Biological method for preparing heme iron not derived from porcine blood

Patent metadata

Field	Value
Publication number	US-2019352680-A1
Application number	US-201716475409-A
Country	US
Kind code	A1
Filing date	Dec 6, 2017
Priority date	Jan 3, 2017
Publication date	Nov 21, 2019
Grant date	—

Abstract

The present invention relates to heme iron not derived from porcine blood and a method of preparing the same, and more particularly to a method of biologically preparing heme iron not derived from porcine blood, a method of preparing a salt thereof, and an iron supplement containing the salt thus prepared as an active ingredient.

First claim

1 . A method of biologically preparing a salt of heme iron of Chemical Formula 1 below, not derived from porcine blood and containing no animal component, the method comprising:
A) producing heme iron by culturing Escherichia coli DH5α pLEX_HMD (Accession number: KCTC 13173BP) at 39 to 44° C. and a pH of 7 to 9 in a medium containing no animal component; B) purifying the heme iron produced in step A); and C) preparing a salt of the heme iron purified in step B).

2 . The method of claim 1 , wherein the medium includes plant-derived peptone and a yeast extract prepared from yeast cultured in a plant-derived medium.

3 . The method of claim 1 , wherein the pH is adjusted using succinic acid.

4 . The method of claim 1 , wherein step B) is performed through filtration process using diatomaceous earth.

5 . The method of claim 4 , wherein the diatomaceous earth is Celite®545.

6 . A salt of heme iron of Chemical Formula 1 below not derived from porcine blood and containing no animal component, prepared by the method of claim 1 .

7 . A pharmaceutical composition for preventing or treating iron-deficiency anemia, containing the salt of heme iron of claim 6 as an active ingredient, without containing an animal component.

Assignees

• Intron Biotechnology Inc

Inventors

• Yoon Seong Jun
• Pyo Jung In
• Hwang Soon Hye
• Jun Soo Youn
• Kang Sang Hyeon

Classifications

• C07D487/22

  in which the condensed system contains four or more hetero rings · CPC title

• C12P9/00 Primary

  Preparation of organic compounds containing a metal or atom other than H, N, C, O, S or halogen {(phosphoglycerides, C12P7/6481)} · CPC title

• A61K33/26

  Iron; Compounds thereof · CPC title

• C12N1/20

  Bacteria; Culture media therefor · CPC title

• C07F15/02

  Iron compounds · CPC title