Gene analysis and generation of stem cell methods and apparatus

Patent metadata

Field	Value
Publication number	US-2019105023-A1
Application number	US-201816211182-A
Country	US
Kind code	A1
Filing date	Dec 5, 2018
Priority date	Sep 5, 2014
Publication date	Apr 11, 2019
Grant date	—

Abstract

A surgical treatment apparatus comprises a waterjet configured to fragment tissue and provide intact cells such as stem cells with the fragmented tissue. The intact cells can be used in one or more of many ways such as for genetic or other testing, and the intact cells can be identified as stem cells. In many embodiments, the intact cells comprise stem cells. In many embodiments, a waterjet is configured to fragment tissue. The fragmented tissue can be collected with a filter having pores sized smaller than the tissue fragments. In many embodiments cavitation with a waterjet is used to fragment the tissue comprising the intact stem cells. The waterjet may comprise a waterjet immersed in a liquid comprising water so as to form a plurality of shedding pulses. The plurality of shedding pulses can be generated with a frequency sufficient to fragment the tissue. The shedding pulses can generate cavitations that fragment the tissue.

First claim

What is claimed is:

1 . An apparatus to remove intact cells from a surgical site of a patient, the apparatus comprising:
a probe insertable to the surgical site, the probe comprising,
a nozzle configured to provide a fluid stream to the surgical site to fragment the tissue, and
a port to receive tissue from the surgical site;
a filter coupled to the port in order to receive fragmented tissue comprising the intact cells from the surgical site; wherein the apparatus comprises a plurality of filters to collect fragmented tissue from a plurality of locations and wherein each of the plurality of filters corresponds to an area of treatment in order to map a sample obtained with a filter to a treatment location.

2 . An apparatus as in claim 1 , wherein the filter comprises a plurality of pores having plurality a pore sizes and wherein the plurality of pore sizes is dimensioned larger than dimensions of the intact cells in order to collect the plurality of fragments comprising the intact cells with the filter.

3 . An apparatus as in claim 1 , wherein the nozzle comprises an inner restricted diameter corresponding to a diameter of the fluid stream released from the nozzle and wherein the nozzle is configured to dissociate the tissue into a plurality of fragments having dimensions sized larger than the inner restricted diameter.

4 . An apparatus as in claim 1 , wherein the nozzle and the port are arranged to provide a slurry to the port, the slurry comprising the plurality of fragments and fluid of the fluid stream.

5 . An apparatus as in claim 3 , wherein nozzle is configured to fragment the tissue with cavitations of a liquid jet.

6 . An apparatus as in claim 4 , wherein nozzle is arranged with the port in order to immerse the liquid jet in a liquid in order to generate a plurality of shedding pulses with the jet immersed in the liquid.

7 . An apparatus as in claim 1 , wherein the nozzle and the port are arranged to provide a closed surgical site within the patient.

8 . An apparatus as in claim 1 , further comprising a first channel extending from a fluid source to the nozzle to generate the fluid stream and a second channel extending from the port toward the filter.

9 . An apparatus as in claim 7 , wherein the first fluid source comprises a first pump connected to the first channel.

10 . An apparatus as in claim 9 , further comprising a second pump connected to the second channel, the first pump comprising a first flow rate, the second pump comprising a second flow rate, the first flow rate similar to the second flow rate in order to remove tissue fragments and fluid at the second rate similar to the first rate of fluid injected into the surgical site with the stream.

11 . An apparatus as in claim 10 , further comprising a fluid reservoir, a channel extending from the fluid reservoir to the surgical site in order to accommodate differences between the first flow rate and second flow rate and inhibit changes to a volume of a closed surgical site within the patient.

12 . An apparatus as in claim 1 , wherein the fluid stream comprises one or more of a liquid or a gas.

13 . An apparatus as in claim 1 , wherein the fluid stream comprises a liquid stream, the liquid stream comprising one or more of water or saline.

14 . An apparatus as in claim 1 , wherein the cells comprise cells of a glandular tissue of an organ.

15 . The apparatus of claim 1 , wherein a flow rate is within a range from about 10 ml/min to about 500 ml/min, or wherein the flow rate is within a range from about 50 to 250 ml/min.

16 . The apparatus of claim 1 , wherein an internal nozzle diameter is within a range from about 50 um to 250 um, or wherein the internal nozzle diameter is within a range from about 120 to 150 um.

17 . The apparatus of claim 1 , wherein an angular velocity of the nozzle rotating around an elongate axis of the probe is within a range from about 10 degrees per second to 2000 degrees per second or wherein the angular velocity of the nozzle is within a range from about 180 to 900 degrees per second.

18 . The apparatus of claim 1 , wherein a longitudinal velocity of the nozzle along an elongate axis of the probe is within a range from about 0.01 mm/second to about 50 mm/second, or within a range from about 0.1 mm/second to about 5 mm/second

19 . The apparatus of claim 1 , wherein a time of the treatment with the waterj et is within a range from about 0.1 minutes to about 60 minutes or wherein the time is within a range from about 1 to about 10 minutes.

20 . The apparatus of claim 1 , wherein the rotation treatment angles of the probe about the elongate axis are within a range from about 0 to 360 degrees, or within a range from about 30 to 300 degrees.

21 . The apparatus of claim 1 , wherein a longitudinal length of the treatment is within a range from about 0.1 mm to about 300 mm, or within a range from about 1 mm to about 70 mm.

22 . The apparatus of claim 1 , wherein a pump to draw fluid from the surgical site comprises one or more of a vacuum pump or a flow pump.

Assignees

• Procept Biorobotics Corp

Inventors

• Aljuri Nikolai
• Mantri Surag

Classifications

• A61B10/0275

  with sample notch, e.g. on the side of inner stylet · CPC title

• A61B2017/32032

  using cavitation of the fluid · CPC title

• A61B10/0241

  for prostate · CPC title

• G01N1/00

  Sampling; Preparing specimens for investigation · CPC title

• A61B2217/005

  with suction drainage system · CPC title