Flow cell with flexible connection

Patent metadata

Field	Value
Publication number	US-2019351413-A1
Application number	US-201916401805-A
Country	US
Kind code	A1
Filing date	May 2, 2019
Priority date	May 15, 2018
Publication date	Nov 21, 2019
Grant date	—

Abstract

An instrument includes a reagent management system. The reagent management system includes a plurality of reagent wells, each reagent well operable to contain a reagent of a plurality of reagents positioned therein. The reagent management system is operable to select a flow of reagent from one of the plurality of reagents. A flexible connection includes a laminate stack and includes a first flexible channel in fluid communication with the reagent management system. The first flexible channel is operable to route the flow of reagent therethrough. A flow cell includes a flow channel in fluid communication with the first flexible channel. The flow channel is operable to route the flow of reagent over analytes positioned in the flow channel. The flexible connection enables the flow cell to be moved by the instrument relative to a fixed reference point in the instrument.

First claim

What is claimed is:

1 . An instrument comprising:
a reagent management system, operable to be positioned in the instrument, the reagent management system comprising a plurality of reagent wells, each reagent well operable to contain a reagent of a plurality of reagents positioned therein, the reagent management system operable to select a flow of reagent from one of the plurality of reagent wells; a flexible connection comprised of a laminate stack and operable to be positioned in the instrument, the flexible connection comprising a first flexible channel in fluid communication with the reagent management system, the first flexible channel operable to route the flow of reagent therethrough; and a flow cell, operable to be positioned in the instrument, the flow cell comprising a flow channel in fluid communication with the first flexible channel, the flow channel operable to route the flow of reagent over analytes positioned in the flow channel; and a detection module; wherein the flow cell is moveable by the instrument relative to a fixed reference point in the instrument.

2 . The instrument of claim 1 , wherein the flow cell is moveable relative to the fixed reference point in the instrument while the detection module is held stationary relative to the reference point.

3 . The instrument of claim 1 , comprising:
a cartridge, the cartridge comprising the reagent management system, the flow cell and the flexible connection; wherein, when the cartridge is engaged with the instrument and the flow cell is engaged with the cartridge, the reagent management system is fixed relative to the reference point of the instrument while the flow cell is movable relative to the reference point of the instrument.

4 . The instrument of claim 1 , wherein:
the reagent management system is positioned relative to the reference point within about a predetermined first tolerance range; and the flow cell is positioned relative to the reference point within about a second predetermined tolerance range, the first tolerance range being at least 10 times greater than the second tolerance range.

5 . The instrument of claim 1 , wherein the flexible connection comprises a second flexible channel in fluid communication with the flow channel of the flow cell, the second flexible channel operable to route the flow of reagent from the flow cell to the reagent management system after the flow of reagent has passed through the flow channel.

6 . The instrument of claim 5 , wherein the flexible connection comprises a slit positioned between the first flexible channel and the second flexible channel.

7 . The instrument of claim 1 , wherein the flexible connection comprises a sinuous shape.

8 . The instrument of claim 1 , wherein the flexible connection comprises:
a top layer defining a top of the first flexible channel; a bottom layer defining a bottom of the first flexible channel; and an intermediate layer defining a wall width and a channel width of the first flexible channel; wherein a ratio of the wall width to the channel width is greater than about 2.5.

9 . The instrument of claim 8 , wherein the intermediate layer is a plurality of sublayers.

10 . The instrument of claim 8 , wherein the top layer, intermediate layer and bottom layer are bonded together utilizing one of an adhesive bonding process, a thermal bonding process, or a direct laser bonding process.

11 . The instrument of claim 1 , wherein, as the flow of reagent is routed through the flow channel, a chemical reaction is performed between the flow of reagent and the analytes, the chemical reaction inducing the analytes to affect detectable properties related to the analytes; and
wherein the detection module is operable to detect the detectable properties.

12 . The instrument of claim 1 , comprising a mechanical strain relief element fixedly coupled to the flexible connection.

13 . The instrument of claim 12 , wherein the mechanical strain relief element is one of:
an epoxy bead, a trough, or a solid piece having a first adhesive and a second adhesive bonded thereon.

14 . A cartridge comprising:
a reagent management system operable to select a flow of reagent from one of a plurality of reagents contained in the reagent management system; a flexible connection formed from a laminate stack and comprising a first flexible channel in fluid communication with the reagent management system, the first flexible channel being operable to route the flow of reagent therethrough; and a flow cell comprising a flow channel in fluid communication with the first flexible channel, the flow channel operable to route the flow of reagents over analytes positioned in the flow channel; wherein the flexible connection enables the flow cell to be moved relative to the reagent management system.

15 . The cartridge of claim 14 , wherein the flexible connection comprises a second flexible channel in fluid communication with the flow channel of the flow cell, the second flexible channel operable to route the flow of reagent from the flow cell to the reagent management system after the flow of reagent has passed through the flow channel.

16 . The cartridge of claim 15 , wherein the flexible connection comprises a slit positioned between the first flexible channel and the second flexible channel.

17 . The cartridge of claim 14 , wherein the flexible connection comprises a sinuous shape.

18 . The cartridge of claim 14 , wherein the flexible connection comprises:
a top layer defining a top of the first flexible channel; a bottom layer defining a bottom of the first flexible channel; and an intermediate layer defining a wall width and a channel width of the first flexible channel; wherein a ratio of the wall width to the channel width is greater than about 2.5.

19 . The cartridge of claim 14 , comprising a mechanical strain relief element fixedly coupled to the flexible connection.

20 . The cartridge of claim 19 , wherein the mechanical strain relief element is one of:
an epoxy bead, a trough, or a solid piece having a first adhesive and a second adhesive bonded thereon.

21 . A flexible connection module comprising:
a flexible connection formed from a laminate stack and comprising a first channel inlet via, a first channel outlet via, and a first flexible channel in fluid communication therebetween, wherein the first channel inlet via comprises a fluidic seal operable to connect to are agent management system outlet port and to enable a flow of reagent therethrough; and a flow cell comprising an inlet port, an outlet port, and a flow channel in fluid communication therebetween, wherein the inlet port is in fluid communication with the first channel outlet via of the flexible connection, the flow channel operable to route the flow of reagent over analytes positioned in the flow channel.

22 . The flexible connection module of claim 21 , wherein the flexible connection comprises:
a second channel inlet via, a second channel outlet via and a second flexible channel in fluid communication therebetween; wherein the second channel inlet via is in fluid communication with the outlet port of the flow cell; and wherein the second channel outlet via comprises a fluidic seal operable to connect to a reagent management system inlet port and to enable the flow of reagent therethrough.

23 . The module of claim 21 , wherein the fluidic seal is a detachable fluidic seal operable to detach

Assignees

• Illumina Inc

Inventors

• Delattre Cyril
• Rhee Minsoung
• Liu Jeffrey
• Cox-Muranami Wesley
• Crivelli Paul
• Foley Jennifer
• Segale Darren
• Taylor Jay
• Hage Matthew
• Paik Philip
• Allegoren Erik
• Hertzog David
• Moroz-Smietana Alex
• Ma Xiaoxiao
• Takahashi Tsukasa
• Westerberg Brandon

Classifications

• G01N2035/0439

  Rotary sample carriers, i.e. carousels · CPC title

• G01N2035/0401

  Sample carriers, cuvettes or reaction vessels · CPC title

• G01N35/04

  Details of the conveyor system {(G01N35/021 - G01N35/028 take precedence)} · CPC title

• G01N35/085

  Flow Injection Analysis · CPC title

• B01L2200/04

  Exchange or ejection of cartridges, containers or reservoirs · CPC title