Methods for controlling the growth of prokaryotic and eukaryotic cells

Patent metadata

Field	Value
Publication number	US-2020172846-A1
Application number	US-202016780640-A
Country	US
Kind code	A1
Filing date	Feb 3, 2020
Priority date	Mar 29, 2018
Publication date	Jun 4, 2020
Grant date	—

Abstract

The present disclosure relates to methods for control of cell growth rates where cell growth is measured in situ. The methods are applicable to bacterial cells, mammalian cells, non-mammalian eukaryotic cells, plant cells, yeast cells, fungi, and archea.

First claim

We claim:

1 . An automated cell processing system comprising
a cell growth device comprising: a housing; a motor; a thermal control device; a spectrophotometer; a processor; an electrical connection configured to be electrically coupled to the thermal control device; and a rotating growth vial wherein the rotating growth vial comprises a vial; a drive engagement mechanism configured to engage with the motor to spin the vial; and a light path through the vial for a light beam generated by the spectrophotometer, wherein the spectrophotometer is configured to measure and deliver to the processor an optical density of cells in the vial; a cell concentration module; and a transformation module; wherein the processor accepts input from a user, receives from the spectrophotometer the optical density of the cells in the vial, through the electrical connection directs the thermal control device to adjust the temperature of the vial.

2 . The automated cell processing system of claim 1 , wherein optical density is measured at a pre-programmed wavelength.

3 . The automated cell processing system of claim 1 , wherein optical density is measured at a wavelength selected by a user.

4 . The automated cell processing system of claim 1 , wherein the optical density is measured continuously.

5 . The automated cell processing system of claim 1 , wherein the optical density is measured at intervals specified by the user or programmed into the processor.

6 . The automated cell processing system of claim 1 , wherein the processor notifies a user when a desired optical density is reached.

7 . The automated cell processing system of claim 1 , wherein the vial volume is 2-100 ml.

8 . The automated cell processing system of claim 1 , wherein the cells are grown to a target optical density value at a target time.

9 . The automated cell processing system of claim 1 , wherein the vial is fabricated from polycarbonate or polypropylene.

10 . The automated cell processing system of claim 1 , wherein the transformation module comprises a flow-through electroporation device.

11 . An automated cell processing system comprising:
a cell growth device comprising a housing; a motor; a thermal control device; a spectrophotometer; a processor; an electrical connection configured to be electrically coupled to the thermal control device; and a disposable rotating cell growth vial wherein the disposable rotating growth vial comprises a vial; a drive engagement mechanism connected to the motor and configured to spin the vial; and a first light path through the vial to measure an optical density of cells in the vial via the spectrophotometer; a transformation module; and an editing module; wherein the processor accepts input from a user, receives from the spectrophotometer the optical density of the cells, and through the electrical connection directs the thermal device to adjust the temperature of the vial to grow the cells.

12 . The automated cell processing system of claim 11 , wherein the optical density is measured at a pre-programmed wavelength.

13 . The automated cell processing system of claim 11 , wherein the optical density is measured at a wavelength selected by a user.

14 . The automated cell processing system of claim 11 , wherein the optical density is measured continuously.

15 . The automated cell processing system of claim 11 , wherein the optical density is measured at intervals specified by the user or programmed into the processor.

16 . The automated cell processing system of claim 11 , wherein the processor notifies a user when a desired optical density is reached.

17 . The automated cell processing system of claim 11 , wherein the cells are grown to a target optical density value at a target time.

18 . The automated cell processing system of claim 11 , wherein the transformation module comprises a flow-through electroporation device.

19 . The automated cell processing system of claim 11 , further comprising a liquid handling system controlled by the processor.

20 . The automated cell processing system of claim 11 , wherein the vial further comprises a second light path.

Assignees

• Inscripta Inc

Inventors

• Belgrader Phillip
• Masquelier Don

Classifications

• C12M27/02

  Stirrer or mobile mixing elements · CPC title

• C12M35/04 Primary

  Mechanical means, e.g. sonic waves, stretching forces, pressure or shear stimuli · CPC title

• C12M23/08

  Flask, bottle or test tube · CPC title

• C12M35/02

  Electrical or electromagnetic means, e.g. for electroporation or for cell fusion · CPC title

• C12M23/44

  Multiple separable units; Modules · CPC title