Method for extraction of an agent from a plant source

Patent metadata

Field	Value
Publication number	US-2019314432-A1
Application number	US-201716338280-A
Country	US
Kind code	A1
Filing date	Sep 28, 2017
Priority date	Sep 29, 2016
Publication date	Oct 17, 2019
Grant date	—

Abstract

Provided are methods for extraction of various agents, e.g. active agent, from a plant source, specifically with the use of extraction formulations which are based on micellar liquid systems.

First claim

1 . A process for extraction of a lipophilic agent from a plant source, the process comprising:
(a) obtaining a first mixture comprising a first quantity of a plant source containing the lipophilic agent and a first quantity of an extraction medium, the extraction medium comprising at least one oil, at least one hydrophilic surfactant, at least co-surfactant and optionally at least one co-solvent; (b) homogenizing the first mixture; and (c) separating the homogenized mixture into a biomass slurry and an agent-loaded medium.

2 . The process of claim 1 , wherein the agent-loaded medium comprises between about 0.02 and 20 wt % of the lipophilic agent.

3 . The process of claim 1 , wherein the homogenization of step (b) is carried out in at least one condition selected from (i) a period of time of between about 1 minute and about 120 minutes, (ii) at a pressure of between about 500 and 6,000 psi, and (iii) at a temperature of between about 5 and about 70° C.

4 .- 9 . (canceled)

10 . The process of claim 1 , wherein the weight ratio (wt/wt) of plant source to said first quantity of system is between 1:5 and 1:80.

11 . The process of claim 1 , further comprising:
(d) mixing the biomass slurry with a second quantity of the extraction medium to obtain a second mixture; (e) homogenizing the second mixture; and (f) separating the second mixture into biomass slurry and agent-loaded medium.

12 . (canceled)

13 . The process of claim 1 , further comprising:
(d′) mixing the agent-loaded medium with a second quantity of the plant source to obtain a second mixture; (e′) homogenizing the second mixture; and (f′) separating the second mixture into biomass slurry and highly agent-loaded medium.

14 . (canceled)

15 . The process of claim 1 , wherein said at least one oil is selected from essential oils, D-limonene, mineral oil, paraffinic oils, phospholipids, polar lipids, squalenes, spingomelines, waxes, vegetable oils, glycerides, triglycerides, fatty acids and esters of fatty acids, and liquid hydrocarbons, wherein said at least one oil being present in the microemulsion at an amount of between about 0.5 and 20 wt %.

16 . The process of claim 1 , wherein said at least one hydrophilic surfactant is selected from Solutol HS15, polyoxyethylene sorbitan monolaurate, polyoxyethylene sorbitan monopalmitate, polyoxyethylene sorbitan monooleate, and polyoxyeyhylene esters of saturated and unsaturated castor oil, ethoxylated monglycerol esters, ethoxylated fatty acids, ethoxylated fatty acids of short, medium and long chain fatty acids, wherein said at least one hydrophilic surfactant being present in the microemulsion at an amount of between about 30 and 85 wt %.

17 . The process of claim 1 , wherein said at least one co-surfactant is selected from polypropylene glycol, polyethylene glycol, sorbitol, xylitol, PEG 200, PEG 400 and PEG 600, wherein said at least one co-surfactant being present in the microemulsion at an amount of between about 1 and 50 wt %.

18 . The process of claim 1 , wherein the extraction medium further comprises at least one phospholipid, in an amount of between about 1 and 10 wt % of phospholipids; and/or at least one solvent, wherein the extraction medium comprises between about 0.1 and 25 wt % of said solvent.

19 .- 20 . (canceled)

21 . The process of claim 1 , wherein the extraction medium is essentially free of water.

