Multi-Well Plate Adaptors

Patent metadata

Field	Value
Publication number	US-2020122147-A1
Application number	US-201816626138-A
Country	US
Kind code	A1
Filing date	Oct 31, 2018
Priority date	Nov 3, 2017
Publication date	Apr 23, 2020
Grant date	—

Abstract

Disclosed are multi-well plate inserts that can be used to separate solid debris, including paper punch containing a blood sample, from a liquid containing target biological molecules, such as nucleic acid molecules and proteins. Also provided are methods of using the insert, for example as part of a method that analyzes target biological molecules.

First claim

We claim:

1 . A multi-well plate adaptor, comprising:
two or more component parts, each component part comprising:
a top surface with hollow conical protrusions extending therefrom, the axis of the conical protrusions being perpendicular to the top surface; and
each hollow conical protrusion comprising an angled tip at the end distal from the top surface, the angled tip comprising one or more holes, and one or more ridges on an outer surface of the angled tip.

2 . The multi-well plate adaptor of claim 1 , wherein the multi-well plate is a 96-well plate.

3 . The multi-well plate adaptor of claim 2 , comprising three component parts.

4 . The multi-well plate adaptor of claim 3 , wherein the top surface has a length of about 75-95 mm and a width of about 20-30 mm.

5 . The multi-well plate adaptor of claim 1 , wherein a distance that each hollow conical protrusion extends from the top surface is shorter than a depth of the wells of the multi-well plate.

6 . The multi-well plate adaptor of claim 5 , wherein the distance each hollow conical protrusion extends from the top surface is about 15 mm-30 mm.

7 . The multi-well plate adaptor of claim 1 , wherein an inner diameter of the hollow conical protrusions can accommodate a pipette tip.

8 . The multi-well plate adaptor of claim 1 , wherein the one or more holes of the angled tip has a minimal open dimension of about 0.6 mm-0.8 mm.

9 . The multi-well plate adaptor of claim 1 , wherein the angled tip comprises a central hole at the distal end and multiple longitudinal holes radiating symmetrically therefrom, each hole separated by a portion of the angled tip, and wherein the portion of the angled tip separating each of the longitudinal holes further comprises the one or more ridges on the outer surface of the angled tip, wherein the central hole has a diameter of about 0.6 mm-1 mm and the longitudinal holes comprise a minimal open dimension of about 0.6 mm-1 mm.

10 . A well plate insert, comprising:
two or more insert components, each insert component comprising a rectangular top surface with hollow extensions protruding therefrom, wherein a main axis of the hollow extensions is at least substantially perpendicular to a plane of the rectangular top surface;
the hollow extensions having at their terminus a distal tip with one or more perforations;
the distal tip further comprising, on its outer surface, one or more ridges extending away from the main body of the distal tip; and
a long edge of the rectangular top surface adapted to fit with the long edge of the rectangular top surface of another insert component.

11 . The well plate insert of claim 10 , wherein a main body of the hollow extensions is an elongate frusto-conical extension and the distal tip extends substantially conically from a smaller diameter end of the hollow extension.

12 . The well plate insert of claim 11 , wherein the main body of the hollow extensions comprises a solid surface.

13 . The well plate insert of claim 11 , wherein the one or more ridges do not extend beyond the distal tip onto the main body of the hollow extensions.

14 . The well plate insert of claim 10 , wherein the hollow extensions do not comprise a mesh or fiber insert.

15 . The well plate insert of claim 10 , wherein each perforation has a minimal open dimension of about 0.6 mm-0.8 mm.

16 . The well plate insert of claim 10 , wherein the one or more ridges of the distal tip are capable of piercing a seal of the well plate.

17 . The well plate insert of claim 10 , wherein the one or more perforations are sized to allow a lysate to flow through and to prevent passage of a particulate in a well of the well plate.

18 . The well plate insert of claim 10 , wherein each insert component comprises 32 hollow extensions arranged in a grid with four hollow extensions along a short edge of the rectangular top surface and eight hollow extensions along the long edge of the rectangular top surface.

19 . The well plate insert of claim 18 , adapted to nest on top of a 96-well plate with the hollow extensions protruding into each individual well.

20 . A lysate isolation plate adaptor, comprising:
three identical components parts, each comprising;
a planar rectangular main body with 32 elongate extensions protruding in a grid pattern therefrom, wherein a main axis of each elongate extension is at least substantially perpendicular to the planar rectangular main body;
each elongate extension having a rounded tip with a plurality of holes therein, each hole having a minimal open dimension of 0.6 mm-0.8 mm; and
one or more angled spines on an exterior surface of the rounded tip.

Assignees

• Illumina Inc
• Illumina Cambridge Ltd

Inventors

• Joad Andrew
• Fuller Daniel L
• Crivelli Paul

Classifications

• B01L2200/0631

  Purification arrangements, e.g. solid phase extraction [SPE] · CPC title

• B01L3/5085

  for multiple samples, e.g. microtitration plates · CPC title

• C12M23/38

  Caps; Covers; Plugs; Pouring means · CPC title

• B01L2300/0829

  Multi-well plates; Microtitration plates · CPC title

• C12M25/04

  in combination with well or multiwell plates, i.e. culture inserts · CPC title