Nerve cell production method

Patent metadata

Field	Value
Publication number	US-2019352604-A1
Application number	US-201816485405-A
Country	US
Kind code	A1
Filing date	Feb 23, 2018
Priority date	Feb 24, 2017
Publication date	Nov 21, 2019
Grant date	—

Abstract

The present invention provides a nerve cell production method that involves preparing stem cells and then introducing inducing-factor RNA into the stem cells and allowing differentiation into nerve cells. The present invention also provides a nerve cell production method that involves preparing cells, introducing reprogramming-factor RNA into the cells, introducing inducing-factor RNA into the cells into which the reprogramming-factor RNA was introduced, and allowing differentiation into nerve cells.

First claim

1 . A nerve cell production method comprising:
preparing stem cells, and introducing inducing factor RNA into the stem cells and causing their differentiation into inhibitory neurons, wherein the inducing factor RNA includes at least one of ASCL mRNA and DLX mRNA.

2 . (canceled)

3 . The nerve cell production method according to claim 1 , wherein the inducing factor RNA further includes MYT mRNA.

4 . The nerve cell production method according to claim 1 , wherein the inhibitory neurons express GAD.

5 .- 9 . (canceled)

10 . A nerve cell production method comprising:
preparing cells, introducing reprogramming factor RNA into the cells, and introducing inducing factor RNA into the cells in which the reprogramming factor RNA has been introduced, to cause their differentiation into nerve cells.

11 .- 21 . (canceled)

22 . RNA corresponding to DNA of any one of SEQ ID NO: 1 to 10.

23 . An inducing factor comprising RNA corresponding to DNA of any one of SEQ ID NO: 1 to 10.

24 . A nerve cell production method comprising:
preparing stem cells, and introducing inducing factor RNA into the stem cells and causing their differentiation into dopamine-producing neurons, wherein the inducing factor RNA includes at least one selected from the group consisting of NGN mRNA, ASCL mRNA, NURR mRNA, LMX mRNA, EN mRNA, PITX mRNA, and FOXA mRNA.

25 . The nerve cell production method according to claim 24 , wherein the dopamine-producing neurons express TH.

26 . A nerve cell production method comprising:
preparing stem cells, and introducing inducing factor RNA into the stem cells and causing their differentiation into excitatory neurons, wherein the inducing factor RNA includes NGN mRNA.

27 . The nerve cell production method according to claim 26 , wherein the excitatory neurons express vGLUT.

28 . A nerve cell production method comprising:
preparing stem cells, and introducing inducing factor RNA into the stem cells and causing their differentiation into nerve cells, wherein the inducing factor RNA includes mRNA corresponding to a drug resistance gene, and the drug is at least one selected from among blasticidin, puromycin, hygromycin, neomycin, G418, and zeocin.

29 . A nerve cell production method comprising:
preparing cells, introducing reprogramming factor RNA into the cells, and introducing inducing factor RNA into the cells in which the reprogramming factor RNA has been introduced, to cause their differentiation into nerve cells, wherein the inducing factor RNA includes mRNA corresponding to a drug resistance gene, and the drug is at least one selected from among blasticidin, puromycin, hygromycin, neomycin, G418, and zeocin.

Assignees

• Tanabe Koji
• I Peace Inc

Inventors

• Tanabe Koji
• Kelly Brendan

Classifications

• C12N2506/45

  from artificially induced pluripotent stem cells · CPC title

• C12N2501/65

  MicroRNA · CPC title

• C12N2501/15

  Transforming growth factor beta (TGF-β) · CPC title

• C12N2510/00

  Genetically modified cells · CPC title

• C12N15/113

  Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; {Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing (when used in plants C12N15/8218)} · CPC title