Processing biomass
US-2015353974-A1 · Dec 10, 2015 · US
Microorganism producing o-acetyl homoserine and the method of producing o-acetyl homoserine using the same
US2020024626A1 · US · A1
| Field | Value |
|---|---|
| Publication number | US-2020024626-A1 |
| Application number | US-201916577837-A |
| Country | US |
| Kind code | A1 |
| Filing date | Sep 20, 2019 |
| Priority date | Jun 23, 2014 |
| Publication date | Jan 23, 2020 |
| Grant date | — |
How to read this patent
A practical reading order for non-experts. Skip the full description unless you need deep technical detail.
- Title
What the patent document calls the invention.
- Abstract
A short plain-language summary of the technical disclosure.
- Assignees and inventors
Who owns or filed the patent and who is credited as inventor.
- Key dates
Filing, priority, publication, and grant dates set the timeline.
- First independent claim
The legal scope of protection — read this for what is actually claimed.
- CPC / IPC classifications
Technology tags used to group this patent with similar filings.
- Citations and related patents
Prior art links and similar publications in this corpus.
Abstract
Official abstract text for this publication.
Disclosed is a microorganism of Escherichia sp. producing O-acetyl homoserine, and a method of producing O-acetyl homoserine in high yield using the microorganism.
First claim
Opening claim text (preview).
1 . A method for producing L-methionine, comprising: (a) culturing a microorganism of Escherichia sp. producing O-acetyl homoserine, wherein in the microorganism, the endogenous activity of citrate synthase is attenuated or inactivated, and a homoserine O-acetyltransferase is further introduced or enhanced, or an endogenous homoserine O-succinyltransferase is further modified to a polypeptide having 95% identity or above with SEQ ID NO: 16 and further having mutations at the G111E position and L112T or L112H positions to have the activity of homoserine O-acetyltransferase; and (b) contacting O-acetyl homoserine produced in step (a) with O-acetyl homoserine sulfhydrylase, or a microorganism having O-acetyl homoserine sulfhydrylase. 2 . The method according to claim 1 , wherein the microorganism with the attenuated endogenous activity of citrate synthase has an amino acid sequence of SEQ ID NO: 1 or SEQ ID NO: 2. 3 . The method according to claim 1 , wherein in the microorganism, the activity of cystathionine gamma synthase, homoserine kinase, or both is further attenuated or inactivated compared to their endogenous activities. 4 . The method according to claim 1 , wherein in the microorganism, the activity of at least one protein selected from the group consisting of phosphoenolpyruvate carboxylase, aspartate aminotransferase, and aspartate semialdehyde dehydrogenase is further introduced or enhanced. 5 . The method according to claim 1 , wherein the microorganism is Escherichia coli. 6 . The method according to claim 1 , wherein O-acetyl homoserine sulfhydrylase is derived from Leptospira sp., Chromobacterium sp., or Hyphomonas sp. 7 . The method according to claim 1 , further comprising adding methylmercaptan as substrate in step (b). 8 . The method according to claim 1 , further comprising recovering O-acetyl homoserine produced in step (a). 9 . The method according to claim 1 , further comprising recovering L-methionine produced in step (b).
Assignees
Inventors
Classifications
Phosphoenolpyruvate carboxylase (4.1.1.31) · CPC title
Phosphotransferases with an alcohol group as acceptor (2.7.1), e.g. protein kinases · CPC title
Lyases (4.) · CPC title
- C12P13/06Primary
Alanine; Leucine; Isoleucine; Serine; Homoserine · CPC title
Citrate (Si)-synthase (2.3.3.1) · CPC title
Patent family
Related publications grouped by family.
External sources
Frequently asked questions
Answers are generated from the same data shown on this page.
- What does patent US2020024626A1 cover?
- Disclosed is a microorganism of Escherichia sp. producing O-acetyl homoserine, and a method of producing O-acetyl homoserine in high yield using the microorganism.
- Who is the assignee on this patent?
- Cj Cheiljedang Corp
- What technology area does this patent fall under?
- Primary CPC classification C12P13/06. Mapped technology areas include Chemistry & Metallurgy.
- When was this patent published?
- Publication date Thu Jan 23 2020 00:00:00 GMT+0000 (Coordinated Universal Time) (A1). Legal status and post-grant events are not shown on this page.
- What related patents are in patentsdb?
- We list 8 related publications on this page (citations in our corpus or others sharing the same primary CPC).