Novel clostridium sp. strain producing hexanoic acid in high yield and method for producing hexanoic acid using the same

Patent metadata

Field	Value
Publication number	US-2019226036-A1
Application number	US-201916253581-A
Country	US
Kind code	A1
Filing date	Jan 22, 2019
Priority date	Jan 23, 2018
Publication date	Jul 25, 2019
Grant date	—

Abstract

The present disclosure relates to a Clostridium sp. JS66 strain producing metabolites having 4 to 6 carbon atoms in a high yield. The strain produces metabolites having 6 carbon atoms in a significantly high yield while reducing the production of acetic acid and ethanol as by-products.

First claim

What is claimed is:

1 . A Clostridium sp. strain of accession No. KCTC13355BP producing metabolites having 4 to 6 carbon atoms.

2 . The Clostridium sp. strain according to claim 1 ,
wherein the metabolites comprise at least one of butyric acid, butanol, hexanoic acid and hexanol.

3 . The Clostridium sp. strain according to claim 1 ,
wherein the Clostridium sp. strain produces the metabolites using at least one of glucose, fructose, galactose, xylose, arabinose, mannose, cellobiose, sucrose, carbon monoxide, and carbon dioxide as a carbon source.

4 . The Clostridium sp. strain according to claim 3 ,
wherein the Clostridium sp. strain produces the metabolites using a mixed gas comprising hydrogen and at least one of carbon monoxide and carbon dioxide as a substrate.

5 . The Clostridium sp. strain according to claim 1 ,
wherein the optimal culture temperature is 25° C. to 30° C.

6 . A method for producing metabolites having 4 to 6 carbon atoms, comprising supplying Clostridium sp. strain of accession No. KCTC13355BP to a substrate comprising a carbon source and culturing the strain.

7 . The method for production according to claim 6 ,
wherein the culture is carried out at 25° C. to 30° C.

8 . The method for production according to claim 6 ,
wherein the culture is carried out at pH 5 to pH 8.

9 . The method for production according to claim 6 ,
wherein the culture is carried out under anaerobic conditions.

10 . The method for production according to claim 6 ,
wherein the substrate comprises at least one of glucose, fructose, galactose, xylose, arabinose, mannose, cellobiose, and sucrose.

11 . The method for production according to claim 6 ,
wherein the substrate comprises at least one of carbon monoxide and carbon dioxide.

12 . The method for production according to claim 11 ,
wherein the substrate further comprises hydrogen.

13 . The method for production according to claim 11 ,
wherein the culture is carried out at a total gas pressure of 3 bar or less.

14 . The method for production according to claim 6 ,
wherein the culture is carried out in a medium containing yeast extract.

15 . The method for production according to claim 14 ,
wherein the medium contains 0.1 g/L to 10 g/L of yeast extract.

16 . A method for fixing carbon monoxide and carbon dioxide, comprising supplying a mixed gas comprising carbon monoxide and carbon dioxide to a Clostridium sp. strain of accession No. KCTC13355BP to culture it.

17 . The method for fixing carbon monoxide and carbon dioxide according to claim 16 , comprising producing metabolites having 4 to 6 carbon atoms from carbon monoxide and carbon dioxide.

Assignees

• Korea Inst Sci & Tech

Inventors

• Um Youngsoon
• Kim Joongsuk
• Lee Sun-Mi
• Gong Gyeongtaek

Classifications

• C12N1/20 Primary

  Bacteria; Culture media therefor · CPC title

• C12P7/40

  containing a carboxyl group {including Peroxycarboxylic acids} · CPC title

• C12P7/16

  Butanols · CPC title

• C12P7/52 Primary

  Propionic acid; Butyric acids · CPC title

• C12P7/04

  acyclic · CPC title