Compositions for saccharification of cellulosic material
US-2024018560-A1 · Jan 18, 2024 · US
US2020087689A1 · US · A1
| Field | Value |
|---|---|
| Publication number | US-2020087689-A1 |
| Application number | US-201916582629-A |
| Country | US |
| Kind code | A1 |
| Filing date | Sep 25, 2019 |
| Priority date | Mar 31, 2017 |
| Publication date | Mar 19, 2020 |
| Grant date | — |
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Provided are a composition for producing tagatose, comprising fructose-4-epimerase, and a method of producing tagatose using the same.
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What is claimed is: 1 . A composition for producing tagatose, comprising tagatose-6-phosphate kinase, a microorganism expressing the tagatose-6-phosphate kinase, or a culture of the microorganism. 2 . The composition for producing tagatose of claim 1 , further comprising fructose. 3 . The composition for producing tagatose of claim 1 , wherein the composition comprises one or more of tagatose-6-phosphate kinase consisting of an amino acid sequence of SEQ ID NO: 1, 3, 5, 7, 9, or 11. 4 . The composition for producing tagatose of claim 1 , wherein the tagatose-6-phosphate kinase is an enzyme derived from Anaerolineae sp., the genus of Thermobifida , the genus of Thermoanaerobacter , or the genus of Dictyoglomus , or a variant thereof. 5 . A method of producing tagatose, comprising converting fructose into tagatose by contacting fructose with tagatose-6-phosphate kinase, a microorganism expressing the tagatose-6-phosphate kinase, or a culture of the microorganism. 6 . The method of producing tagatose of claim 5 , wherein the contacting is performed under conditions of pH 5.0 to pH 9.0 and 30° C. to 80° C. for 0.5 hours to 48 hours.
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