Fermentation product of punica granatum and uses thereof

Patent metadata

Field	Value
Publication number	US-2019224259-A1
Application number	US-201816206123-A
Country	US
Kind code	A1
Filing date	Nov 30, 2018
Priority date	Jan 19, 2018
Publication date	Jul 25, 2019
Grant date	—

Abstract

The present disclosure provides a fermentation product of Punica granatum and methods for regulating expression of MMP gene, TIMP gene and COL4A4 gene, promoting collagen production, and anti-aging by using the fermentation product of Punica granatum.

First claim

What is claimed is:

1 . A fermentation product of Punica granatum obtained by a process comprising the following steps:
(a) extracting the Punica granatum with water to obtain a Punica granatum extract; and (b) sequentially fermenting the Punica granatum extract with Saccharomyces cerevisiae and Lactobacillus plantarum to obtain a first fermentation product of Punica granatum; wherein the Saccharomyces cerevisiae has a concentration ranging from 0.01% to 0.5%, and the Lactobacillus plantarum has a concentration ranging from 0.01% to 0.25%.

2 . The fermentation product of Punica granatum according to claim 1 , wherein a fermentation time of the Saccharomyces cerevisiae ranges from 1 to 2 days, and a fermentation time of the Lactobacillus plantarum ranges from 1 to 3 days.

3 . The fermentation product of Punica granatum according to claim 1 , wherein the first fermentation product of Punica granatum is further fermented with Acetobacter aceti to obtain a second fermentation product of Punica granatum.

4 . The fermentation product of Punica granatum according to claim 3 , wherein the Acetobacter aceti has a concentration ranging from 1% to 20%.

5 . The fermentation product of Punica granatum according to claim 4 , wherein a fermentation time of the Acetobacter aceti ranges from 14 to 21 days.

6 . The fermentation product of Punica granatum according to claim 1 , wherein an extraction temperature of the process ranges from 50° C. to 100° C., and an extraction time of the process ranges from 0.5 to 3 hours.

7 . A method for regulating expression of matrix metalloproteinase (MMP) gene, tissue inhibitor of matrix metalloproteinase (TIMP) gene, and collagen type IV alpha 4 chain (COL4A4) gene, comprising administering to a subject in need thereof a composition comprising an effective amount of the first fermentation product of Punica granatum according to claim 1 .

8 . The method according to claim 7 , wherein the first fermentation product of Punica granatum is further fermented with Acetobacter aceti to obtain a second fermentation product of Punica granatum.

9 . The method according to claim 7 , wherein the composition is in the form of a pharmaceutical composition, a food product, or a cosmetic composition.

10 . The method according to claim 7 , wherein the MMP gene is MMP2 gene.

11 . The method according to claim 7 , wherein the TIMP gene is TIMP1 gene.

12 . A method for promoting collagen production and anti-aging, comprising administering to a subject in need thereof a composition comprising an effective amount of the first fermentation product of Punica granatum according to claim 1 .

13 . The method according to claim 12 , wherein the composition is in the form of the cosmetic composition.

14 . The method according to claim 12 , wherein the first fermentation product of Punica granatum is further fermented with Acetobacter aceti to obtain a second fermentation product of Punica granatum.

15 . The method according to claim 14 , wherein the composition is in the form of the food product.

Assignees

• Tci Co Ltd

Inventors

• Lin Yung-Hsiang
• Chuang Wei Hsiu

Classifications

• A61K8/9789

  Magnoliopsida [dicotyledons] · CPC title

• A61K2800/85

  Products or compounds obtained by fermentation, e.g. yoghurt, beer, wine · CPC title

• A61Q19/08

  Anti-ageing preparations · CPC title

• A23L33/105

  Plant extracts, their artificial duplicates or their derivatives · CPC title

• A23V2250/21

  Plant extracts · CPC title