Synergistic antimicrobial effect

Patent metadata

Field	Value
Publication number	US-2019192589-A1
Application number	US-201916292246-A
Country	US
Kind code	A1
Filing date	Mar 4, 2019
Priority date	Apr 8, 2011
Publication date	Jun 27, 2019
Grant date	—

Abstract

The present invention is related to the field of bioprotection, in particular to an antimicrobial composition comprising a Lactobacillus rhamnosus strain and/or a Lactobacillus paracasei strain. Furthermore, the present invention concerns uses for such an antimicrobial composition, food, feed and pharmaceutical products comprising such an antimicrobial composition, a method of manufacturing such food, feed and pharmaceutical products and a method for reducing the content of unwanted microorganisms of such food, feed and pharmaceutical products.

First claim

1 - 24 . (canceled)

25 . An antimicrobial composition comprising (i) at least one strain of Lactobacillus paracasei selected from Lactobacillus paracasei CHCC12777 deposited with the German Collection of Microorganisms and Cell Cultures (DSMZ) under accession no. DSM24651 or a mutant strain thereof, wherein the mutant strain is obtained by using the deposited strain as a starting material and (ii) a cryoprotectant effective to improve the cold tolerance of the microorganisms.

26 . An antimicrobial composition according to claim 25 , wherein the at least one strain of Lactobacillus paracasei comprises Lactobacillus paracasei CHCC12777 deposited with the Gelman Collection of Microorganisms and Cell Cultures (DSMZ) under accession no. DSM24651.

27 . An antimicrobial composition according to claim 25 , wherein the cryoprotectant comprises one or more selected from mannitol, sorbitol, sodium tripolyphosphate, xylitol, glycerol, raffinose, maltodextrin, erythritol, threitol, trehalose, glucose and fructose.

28 . An antimicrobial composition according to claim 25 , further comprising one or more nutrients.

29 . An antimicrobial composition according to claim 28 , wherein the one or more nutrients are selected from yeast extracts, sugars, and vitamins.

30 . An antimicrobial composition according to claim 25 in frozen, dried, or freeze-dried form having a concentration of viable cells of the at least one strain of Lactobacillus paracasei of from 10 4 to 10 12 colony forming units (cfu) per gram of the composition.

31 . An antimicrobial composition according to claim 25 in liquid form having a concentration of viable cells of the at least one strain of Lactobacillus paracasei of from 10 4 to 10 12 colony forming units (cfu) per ml of the composition.

32 . A pharmaceutical product for treating infections with pathogenic fungi,
comprising an effective amount of the antimicrobial composition according to claim 25 .

33 . A pharmaceutical product according to claim 32 , in a form selected from a capsule, a tablet, a paste, a cream, and an ointment.

34 . A pharmaceutical product according to claim 32 , comprising at least 10 6 cfu/g or at least to 10 6 cfu/ml of the at least one strain of Lactobacillus paracasei.

35 . A food or feed product comprising a food or feed product and an antimicrobially effective amount of the antimicrobial composition according to claim 25 .

36 . A product according to claim 35 , wherein the product is a food product selected from fruits and fruit-derived products, vegetables and vegetable-derived products, grain and grain-derived products, dairy products, meat, poultry, seafood, and mixtures of two or more thereof.

37 . A product according to claim 35 , comprising at least 10 6 cfu/g or at least to 10 6 cfu/ml of the food product, or at least 10 5 cfu/cm 2 of the surface of the product.

38 . A method for manufacturing a food, feed, or pharmaceutical product, comprising adding the antimicrobial composition of claim 25 to a food, feed or pharmaceutical product.

39 . A method according to claim 38 , comprising:
(a) adding the antimicrobial composition during the manufacture of the food, feed, or pharmaceutical product such that the concentration of the at least one strain of Lactobacillus paracasei is at least 1×10 6 cfu/g or at least 1×10 6 cfu/ml of the food, feed or pharmaceutical product, or at least 1×10 5 cfu/cm 2 of the surface of the food, feed or pharmaceutical product; and then (b) controlling the manufacturing parameters during the manufacturing such that the concentration of the at least one strain of Lactobacillus paracasei increases or remains constant.

40 . A method according to claim 38 , wherein step (a) comprises blending the at least one strain of Lactobacillus paracasei into the food, feed, or pharmaceutical product.

41 . A method according to claim 38 , wherein step (a) comprises applying the at least one strain of Lactobacillus paracasei to a surface of the food, feed, or pharmaceutical product.

42 . A method of treating infections with pathogenic fungi, comprising administering a pharmaceutical product according to claim 32 to a subject in need thereof.

43 . A method of inhibiting growth of microorganisms in a food, feed, or pharmaceutical product, comprising adding a composition according to claim 25 to the food, feed, or pharmaceutical product in an amount to yield a concentration of the at least one strain of Lactobacillus paracasei of at least 1×10 6 cfu/g or at least 1×10 6 cfu/ml of the food, feed or pharmaceutical product, or at least 1×10 5 cfu/cm 2 of the surface of the food, feed or pharmaceutical product

Assignees

• Chr Hansen As

Inventors

• Hornbaek Tina
• Lisberg Maike
• Diemer Silja Kej

Classifications

• A61P31/04

  Antibacterial agents · CPC title

• A23C9/1234

  characterised by using a Lactobacillus sp. other than Lactobacillus Bulgaricus, including Bificlobacterium sp. · CPC title

• A61P31/10

  Antimycotics · CPC title

• A61P31/00

  Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics · CPC title

• A01N63/00

  Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates (containing compounds of determined constitution A01N27/00 - A01N59/00; unicellular algae A01N65/03) · CPC title