority date	Jul 23, 2017
Publication date	Jul 2, 2020
Grant date	—

Abstract

Described herein is a 3D-printed scaffold comprising a peptide-polymer conjugate, the peptide-polymer conjugate having the structure: X-Y-Z-Y-X, wherein X is a biologically active peptide, Y is a linker moiety, and Z is a biocompatible and biodegradable polymer.

First claim

1 . A 3D-printed scaffold comprising a peptide-polymer conjugate, the peptide-polymer conjugate having the structure:
X-Y-Z-Y-X
wherein X is a biologically active peptide, Y is a linker moiety, and Z is a biocompatible and polymer.

2 . The 3D-printed scaffold of claim 1 , wherein Z is poly(caprolactone).

3 . The 3D-printed scaffold of claim 2 , wherein the poly(caprolactone) has a molecular weight ranging from about 10,000 to about 18,000 g/mol.

4 . (canceled)

5 . The 3D-printed scaffold according to claim 1 , further comprising a high molecular weight polymer blended with the peptide-polymer conjugate.

6 . The 3D-printed scaffold according to claim 5 , wherein the high molecular weight polymer comprises poly(caprolactone) homopolymer.

7 . (canceled)

8 . The 3D-printed scaffold according to claim 1 , wherein Y is formed with a maleimide functionalized compound.

9 . The 3D-printed scaffold according to claim 8 , wherein the maleimide functionalized compound comprises an isocyanate group.

10 . The 3D-printed scaffold according to claim 8 , wherein Y is formed from p-maleimidophenyl isocyanate.

11 . The 3D-printed scaffold according to claim 1 , wherein the biologically active peptide is formed with a thiol-functionalized peptide.

12 . The 3D-printed scaffold according to claim 11 , wherein the thiol-functionalized peptide is a cysteine containing peptide.

13 . The 3D-printed scaffold according to claim 1 , wherein the biologically active peptide comprises about 5 to about 30 amino acids.

14 . An implantable scaffold comprising:
a first layer comprising a plurality of first fibers oriented in a first direction; and a second layer comprising a plurality of second fibers oriented in a second direction; wherein the first direction and the second direction are different and the pluralities of first and second fibers comprise peptide-polymer conjugate.

15 . The implantable scaffold according to claim 14 , wherein the peptide-polymer conjugate comprises poly(caprolactone) block and terminal peptide blocks.

16 - 17 . (canceled)

18 . The implantable scaffold according to claim 14 , wherein each of the plurality of first fibers have substantially the same diameter.

19 . (canceled)

20 . The implantable scaffold according to claim 14 , wherein each of the plurality of second fibers have substantially the same diameter.

21 . (canceled)

22 . The implantable scaffold according to claim 14 , wherein the first direction intersects the second direction to form an intersection angle, whereby the intersection angle is a right angle, or an oblique angle.

23 . (canceled)

24 . The implantable scaffold according to claim 14 , further comprising a third layer formed of a plurality of third fibers oriented in a third direction, wherein the third direction is not parallel to the first direction or the second direction.

25 . (canceled)

26 . The implantable scaffold according to claim 24 , wherein the implantable scaffold comprising at least fourteen layers, each comprising a corresponding plurality of fibers.

27 . A method of forming an implantable scaffold comprising:
a) 3D-printing a plurality of first fibers in a first direction to form a first layer; b) 3D-printing a plurality of second fibers in a second direction atop the first layer to form a second layer, wherein the first and second directions are different; wherein the first and second fibers comprise peptide-polymer conjugate.

28 . The method according to claim 27 , wherein the 3D-printing of step a) includes 3D-printing a first ink composition to form the plurality of first fibers, the first ink composition comprising a volatile solvent and the peptide-polymer conjugate; and wherein the 3D-printing of step b) includes 3D-printing a second ink composition to form the plurality of second fibers, the second ink composition comprising a volatile solvent and the peptide-polymer conjugate.

29 - 34 . (canceled)

Assignees

• Univ Lehigh

Inventors

• Chow Lesley W
• Dailey Hannah L

Classifications

• A61L27/54 Primary

  Biologically active materials, e.g. therapeutic substances {(A61L27/227 takes precedence)} · CPC title

• A61L27/18 Primary

  obtained otherwise than by reactions only involving carbon-to-carbon unsaturated bonds · CPC title

• B33Y70/00

  Materials specially adapted for additive manufacturing · CPC title

• A61L27/26

  Mixtures of macromolecular compounds · CPC title

• B33Y10/00

  Processes of additive manufacturing · CPC title