Processes for Filtering Liquids Using Single Pass Tangential Flow Filtration Systems And Tangential Flow Filtration Systems With Recirculation of Retentate

Patent metadata

Field	Value
Publication number	US-2019282937-A1
Application number	US-201916427942-A
Country	US
Kind code	A1
Filing date	May 31, 2019
Priority date	Aug 29, 2014
Publication date	Sep 19, 2019
Grant date	—

Abstract

Methods of filtering a liquid feed are disclosed. In one version, the method comprises passing a liquid feed through a single pass tangential flow filtration (SPTFF) system and recovering the retentate and permeate from the system in separate containers without recirculation through the SPTFF system. In another version, the method of filtering a liquid feed, comprises passing a liquid feed through a tangential flow filtration (TFF) system, recovering permeate and a portion of the retentate from the system in separate containers without recirculation through the TFF system, and recirculating the remainder of the retentate through the TFF system at least once. The methods can be performed using an SPTFF or a TFF system that comprises manifold segments to serialize the flow path of the feed and retentate without requiring diverter plates.

First claim

What is claimed is:

1 . A method of filtering a liquid feed, comprising:
a) passing a liquid feed through a single pass tangential flow filtration (SPTFF) system that consists of one filtration module comprising:
a single manifold segment that is a block comprising a first manifold for receiving and carrying the feed into the filtration module, a second manifold for receiving and carrying retentate out of the filtration module, and a third manifold for receiving and carrying permeate through the filtration module, to create a serial liquid flow path for feed, retentate and permeate through the system;
one or more TFF capsules that are positioned on one or both faces of, and are fluidly connected to, the manifold segment;
a feed inlet; and
a retentate outlet,
the system having a liquid flow path that is parallel into the capsules and serial through the manifold segment; and b) recovering the retentate and permeate from the system in separate containers without recirculation through the SPTFF system, thereby filtering the liquid feed.

2 . The method of claim 1 , wherein the filtration area for the filtration module is 20 m 2 or less.

3 . The method of claim 1 , further comprising a diafiltration step, wherein the diafiltration step includes concentration and dilution steps.

4 . The method of claim 3 , wherein the SPTFF system further comprises a diafiltration solution reservoir for diafiltration solution and a conduit for delivering diafiltration solution to a feed reservoir.

5 . The method of claim 1 , wherein the SPTFF system further comprises one or more of:
sampling ports, a T-line for in-line buffer addition, a pressure sensor and a diaphragm for a pressure sensor.

6 . The method of claim 3 , wherein SPTFF system includes a diafiltrate solution pump having multiple pump heads, each containing diafiltrate solution addition and permeate removal flow lines.

7 . The method of claim 1 , wherein the filtration area for the filtration module is 10 m 2 ; 1 m 2 ; or 0.1 m 2 .

8 . A method of filtering a liquid feed, comprising:
a) passing a liquid feed through a tangential flow filtration (SPTFF) system that consists of one filtration module comprising:
a single manifold segment that is a block comprising a first manifold for receiving and carrying the feed into the filtration module, a second manifold for receiving and carrying retentate out of the filtration module, and a third manifold for receiving and carrying permeate through the filtration module, to create a serial liquid flow path for feed, retentate and permeate through the system;
one or more TFF capsules that are positioned on one or both faces of, and are fluidly connected to, the manifold segment;
a feed inlet; and
a retentate outlet,
the system having a liquid flow path that is parallel into the capsules and serial through the manifold segment; b) recovering permeate in a permeate container and a portion of the retentate from the system in a retentate container without recirculation through the TFF system; and c) recirculating the remainder of the retentate through the TFF system at least once, thereby filtering the liquid feed.

9 . The method of claim 8 , wherein 10% or less of the retentate is recirculated after the first pass through the TFF system.

10 . The method of claim 8 , wherein the TFF system includes a valve or flow meter that is positioned on the retentate outlet or conduit carrying retentate from the system to the retentate container to control the amount of retentate that is recirculated.

11 . The method of claim 8 , wherein retentate is recirculated back to a feed container in the TFF system or back to a feed line after a feed pump in the TFF system.

12 . The method of claim 8 , wherein the filtration area for the filtration module is 20 m 2 or less.

13 . The method of claim 8 , further comprising a diafiltration step, wherein the diafiltration step includes concentration and dilution steps.

14 . The method of claim 13 , wherein the SPTFF system further comprises a diafiltration solution reservoir for diafiltration solution and a conduit for delivering diafiltration solution to a feed reservoir.

15 . The method of claim 8 , wherein the SPTFF system further comprises one or more of: sampling ports, a T-line for in-line buffer addition, a pressure sensor and a diaphragm for a pressure sensor.

16 . The method of claim 13 , wherein SPTFF system includes a diafiltrate solution pump having multiple pump heads, each containing diafiltrate solution addition and permeate removal flow lines.

Assignees

• Emd Millipore Corp

Inventors

• Steen Jonathan
• Kozlov Mikhail
• Lutz Herbert
• Parrella Joseph
• Goodrich Elizabeth M
• Raghunath Bala

Classifications

• B01D61/145 Primary

  Ultrafiltration · CPC title

• B01D61/147

  Microfiltration · CPC title

• B01D2313/125

  Discharge manifolds · CPC title

• B01D2313/44

  Cartridge types · CPC title

• B01D2313/105

  Supply manifolds · CPC title