A method for making a solid-supported phospholipid bilayer

Patent metadata

Field	Value
Publication number	US-2020040017-A1
Application number	US-201816486295-A
Country	US
Kind code	A1
Filing date	Feb 14, 2018
Priority date	Feb 15, 2017
Publication date	Feb 6, 2020
Grant date	—

Abstract

A method of preparing a solid-supported phospholipid bilayer is provided. The method includes a) a first step of providing a solution comprising a bicellar mixture of a long-chain phospholipid and a short-chain phospholipid; b) at least one second step of decreasing the temperature of the solution to below 0° C., increasing the temperature to above room temperature and causing the solution to be blended; and c) a third step of depositing the solution obtained after the second step on a surface of a support, wherein the concentration of the long-chain phospholipid in the solution is at most 0.1 mg/mL, for obtaining a solid-supported phospholipid bilayer. A solid-supported phospholipid layer obtained by the method as defined above is also provided.

First claim

1 . A method of preparing a solid-supported phospholipid bilayer, the method comprising:
a) a first step of providing a solution comprising a bicellar mixture of a long-chain phospholipid and a short-chain phospholipid; b) at least one second step of decreasing the temperature of the solution to below 0° C., increasing the temperature to above room temperature and causing the solution to be blended; and c) a third step of depositing the solution obtained after the second step on a surface of a support, wherein a concentration of the long-chain phospholipid in the solution is at most 0.1 mg/mL, for obtaining a solid-supported phospholipid bilayer.

2 . The method of claim 1 , wherein the long-chain phospholipid is a phospholipid comprising two fatty acid residues, wherein each of the fatty acid residues has more than 10 carbons.

3 . The method of claim 1 , wherein at least one of the long-chain phospholipid and the short-chain phospholipid is a phosphatidylcholine.

4 . The method of claim 1 , wherein the long-chain phospholipid has a gel-to-fluid-phase transition temperature of below 0° C.

5 . The method of claim 1 , wherein the short-chain phospholipid is a phospholipid comprising two fatty acid residues, wherein each of the fatty acid residues has at most 10 carbons.

6 . (canceled)

7 . (canceled)

8 . The method of claim 1 , wherein at least one of the long-chain phospholipid and the short-chain phospholipid comprises an unsaturated fatty acid residue.

9 . (canceled)

10 . (canceled)

11 . The method of claim 1 , wherein the short-chain phospholipid is 1,2-dihexyanoyl-sn-glycero-3-phosphocholine and the long-chain phospholipid is 1,2-dioleoyl-sn-glycero-3-phosphocholine.

12 . The method of claim 1 , wherein the concentration of the long-chain phospholipid in the solution of the third step is below 0.05 mg/mL.

13 . The method of claim 1 , wherein the long-chain phospholipid and the short-chain phospholipid are provided in a molar ratio of about 1:0.02 to about 1:5.

14 . The method of claim 1 , wherein the solution of the second step after blending is diluted in order to obtain the concentration of the long-chain phospholipid in the solution being at most 0.1 mg/mL.

15 . The method of claim 1 , wherein the solvent used to make the solution is a water-based buffer solution.

16 . The method of claim 1 , wherein the support is an inorganic material.

17 . The method of claim 1 , wherein the surface of the support is substantially planar.

18 . The method of claim 1 , wherein the surface of the support is nanostructured.

19 . (canceled)

20 . The method of claim 1 , wherein in the third step, the bicellar mixture ruptures in order to obtain the solid-supported phospholipid bilayer.

21 . The method of claim 1 , wherein decreasing the temperature of the solution to below 0° C. in the at least one second step comprises decreasing the temperature of the solution to below −10° C.

22 . The method of claim 1 , wherein increasing the temperature to above room temperature in the at least one second step comprises increasing the temperature to above 30° C.

23 - 25 . (canceled)

26 . The method of claim 1 , wherein the second step is repeated for about at least 2 times.

27 . The method of claim 1 , wherein the obtained solid-supported phospholipid bilayer is a planar phospholipid bilayer on the support.

28 . A solid-supported phospholipid bilayer obtained by the method of claim 1 .

29 - 32 . (canceled)

Assignees

• Univ Nanyang Tech

Inventors

• Cho Nam-Joon
• Jackman Joshua Alexander

Classifications

• B05D7/24

  for applying particular liquids or other fluent materials · CPC title

• B05D2401/10

  Organic solvent (B05D2401/21 takes precedence) · CPC title

• B05D2203/30

  Other inorganic substrates, e.g. ceramics, silicon · CPC title

• B82Y40/00

  Manufacture or treatment of nanostructures · CPC title

• C07F9/106 Primary

  Adducts, complexes, salts of phosphatides · CPC title