Genetic system for promoting recombination and gene transfer in wheat

Patent metadata

Field	Value
Publication number	US-2020037566-A1
Application number	US-201716339212-A
Country	US
Kind code	A1
Filing date	Oct 7, 2017
Priority date	Oct 7, 2016
Publication date	Feb 6, 2020
Grant date	—

Abstract

Wheat lines comprising homoeologous pairing promoter genes from chromosome 5M g from Ae. geniculata and methods of inducing homoeologous recombination in plant breeding from these lines.

First claim

1 . A wheat plant starting stock for inducing homoeologous recombination in plant breeding, wherein said starting stock comprises chromosome 5M g from Ae. geniculata and at least one chromosome bearing a pairing (Ph) gene which is not functioning.

2 . The wheat plant starting stock of claim 1 , wherein said at least one chromosome bearing a pairing (Ph) gene which is not functioning, comprises the mutant homoeologous-pairing allele ph1b.

3 . The wheat plant starting stock of claim 2 , wherein said starting stock is heterozygous for Ph1ph1b.

4 . The wheat plant starting stock of claim 1 , wherein said starting stock is heterozygous for chromosome 5M g /5D.

5 . The wheat plant starting stock of claim 1 , wherein said chromosome 5M g is a recombinant chromosome comprising one or more translocated wheat genes in distal regions of said chromosome, provided that said chromosome retains one or more native homoeologous pairing promotor gene(s) from 5M g of Ae. geniculata.

6 . The wheat plant starting stock of claim 1 , wherein said starting stock is capable of serving as a parent for breeding stable wheat cultivars.

7 . The wheat plant starting stock of claim 1 , wherein said wheat plant is a Triticum aestivum background.

8 . A method of inducing homoeologous recombination in wheat breeding, said method comprising:
providing a wheat starting stock according to claim 1 ; providing a source plant capable of crossbreeding with wheat and comprising one or more target genes for recombination with one or more wheat chromosomes; crossing said wheat starting stock with said source plant; selecting progeny from said crossing that comprises a chromosome comprising one or more of said target genes transferred in a homoeologous recombination event during said crossing.

9 . The method of claim 8 , wherein said source plant is heterozygous from said one or more target genes.

10 . The method of claim 8 , wherein said source plant is a different species from said wheat starting stock.

11 . The method of claim 8 , wherein said source plant is a wheat cultivar.

12 . The method of claim 8 , wherein said source plant comprises a functional Ph1 allele.

13 . The method of claim 8 , wherein said source plant comprise at least one chromosome bearing a pairing (Ph) gene which is not functioning.

14 . The method of claim 13 , wherein said at least one chromosome bearing a pairing (Ph) gene which is not functioning, comprises the mutant homoeologous-pairing allele ph1b.

15 . The method of claim 14 , wherein said source plant is heterozygous for Ph1/ph1b.

16 . The method of claim 8 , comprising selecting said progeny that is homozygous for ph1b/ph1b.

17 . The method of claim 16 , wherein said progeny is heterozygous for chromosome 5M g /5D.

18 . The method of claim 17 , wherein said progeny comprises a recombinant chromosome comprising one or more of said target genes.

19 . The method of claim 18 , further comprising crossing said progeny with a plant capable of crossbreeding with wheat, wherein one or more of said target genes is transferred in a second homoeologous recombination event during said crossing of said progeny.

20 . The method of claim 19 , wherein said target gene transferred during said second recombination event is located in a region of the chromosome proximal to the centromere.

21 . The method of claim 19 , wherein a plurality of genes are transferred in said second recombination event.

22 . The method of claim 19 , comprising two or more of said second recombination events.

23 . The method of claim 19 , wherein the frequency of second recombination events in said crossing is at least 3%.

24 . The method of claim 19 , wherein the frequency of second recombination events in said crossing is twenty-five times greater than recombination of said target gene when crossed with a wheat starting stock in the absence of said 5M g chromosome.

25 . The method of claim 8 , further comprising collecting the progeny seed from said crossing and growing said seeds, thus producing progeny plants expressing said one or more of said target genes.

26 . The method of claim 25 , wherein said one or more target genes confer a desirable trait to said progeny plants.

27 . A seed produced according to the method of claim 8 .

28 . A wheat grain kernel produced according to the method of claim 8 .

29 . A wheat genetic stock, Hpp-5M g /5D, ph1bph1b, for inducing homoeologous recombination in plant breeding, wherein said wheat genetic stock comprises chromosome 5M g from Ae. geniculata and two ph1b mutant alleles.

30 . The wheat genetic stock of claim 29 , further comprising one or more target genes for recombination with one or more wheat chromosomes.

Assignees

• Univ Kansas State

Inventors

• Gill Bikram S
• Friebe Bernd
• Koo Dal-Hoe

Classifications

• A01H5/10 Primary

  Seeds · CPC title

• A01H6/4678 Primary

  Triticum sp. [wheat] · CPC title