Cleaning agent containing amylases

Patent metadata

Field	Value
Publication number	US-2019284510-A1
Application number	US-201916425317-A
Country	US
Kind code	A1
Filing date	May 29, 2019
Priority date	Jun 30, 2014
Publication date	Sep 19, 2019
Grant date	—

Abstract

A cleaning agent for hard surfaces and methods for cleaning are provided herein. In one embodiment, the cleaning agent includes at least one first amylase, wherein the first amylase is an α-amylase from Bacillus sp. No. 707 or a functional fragment or a variant thereof. The cleaning agent further includes at least one second amylase, wherein the second amylase is an AA560 α-amylase from Bacillus sp. or a functional fragment or a variant thereof. In another embodiment, the method includes dispensing the cleaning agent into the interior of an automatic dishwasher while a dishwashing program is being executed, before the main washing cycle begins, or in the course of the main washing cycle.

First claim

What is claimed is:

1 - 15 . (canceled)

16 . A cleaning agent for hard surfaces, said agent comprising
at least a first and a second amylase, (a) the first amylase being an α-amylase from Bacillus sp. no. 707 or a functional fragment or a functional variant thereof, (b) the second amylase being an AA560 α-amylase from Bacillus sp. or a functional fragment or a functional variant thereof, and at least one protease comprising (a) a subtilisin 309 from Bacillus lentus or a functional fragment or a functional variant thereof, which has an amino acid sequence which is at least 80% identical to the amino acid sequence specified in SEQ ID NO:3 over the entire length thereof and has at least one amino acid substitution at one of the positions 9, 15, 66, 212 and 239 in the numbering according to SEQ ID NO:3; and/or (b) a subtilisin 309 from Bacillus lentus or a functional fragment or a functional variant thereof which comprises an amino acid sequence which is at least 80% identical to the amino acid sequence specified in SEQ ID NO:3 over the entire length thereof and has an amino acid substitution at the position 99 and an insertion of an amino acid between the amino acids at positions 99 and 100 in the numbering according to SEQ ID NO:3; and/or (c) an alkaline protease from Bacillus lentus DSM 5483 or a functional fragment or a functional variant thereof which is at least 80% identical to the amino acid sequence specified in SEQ ID NO:4 over the entire length thereof and optionally has at least one amino acid substitution at one, two, three or four of the following positions 3, 4, 99 and 199 in the numbering according to SEQ ID NO:4, and optionally has at least one of the amino acid substitutions S3T, V4I and V199I; and/or (d) having an amino acid sequence according to one of SEQ ID NOs: 5-8.

17 . The cleaning agent according to claim 16 wherein the first amylase comprises an amino acid sequence which is at least 80% identical to the amino acid sequence specified in SEQ ID NO:1 over the entire length thereof and optionally has at least one amino acid substitution at one of the positions 172, 202, 208, 255 and 261 in the numbering according to SEQ ID NO:1.

18 . The cleaning agent according to claim 16 wherein the second amylase comprises an amino acid sequence which is at least 80% identical to the amino acid sequence specified in SEQ ID NO:2 over the entire length thereof.

19 . The cleaning agent according to claim 18 wherein the second amylase in the numbering according to SEQ ID NO:2 has an amino acid sequence at three or more of the positions 9, 26, 149, 182, 186, 202, 257, 295, 299, 323, 339 and 345 and optionally one or more, of the substitutions and/or deletions at the positions: 118, 183, 184, 195, 320 and 458.

20 . The cleaning agent according to claim 18 wherein the second amylase in the numbering according to SEQ ID NO:2 has the following amino acid substitutions and/or deletions:
(i) M9L+M323T;
(ii) M9L+M202L/T/V/I+M323T;
(iii) M9L+N195F+M202L/T/V/I+M323T;
(iv) M9L+R118K+D183*+G184*+R320K+M323T+R458K;
(v) M9L+R118K+D183*+G184*+M202L/T/V/I+R320K+M323T+R458K;
(vi) M9L+G149A+G182T+G186A+M202L+T257I+Y295F+N299Y+M323T+A339S+E345R;
(vii) M9L+G149A+G182T+G186A+M202I+T257I+Y295F+N299Y+M323T+A339S+E345R;
(viii) M9L+R118K+G149A+G182T+D183*+G184*+G186A+M202L+T257I+Y295F+N299Y+R320K+M323T+A339S+E345R+R458K;
(ix) M9L+R118K+G149A+G182T+D183*+G184*+G186A +N195F+M202L+T257I+Y295F+N299Y+R320K+M323T+A339S+E345R+R458K;
(x) M9L+R118K+G149A+G182T+D183*+G184*+G186A+M202I+T257I+Y295F+N299Y+R320K+M323T+A339S+E345R+R458K;
(xi) M9L+R118K+D183*+D184*+N195F+M202L+R320K+M323T+R458K;
(xii) M9L+R118K+D183*+D184*+N195F+M202T+R320K+M323T+R458K;
(xiii) M9L+R118K+D183*+D184*+N195F+M202I+R320K+M323T+R458K;
(xiv) M9L+R118K+D183*+D184*+N195F+M202V+R320K+M323T+R458K;
(xv) M9L+R118K+N150H+D183*+D184*+N195F+M202L+V214T+R320K+M323T+R458K; or
(xvi) M9L+R118K+D183*+D184*+N195F+M202L+V214T+R320K+M323T+E345N+R458K.

