Polypeptides Having Xylanase Activity and Polynucleotides Encoding Same

Patent metadata

Field	Value
Publication number	US-2019194635-A1
Application number	US-201615780299-A
Country	US
Kind code	A1
Filing date	Dec 16, 2016
Priority date	Dec 18, 2015
Publication date	Jun 27, 2019
Grant date	—

Abstract

The present invention relates to polypeptides having xylanase activity and polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides. The invention also relates to compositions comprising the polypeptides of the invention and the use of the polypeptides of the invention to solubilise xylan and in animal feed.

First claim

1 - 25 . (canceled)

26 . A method of solubilising xylan from plant based material, comprising treating plant based material with a GH30 subfamily 8 polypeptide having xylanase activity, wherein the GH30 subfamily 8 polypeptide having xylanase activity is selected from the group consisting of:
(a) a polypeptide having at least 80% sequence identity to the polypeptide of SEQ ID NO: 60; (b) a polypeptide having at least 80% sequence identity to the polypeptide of SEQ ID NO: 6; (c) a polypeptide having at least 92% sequence identity to the polypeptide of SEQ ID NO: 12; (d) a polypeptide having at least 80% sequence identity to the polypeptide of SEQ ID NO: 18; (e) a polypeptide having at least 80% sequence identity to the polypeptide of SEQ ID NO: 24; (f) a polypeptide having at least 80% sequence identity to the polypeptide of SEQ ID NO: 30; (g) a polypeptide having at least 80% sequence identity to the polypeptide of SEQ ID NO: 36; (h) a polypeptide having at least 85% sequence identity to the polypeptide of SEQ ID NO: 42; (i) a polypeptide having at least 80% sequence identity to the polypeptide of SEQ ID NO: 48; (j) a polypeptide having at least 80% sequence identity to the polypeptide of SEQ ID NO: 54; (k) a variant of the polypeptide of SEQ ID NO: 60, SEQ ID NO: 6, SEQ ID NO: 18, SEQ ID NO: 24, SEQ ID NO: 30, SEQ ID NO: 36, SEQ ID NO: 42, SEQ ID NO: 48 or SEQ ID NO: 54 comprising one or more amino acid substitutions, and/or one or more amino acid deletions, and/or one or more amino acid insertions or any combination thereof in 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49 or 50 positions; (l) a variant of the polypeptide of SEQ ID NO: 12 comprising one or more amino acid substitutions, and/or one or more amino acid deletions, and/or one or more amino acid insertions or any combination thereof in 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38 or 39 positions; (m) a polypeptide comprising the polypeptide of (a), (b), (c), (d), (e), (f), (g), (h), (i), (j), (k) or (l) and a N-terminal and/or C-terminal His-tag and/or HQ-tag; and (n) a fragment of a polypeptide of (a), (b), (c), (d), (e), (f), (g), (h), (i), (j), (k), (l) or (m) having at least 90% of the length of the mature polypeptide.

27 . The method of claim 26 , wherein the polypeptide comprises the motif YXVWVY[I/L]RRXYG (SEQ ID NO: 1).

28 . The method of claim 26 , wherein the polypeptide comprises or consists of amino acids 1 to 557 of SEQ ID NO: 60, amino acids 1 to 569 of SEQ ID NO: 63, amino acids 1 to 382 of SEQ ID NO: 6, amino acids 1 to 390 of SEQ ID NO: 9, amino acids 1 to 391 of SEQ ID NO: 12, amino acids 1 to 399 of SEQ ID NO: 15, amino acids 1 to 383 of SEQ ID NO: 18, amino acids 1 to 391 of SEQ ID NO: 21, amino acids 1 to 565 of SEQ ID NO: 24, amino acids 1 to 573 of SEQ ID NO: 27, amino acids 1 to 396 of SEQ ID NO: 30, amino acids 1 to 404 of SEQ ID NO: 33, amino acids 1 to 392 of SEQ ID NO: 36, amino acids 1 to 400 of SEQ ID NO: 39, amino acids 1 to 413 of SEQ ID NO: 42, amino acids 1 to 421 of SEQ ID NO: 45, amino acids 1 to 398 of SEQ ID NO: 48, amino acids 1 to 406 of SEQ ID NO: 51, amino acids 1 to 382 of SEQ ID NO: 54, amino acids 1 to 390 of SEQ ID NO: 57.

29 . The method of claim 26 , wherein the percentage of solubilized xylan is at least 4% when the method is performed under the reaction conditions 20 μg GH30 polypeptide per gram defatted destarched maize (DFDSM) and incubation at 40° C., pH 5 for 2.5 hours.

