Modified hematopoietic stem/progenitor and non-t effector cells, and uses thereof
US-2024041933-A1 · Feb 8, 2024 · US
New method to obtain lymphoid progenitors
US2020048610A1 · US · A1
| Field | Value |
|---|---|
| Publication number | US-2020048610-A1 |
| Application number | US-201816498803-A |
| Country | US |
| Kind code | A1 |
| Filing date | Mar 26, 2018 |
| Priority date | Mar 27, 2017 |
| Publication date | Feb 13, 2020 |
| Grant date | — |
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- Title
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Abstract
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The present invention relates to a method for preparing lymphoid progenitors. The inventors took advantage of their original and relevant Warts, Hypogammaglobulinemia, Infections and Myelokathexis (WHIM) Syndrome (WS) model and the access to blood samples from five WS patients to investigate the impact of CXCR4 desensitization on BM and extra-medullary (i.e. splenic) hematopoiesis and hematopoietic stem and progenitor cells (HSPCs) recirculation. They developed, for the first time, an original in vitro system permitting to selectively expand HSPCs to obtain lymphoid progenitors by using an original cocktail of cytokines. In particular, the present invention relates to an in vitro method for preparing lymphoid progenitors by culturing HSPCs in an appropriate culture medium comprising an effective amount of a cocktail of cytokines consisting in SCF, IL-3, IL-6, IL-7, Flt-3, and CXCL12.
First claim
Opening claim text (preview).
1 . An in vitro method for preparing lymphoid progenitor cells, said method comprising the step of culturing hematopoietic stem and progenitor cells (HSPCs) in an appropriate culture medium comprising an effective amount of a cocktail of cytokines selected from the group consisting of SCF, IL-3, IL-6, IL-7 and Flt-3. 2 . The in vitro method according to claim 1 wherein the cocktail of cytokines comprises SCF, IL-3, IL-6, IL-7 and Flt-3. 3 . The in vitro method according to claim 1 , wherein the culture medium also comprises an effective amount of the chemokine CXCL12. 4 . The in vitro method according to claim 1 , wherein the culture medium further comprises a feeder. 5 . The in vitro method according to claim 1 , wherein the culture medium does not comprise a feeder. 6 . The in vitro method according to claim 1 , wherein the duration of the culturing step is in the range of 2 to 14 days.
Assignees
Inventors
Classifications
Interleukin-7 (IL-7) · CPC title
from blood or immune system cells · CPC title
Interleukin-3 (IL-3) · CPC title
Chemokines, e.g. MIP-1, MIP-2, RANTES, MCP, PF-4 · CPC title
- C12N5/0647Primary
Haematopoietic stem cells; Uncommitted or multipotent progenitors · CPC title
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- What does patent US2020048610A1 cover?
- The present invention relates to a method for preparing lymphoid progenitors. The inventors took advantage of their original and relevant Warts, Hypogammaglobulinemia, Infections and Myelokathexis (WHIM) Syndrome (WS) model and the access to blood samples from five WS patients to investigate the impact of CXCR4 desensitization on BM and extra-medullary (i.e. splenic) hematopoiesis and hematopoi…
- Who is the assignee on this patent?
- Inserm Institute National De La Sante Et De La Rech Medicale, Univ Paris Sud
- What technology area does this patent fall under?
- Primary CPC classification C12N5/0647. Mapped technology areas include Chemistry & Metallurgy.
- When was this patent published?
- Publication date Thu Feb 13 2020 00:00:00 GMT+0000 (Coordinated Universal Time) (A1). Legal status and post-grant events are not shown on this page.
- What related patents are in patentsdb?
- We list 5 related publications on this page (citations in our corpus or others sharing the same primary CPC).