Culture device for lactic acid bacteria

Patent metadata

Field	Value
Publication number	US-2020140916-A1
Application number	US-201916715975-A
Country	US
Kind code	A1
Filing date	Dec 16, 2019
Priority date	Apr 29, 2015
Publication date	May 7, 2020
Grant date	—

Abstract

The present disclosure provides a culture device for enumerating colonies of microorganisms. The device can comprise a base, a coversheet, and a nonporous spacer member disposed therebetween. The spacer member comprises an aperture that defines a growth compartment. Disposed in the growth compartment are a cold water-soluble gelling agent, a dry oxygen-scavenging reagent, a dry buffer system, and an effective amount of a dry carbon dioxide-generating reagent. The buffer system is selected such that, when the growth compartment is hydrated with a predetermined volume of deionized water, an aqueous mixture with a pt less than or equal to 6.35 is formed.

First claim

1 . A culture device for enumerating colonies of a target microorganism, the device comprising:
a base having opposing inner and outer surfaces; a coversheet having opposing inner and outer surfaces; a nonporous spacer member disposed between the base and the cover sheet, wherein the spacer member is coupled to the base or the coversheet, wherein the spacer member comprises an aperture that defines a shape and a depth of a growth compartment, wherein the spacer member and the growth compartment are disposed between the inner surface of the base and the inner surface of the coversheet; an effective amount of a dry oxygen-scavenging reagent disposed in the growth compartment; a dry, cold-water-soluble gelling agent disposed in the growth compartment, wherein the gelling agent is adhered to the base or the coversheet in the growth compartment; a dry buffer system disposed in the growth compartment that, when the growth compartment is hydrated with a predetermined volume of deionized water, forms an aqueous mixture with a pH less than or equal to 635; and an effective amount of a dry carbon dioxide-generating reagent disposed in the growth compartment.

Assignees

• 3M Innovative Properties Co

Inventors

• Bjork Jason W
• Brutinel Evan D
• Stanenas Adam J

Classifications

• C12M41/36

  of biomass, e.g. colony counters or by turbidity measurements (electrooptical investigation of individual particles G01N15/14, flow cytometers G01N15/1404) · CPC title

• C12Q1/04 Primary

  Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor {(C12Q1/6897 takes precedence)} · CPC title

• C12M23/20

  Material Coatings (immunocoatings C12M25/00) · CPC title

• C12M41/34

  of gas · CPC title

• C12M23/04 Primary

  Flat or tray type, drawers (C12M23/10, C12M23/12, C12M23/16 take precedence) · CPC title