22 .- 25 . (canceled)

26 . The process of claim 1 , wherein the lipophilic agent to be extracted is selected from astaxanthin, lycopene, beta-carotene, lutein, eugenol, piperin, anthocyanins, betain, oleuropein, trimyristin, curcumin, capsaicin, gossipol, rosmanol, chlorogenig acid, cynamaldehyde, flavones, caffeine, isoflavone, tocopherol, omega fatty acids (including DHA and EPA), chlorogenic acid, caffeic acid, niacin, nicotinamide, falvanoids, cineole, borneol, thujone, carnosol, carnosic acid, fumaric acid, behenic acid; and any of their triglycerides, or esters of long chain fatty acids.

27 . The process of claim 1 , wherein the plant source is an algae or a microalgae.

28 . The process of claim 27 , wherein the microalgae is Haematococcus pluvialis and the lipophilic agent is astaxanthin.

29 . (canceled)

30 . An extraction medium for extraction of a lipophilic agent from a plant source containing said lipophilic agent, comprising at least one oil, at least one hydrophilic surfactant, and at least one co-surfactant, the microemulsion optionally further comprising at least one phospholipid and/or at least one solvent.

31 . The extraction medium of claim 30 , being substantially devoid of water.

32 . (canceled)

33 . The extraction medium of claim 30 , wherein said at least one oil is selected from essential oils, terpenes, D-limonene, mineral oil, paraffinic oils, phospholipids, polar lipids, squalenes, spingomelines, waxes, vegetable oils, glycerides, triglycerides, fatty acids and esters of fatty acids, and liquid hydrocarbons, wherein said at least one oil being present in the extraction medium at an amount of between about 0.5 and 20 wt %.

34 . The extraction medium of claim 30 , wherein said at least one hydrophilic surfactant is selected from Solutol HS15, polyoxyethylene sorbitan monolaurate, polyoxyethylene sorbitan monopalmitate, polyoxyethylene sorbitan monooleate, and polyoxyeyhylene esters of saturated and unsaturated castor oil, ethoxylated monglycerol esters, ethoxylated fatty acids, ethoxylated fatty acids of short, medium and long chain fatty acids, wherein said at least one hydrophilic surfactant being present in the extraction medium at an amount of between about 30 and 85 wt %.

35 . The extraction medium of claim 30 , wherein said at least one co-surfactant is selected from polypropylene glycol, polyethylene glycol, sorbitol, xylitol, PEG 200, PEG 400 and PEG 600, wherein said at least one co-surfactant being present in the extraction medium at an amount of between about 1 and 50 wt %.

36 . The extraction medium of claim 30 , wherein the extraction medium further comprises at least one phospholipid, in an amount of between about 1 and 10 wt % of phospholipids; and/or at least one solvent in an amount of between about 0.1 and 25 wt %.

37 .- 39 . (canceled)

40 . A lipophilic agent loaded medium comprising the extraction medium of claim 30 and at least 0.1 wt % of the lipophilic agent loaded into the extraction medium.

41 . A composition comprising the lipophilic agent loaded-medium of claim 40 , the composition being selected from a food composition, a food supplement composition, a nutraceutical composition, a cosmetic composition and a pharmaceutical composition.

42 .- 47 . (canceled)

Assignees

• Yissum Res Dev Co Of Hebrew Univ Jerusalem Ltd

Inventors

• Garti Nissim
• Garti Levi Sharon
• Edri Rotem

Classifications

• A61K2236/35

  Extraction with lipophilic solvents, e.g. Hexane or petrol ether · CPC title

• A61K9/0053

  Mouth and digestive tract, i.e. intraoral and peroral administration · CPC title

• A61K9/0014

  Skin, i.e. galenical aspects of topical compositions (non-active ingredients are additionally classified in A61K47/00; A61K9/0009, A61K9/0021, A61K9/7015, A61K9/7023 take precedence; cosmetic preparations A61K8/00, A61Q; preparations for wound dressings or bandages A61L26/00) · CPC title

• A61K47/46

  Ingredients of undetermined constitution or reaction products thereof, e.g. skin, bone, milk, cotton fibre, eggshell, oxgall or plant extracts · CPC title

• A61K36/05 Primary

  Chlorophycota or chlorophyta (green algae), e.g. Chlorella · CPC title