Abcb5(+) stem cells for treating ocular disease
US-2015374756-A1 · Dec 31, 2015 · US
Method for controlling differentiation of pluripotent stem cells
US2020010800A1 · US · A1
| Field | Value |
|---|---|
| Publication number | US-2020010800-A1 |
| Application number | US-201816482160-A |
| Country | US |
| Kind code | A1 |
| Filing date | Jan 31, 2018 |
| Priority date | Jan 31, 2017 |
| Publication date | Jan 9, 2020 |
| Grant date | — |
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Abstract
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The present invention relates to a method for controlling differentiation of pluripotent stem cells, which method comprises selecting a laminin or a fragment thereof based on binding affinity for the pluripotent stem cells and inducing differentiation of the pluripotent stem cells in the presence of the laminin or a fragment thereof. Here, the binding affinity for cells can be assessed by time-course observation of the survival rate and motility of the cells. According to the present invention, a cell population containing any desired proportion of differentiated cells can be produced from pluripotent stem cells in a simple manner. The cell population obtained by this production method is very useful for cell therapy-based treatment strategies for diseases.
First claim
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1 . A method for controlling differentiation of pluripotent stem cells toward corneal epithelial cells, the method comprising: inducing differentiation of the pluripotent stem cells in the presence of laminin 332 or a laminin 332E8 fragment, which directs differentiation toward corneal epithelial cells. 2 - 12 . (canceled) 13 . A method for controlling differentiation of pluripotent stem cells toward neural crest cells, the method comprising inducing differentiation of the pluripotent stem cells in the presence of laminin 211 or a laminin 211E8 fragment, which directs differentiation toward neural crest cells. 14 . A method for controlling differentiation of pluripotent stem cells toward retinal cells or neural crest cells, the method comprising inducing differentiation of the pluripotent stem cells in the presence of laminin 411 or a laminin 411E8 fragment, which directs differentiation toward retinal cells or neural crest cells. 15 . A method for producing an eye-related cell population, comprising culturing differentiated cells derived from pluripotent stem cells by the method according to claim 1 . 16 . The method according to claim 15 , wherein the cell population to be produced is a corneal epithelial cell population. 17 . A method for fabricating a corneal epithelial cell sheet for transplantation using a corneal epithelial cell population produced by the method according to claim 16 . 18 . A method for producing an eye-related cell population, comprising culturing differentiated cells derived from pluripotent stem cells by the method according to claim 13 . 19 . A method for producing an eye-related cell population, comprising culturing differentiated cells derived from pluripotent stem cells by the method according to claim 14 .
Assignees
Inventors
Classifications
Proteins not provided for elsewhere · CPC title
- C12N5/0621Primary
Eye cells, e.g. cornea, iris pigmented cells (photoreceptors C12N5/062) · CPC title
Fibronectin; Laminin · CPC title
from artificially induced pluripotent stem cells · CPC title
Growth factors; Growth regulators · CPC title
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- What does patent US2020010800A1 cover?
- The present invention relates to a method for controlling differentiation of pluripotent stem cells, which method comprises selecting a laminin or a fragment thereof based on binding affinity for the pluripotent stem cells and inducing differentiation of the pluripotent stem cells in the presence of the laminin or a fragment thereof. Here, the binding affinity for cells can be assessed by time-…
- Who is the assignee on this patent?
- Univ Osaka
- What technology area does this patent fall under?
- Primary CPC classification C12N5/0621. Mapped technology areas include Chemistry & Metallurgy.
- When was this patent published?
- Publication date Thu Jan 09 2020 00:00:00 GMT+0000 (Coordinated Universal Time) (A1). Legal status and post-grant events are not shown on this page.
- What related patents are in patentsdb?
- We list 8 related publications on this page (citations in our corpus or others sharing the same primary CPC).