Microfluidic device having injection-molded fluidics layer, and method of making same

Patent metadata

Field	Value
Publication number	US-2019257827-A1
Application number	US-201916296618-A
Country	US
Kind code	A1
Filing date	Mar 8, 2019
Priority date	Dec 1, 2015
Publication date	Aug 22, 2019
Grant date	—

Abstract

A microfluidic device for conducting a fluid assay includes an injection-molded (or “molded”) fluidics layer having at least one microfluidic channel configured to allow assay fluids to flow there-along, the channel having channel side walls, a channel bottom, and a channel 3D geometry, and the fluidics layer being made from injection-molded liquid silicone (or PDMS). Having the fluidics layer made from injection molded liquid silicone enables smaller-sized channel features, such as microfluidic valves and pistons, smaller channel dimensions and spacing (providing smaller device footprint, higher device capacity and other benefits), and various geometries for the channels and channel features.

First claim

What is claimed is:

1 . A microfluidic device for conducting a fluid assay, comprising:
a fluidics layer having at least one micro-fluidic channel configured to allow assay fluids to flow there-along, the channel having channel side walls and a channel bottom, the channel having a channel geometry; and the fluidics layer being made from injection-molded clear liquid silicone.

2 . The microfluidic device of claim 1 , wherein the channel geometry comprises at least one of: rounded bottom, rounded sides, and spherical shape.

3 . The microfluidic device of claim 1 , wherein the microfluidic channel having a channel height and channel width and having at least one microfluidic valve seat disposed on the channel bottom, the valve seat having a geometry comprising at least one of: oval, rounded, square, and flat-top triangle.

4 . The microfluidic device of claim 1 , wherein the valve seat having a height that is below the channel height.

5 . The microfluidic device of claim 1 , wherein the channel has a channel width of less than about 125 microns.

6 . The microfluidic device of claim 1 , wherein there are a plurality of microfluidic channels, at least two of the channels having a spacing distance therebetween of about 1.5 mm to about 200 microns.

7 . The microfluidic device of claim 1 , wherein the microfluidic channel has at least one channel feature portion, comprising a valve feature or a piston feature.

8 . The microfluidic device of claim 1 , wherein the microfluidic channel has at least one channel feature portion, comprising a valve feature or a piston feature, and the feature portion having a depth or geometry that is different from the rest of the channel.

9 . The microfluidic device of claim 1 , wherein the microfluidic channel has at least one feature portion, comprising a valve feature or a piston feature, and the feature portion having a depth or geometry that is different from the rest of the channel.

10 . The microfluidic device of claim 1 , wherein at least one of the channel width and the channel depth being varying in value.

11 . The microfluidic device of claim 10 , wherein the channel is configured as a channel-type filter which traps particles or cells.

12 . The microfluidic device of claim 1 , wherein the injection-molded liquid silicone comprises injection-molded clear liquid polydimethylsiloxane (PDMS).

13 . The microfluidic device of claim 1 , further comprising at least one flow element in fixed position in the channel and a flexible membrane layer bonded to a top side of the fluidics layer, a portion of the flexible membrane layer configured to deflect into or away from the channel due to pneumatic pressure applied to one side of the membrane portion to actuate at least one of a valve and a piston.

14 . A method of making a microfluidic device for conducting a fluid assay, comprising:
injecting liquid silicone into a mold template having at least one microfluidic channel and at least one channel feature disposed in the mold template, to form a fluidics layer; removing the fluidics layer from the mold template; and placing the fluidics layer on a non-stick surface during curing of the fluidics layer, the non-stick surface allowing the fluidics layer to shrink as it cures, thereby ensuring final dimensions of the fluidics layer meets predetermined tolerances.

15 . The method of claim 14 , further comprising:
inserting at least one flow element into the channel; and attaching a flexible membrane layer to a top side of the fluidics layer.

16 . The method of claim 15 , further comprising attaching a relatively rigid backing surface to a bottom side of the fluidics layer before the inserting step.

17 . The method of claim 16 , further comprising attaching a relatively rigid reservoir layer to the membrane layer after the step of attaching the membrane layer to the fluidics layer, the reservoir layer having pneumatic channels disposed on an underside of the relatively rigid plastic reservoir layer, the flexible membrane layer configured to deflect due to pneumatic pressure applied along the pneumatic channels to one side of the membrane portion to actuate at least one of a valve and a piston.

18 . The method of claim 17 , wherein the relatively rigid backing surface is removable, and further comprising removing the relatively rigid backing surface from the bottom side of the fluidics layer.

19 . The method of claim 14 , wherein the fluidics layer has a thickness of about 1.1 mm.

20 . The method of claim 14 , wherein the at least one feature is a valve feature or a piston feature.

21 . The method of claim 14 , wherein the non-stick surface comprises surface-coated Teflon.

22 . The method of claim 14 , wherein the liquid silicone comprises clear liquid PDMS.

23 . A microfluidic device for conducting a fluid assay, comprising:
an injection-molded fluidics layer having at least one microfluidic channel configured to allow assay fluids to flow there-along, the fluidics layer being made from injection-molded liquid silicone; at least one flow element in fixed position in the channel; and a flexible membrane layer bonded to a top side of the fluidics layer, a portion of the flexible membrane layer configured to deflect into or away from the channel due to pneumatic pressure applied to one side of the membrane portion to actuate at least one of a valve and a piston.

24 . The microfluidic device of claim 23 , wherein the channel has a channel height and a channel bottom, and has at least one microfluidic valve seat in the channel that engages with the flexible membrane to form the valve, the valve seat having a height that is below the channel height.

Assignees

• ProteinSimple

Inventors

• Stanwood Charles
• Daigneault Noel
• Branciforte Jeffrey

Classifications

• B01L2300/021

  Identification, e.g. bar codes · CPC title

• B01L2400/0481

  squeezing of channels or chambers · CPC title

• B01L2300/0816

  Cards, e.g. flat sample carriers usually with flow in two horizontal directions · CPC title

• B01L2400/0655

  pinch valves · CPC title

• B01L2300/0867

  Multiple inlets and one sample wells, e.g. mixing, dilution · CPC title