21 . The cleaning agent according to claim 16 wherein the at least one protease comprises a combination of the protease (a) with protease (b) and/or protease (c).

22 . The cleaning agent according to claim 16 wherein the first amylase and the second amylase are present in a mass ratio of from 50:1 to 1:50 based on an amount of active protein in the first amylase and the second amylase, respectively.

23 . The cleaning agent according to claim 16 wherein the weight proportion of each of the amylases and optionally of each of the proteases, based on a corresponding active protein, with respect to a total weight of the cleaning agent is of from 1×10 −8 to 5 wt. %.

24 . The cleaning agent according claim 16 comprising at least one further component, chosen from builders, surfactants, polymers, bleaching agents, bleach catalysts, bleach activators, non-protease and non-amylase enzymes, corrosion inhibitors, glass corrosion inhibitors, disintegration auxiliaries, fragrances and perfume carriers.

25 . The cleaning agent according to claim 16 wherein the at least one protease comprises a subtilisin 309 from Bacillus lentus or a functional fragment or a functional variant thereof which has an amino acid sequence which is 100% identical to the amino acid sequence specified in SEQ ID NO:3 over the entire length thereof and has at least one amino acid substitution at one of the positions chosen from S9R, A15T, V66A, N212D and Q239R.

26 . The cleaning agent according to claim 16 wherein the at least one protease comprises a subtilisin 309 from Bacillus lentus or a functional fragment or a functional variant thereof which comprises an amino acid sequence which is 100% identical to the amino acid sequence specified in SEQ ID NO:3 over the entire length thereof and has an amino acid substitution at the position 99 and an insertion of an amino acid between the amino acids at positions 99 and 100 in the numbering according to SEQ ID NO:3, wherein the positions are chosen from S99A and S99_G100InsD.

27 . The cleaning agent according to claim 16 wherein the at least one protease comprises an alkaline protease from Bacillus lentus DSM 5483 or a functional fragment or a functional variant thereof which has an amino acid sequence which is 100% identical to the amino acid sequence specified in SEQ ID NO:4 over the entire length thereof and has at least one amino acid substitution at positions R99E, R99D, S3T, V4I and V199I according to SEQ ID NO:4.

28 . The cleaning agent according to claim 16 wherein the first amylase comprises an amino acid sequence which is at least 80% identical to the amino acid sequence specified in SEQ ID NO:1 over the entire length thereof and has at least one amino acid substitution at one of the positions M202L, M202V, M202S, M202T, M202I, M202Q, M202W, S255N and R172Q.

29 . The cleaning agent according to claim 16 wherein the second amylase comprises an amino acid sequence which is at least 80% identical to the amino acid sequence specified in SEQ ID NO:2 over the entire length thereof and has at least one amino acid substitution at one of the positions 9, 26, 30, 33, 82, 37, 106, 118, 128, 133, 149, 150, 160, 178, 182, 186, 193, 195, 202, 203, 214, 231, 256, 257, 258, 269, 270, 272, 283, 295, 296, 298, 299, 303, 304, 305, 311, 314, 315, 318, 319, 320, 323, 339, 345, 361, 378, 383, 419, 421, 437, 441, 444, 445, 446, 447, 450, 458, 461, 471, 482 and 484 and/or one of the deletions D183* and G184* in the numbering according to SEQ ID NO:2.

30 . The cleaning agent according to claim 29 wherein the second amylase in the numbering according to SEQ I

Assignees

• Henkel Ag & Co Kgaa

Inventors

• Vockenroth Inga Kerstin
• Weber Thomas
• Menke Silke
• Eiting Thomas
• Mussmann Nina
• Bastigkeit Thorsten
• Wrubbel Noelle

Classifications

• C12Y302/01001

  Alpha-amylase (3.2.1.1) · CPC title

• C12N9/2417 Primary

  from microbiological source · CPC title

• C11D3/386 Primary

  Preparations containing enzymes {, e.g. protease or amylase} · CPC title

• C11D11/0023

  Chemistry & Metallurgy · mapped topic

• C11D3/38609 Primary

  Protease or amylase in solid compositions only · CPC title