30 . The method of claim 26 , wherein the plant based material is from the sub-family Panicoideae.

31 . A granule comprising one or more GH30 subfamily 8 polypeptides having xylanase activity, wherein the GH30 subfamily 8 polypeptide having xylanase activity is selected from the group consisting of:
(a) a polypeptide having at least 80% sequence identity to the polypeptide of SEQ ID NO: 60; (b) a polypeptide having at least 80% sequence identity to the polypeptide of SEQ ID NO: 6; (c) a polypeptide having at least 92% sequence identity to the polypeptide of SEQ ID NO: 12; (d) a polypeptide having at least 80% sequence identity to the polypeptide of SEQ ID NO: 18; (e) a polypeptide having at least 80% sequence identity to the polypeptide of SEQ ID NO: 24; (f) a polypeptide having at least 80% sequence identity to the polypeptide of SEQ ID NO: 30; (g) a polypeptide having at least 80% sequence identity to the polypeptide of SEQ ID NO: 36; (h) a polypeptide having at least 85% sequence identity to the polypeptide of SEQ ID NO: 42; (i) a polypeptide having at least 80% sequence identity to the polypeptide of SEQ ID NO: 48; (j) a polypeptide having at least 80% sequence identity to the polypeptide of SEQ ID NO: 54; (k) a variant of the polypeptide of SEQ ID NO: 60, SEQ ID NO: 6, SEQ ID NO: 18, SEQ ID NO: 24, SEQ ID NO: 30, SEQ ID NO: 36, SEQ ID NO: 42, SEQ ID NO: 48 or SEQ ID NO: 54 comprising one or more amino acid substitutions, and/or one or more amino acid deletions, and/or one or more amino acid insertions or any combination thereof in 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49 or 50 positions; (l) a variant of the polypeptide of SEQ ID NO: 12 comprising one or more amino acid substitutions, and/or one or more amino acid deletions, and/or one or more amino acid insertions or any combination thereof in 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38 or 39 positions; (m) a polypeptide comprising the polypeptide of (a), (b), (c), (d), (e), (f), (g), (h), (i), (j), (k) or (l) and a N-terminal and/or C-terminal His-tag and/or HQ-tag; and (n) a fragment of a polypeptide of (a), (b), (c), (d), (e), (f), (g), (h), (i), (j), (k), (l) or (m) having at least 90% of the length of the mature polypeptide.

32 . The granule of claim 31 , wherein the polypeptide comprises the motif YXVWVY[I/L]RRXYG (SEQ ID NO: 1).

33 . The granule of claim 31 , wherein the granule comprises one or more formulating agents.

34 . The granule of claim 31 , wherein the granule comprises a core particle and one or more coatings.

35 . The granule of claim 31 , wherein the polypeptide solubilizes at least 4% xylan from defatted destarched maize (DFDSM).

36 . An isolated polypeptide having xylanase activity, selected from the group consisting of:
(a) a polypeptide having at least 80% sequence identity to the polypeptide of SEQ ID NO: 6; (b) a polypeptide having at least 92% sequence identity to the polypeptide of SEQ ID NO: 12; (c) a polypeptide having at least 86% sequence identity to the polypeptide of SEQ ID NO: 30; (d) a polypeptide having at least 90% sequence identity to the polypeptide of SEQ ID NO: 36; (e) a polypeptide having at least 85% sequence identity to the polypeptide of SEQ ID NO: 42; (f) a polypeptide having at least 80% sequence identity to the polypeptide of SEQ ID NO: 48; (g) a polypeptide having at least 80% sequence identity to the polypeptide of SEQ ID NO: 54; (h) a polypeptide encoded by a polynucleotide that hybridizes under high stringency conditions, or very high stringency conditions with
(i) the mature polypeptide coding sequence of SEQ ID NO: 4,
(ii) the mature polypeptide coding sequence of SEQ ID NO: 10,
(iii) the mature polypeptide coding sequence of SEQ ID NO: 28,
(iv) the mature pol

Assignees

• Novozymes As

Inventors

• Nymand-Grarup Soren
• Pedersen Ninfa Rangel
• Gonzalez Lorena Palmen
• Pettersson Dan
• Eklof Jens Magnus
• Blom Charlotte
• Salomon Jesper

Classifications

• A23K20/189

  Enzymes · CPC title

• C12N9/2482 Primary

  Endo-1,4-beta-xylanase (3.2.1.8) · CPC title

• A23K20/174

  Vitamins · CPC title

• A23K50/30

  for swines · CPC title

• C12Y302/01008

  Endo-1,4-beta-xylanase (3.2.1.8) · CPC title