Plant regulatory elements and uses thereof

Patent metadata

Field	Value
Publication number	US-2020080098-A1
Application number	US-201916563801-A
Country	US
Kind code	A1
Filing date	Sep 6, 2019
Priority date	Mar 11, 2016
Publication date	Mar 12, 2020
Grant date	—

Abstract

The invention provides recombinant DNA molecules and constructs, as well as their nucleotide sequences, useful for modulating gene expression in plants. The invention also provides transgenic plants, plant cells, plant parts, and seeds comprising the recombinant DNA molecules operably linked to heterologous transcribable DNA molecules, as are methods of their use.

First claim

1 . A recombinant DNA molecule comprising a DNA sequence selected from the group consisting of:
a) a sequence with at least 85 percent sequence identity to any of SEQ ID NOs: 7-9; b) a sequence comprising any of SEQ ID NOs: 7-9; and c) a fragment of any of SEQ ID NOs: 7-9, wherein the fragment has gene-regulatory activity;
wherein said sequence is operably linked to a heterologous transcribable DNA molecule.

2 . The recombinant DNA molecule of claim 1 , wherein said sequence has at least 90 percent sequence identity to the DNA sequence of any of SEQ ID NOs: 7-9.

3 . The recombinant DNA molecule of claim 2 , wherein said sequence has at least 95 percent sequence identity to the DNA sequence of any of SEQ ID NOs: 7-9.

4 . The recombinant DNA molecule of claim 3 , wherein said sequence comprises the DNA sequence of any of SEQ ID NOs: 7-9.

5 . The recombinant DNA molecule of claim 1 , wherein the DNA sequence comprises gene regulatory activity.

6 . The recombinant DNA molecule of claim 1 , wherein the heterologous transcribable DNA molecule comprises a gene of agronomic interest.

7 . The recombinant DNA molecule of claim 6 , wherein the gene of agronomic interest confers herbicide tolerance in plants.

8 . The recombinant DNA molecule of claim 6 , wherein the gene of agronomic interest confers pest resistance in plants.

9 . A transgenic plant cell comprising a recombinant DNA molecule comprising a sequence selected from the group consisting of:
a) a sequence with at least 85 percent sequence identity to any of SEQ ID NOs: 7-9; b) a sequence comprising any of SEQ ID NOs: 7-9; and c) a fragment of any of SEQ ID NOs: 7-9, wherein the fragment has gene-regulatory activity;
wherein said sequence is operably linked to a heterologous transcribable DNA molecule.

10 . The transgenic plant cell of claim 9 , wherein said transgenic plant cell is a monocotyledonous plant cell.

11 . The transgenic plant cell of claim 9 , wherein said transgenic plant cell is a dicotyledonous plant cell.

12 . A transgenic plant, or part thereof, comprising the recombinant DNA molecule of claim 1 .

13 . A progeny plant of the transgenic plant of claim 12 , or a part thereof, wherein the progeny plant or part thereof comprises said recombinant DNA molecule.

14 . A transgenic seed, wherein the seed comprises the recombinant DNA molecule of claim 1 .

15 . A method of producing a commodity product comprising obtaining a transgenic plant or part thereof according to claim 12 and producing the commodity product therefrom.

16 . The method of claim 15 , wherein the commodity product is protein concentrate, protein isolate, grain, starch, seeds, meal, flour, biomass, or seed oil.

17 . A method of expressing a transcribable DNA molecule comprising obtaining a transgenic plant according to claim 12 and cultivating plant, wherein the transcribable DNA is expressed.

Assignees

• Monsanto Technology Llc

Inventors

• Chittoor Jaishree M
• Flasinski Stanislaw
• Oufattole Mohammed
• Petersen Michael W

Classifications

• C07K14/415 Primary

  from plants · CPC title

• C12N15/8216 Primary

  Methods for controlling, regulating or enhancing expression of transgenes in plant cells · CPC title

• C12N15/8222

  Developmentally regulated expression systems, tissue, organ specific, temporal or spatial regulation · CPC title

• C12N15/8279 Primary

  for biotic stress resistance, pathogen resistance, disease resistance · CPC title

• C12N15/8274

  for herbicide resistance · CPC title