Enzymatically produced cellulose

Patent metadata

Field	Value
Publication number	US-2019100782-A1
Application number	US-201816191483-A
Country	US
Kind code	A1
Filing date	Nov 15, 2018
Priority date	Dec 23, 2014
Publication date	Apr 4, 2019
Grant date	—

Abstract

Enzymatic reactions are disclosed herein comprising water, glucose-1-phosphate, cellodextrin, and at least one cellodextrin phosphorylase enzyme comprising an amino acid sequence that is at least 90% identical to SEQ ID NO:2 or SEQ ID NO:6. These reactions produce a low molecular weight, insoluble cellulose with enhanced features.

First claim

What is claimed is:

1 . An enzymatic reaction comprising water, glucose-1-phosphate, cellodextrin, and a cellodextrin phosphorylase enzyme comprising an amino acid sequence that is at least 90% identical to SEQ ID NO:2 or SEQ ID NO:6,
wherein the cellodextrin phosphorylase enzyme synthesizes insoluble cellulose.

2 . The enzymatic reaction of claim 1 , wherein said cellulose has a weight-average degree of polymerization (DP w ) of 10 to 1000.

3 . The enzymatic reaction of claim 1 , wherein said cellulose has a weight-average degree of polymerization (DP w ) of 10 to 30.

4 . The enzymatic reaction of claim 1 , wherein the cellodextrin comprises cellobiose.

5 . A method for producing insoluble cellulose, said method comprising:
a) contacting at least water, glucose-1-phosphate, cellodextrin, and a cellodextrin phosphorylase enzyme comprising an amino acid sequence that is at least 90% identical to SEQ ID NO:2 or SEQ ID NO:6, wherein insoluble cellulose is produced; and b) optionally, isolating the insoluble cellulose produced in step (a).

6 . The method of claim 5 , wherein the cellulose produced in step (a) has a weight-average degree of polymerization (DP w ) of about 10 to about 1000.

7 . The method of claim 5 , wherein the cellulose produced in step (a) has a weight-average degree of polymerization (DP w ) of about 10 to about 30.

8 . The method of claim 5 , wherein the cellulose produced in step (a) has a cellulose II crystal structure.

9 . The method of claim 5 , wherein the cellodextrin comprises cellobiose.

10 . The method of claim 5 , wherein said glucose-1-phosphate is provided in step (a) by providing a second reaction, wherein the products of the second reaction comprise glucose-1-phosphate.

11 . The method of claim 10 , wherein the second reaction produces glucose-1-phosphate by:
(i) contacting water, inorganic phosphate, starch, a starch phosphorylase, and optionally a starch debranching enzyme such as a pullulanase or isoamylase; (ii) contacting water, inorganic phosphate, sucrose, and a sucrose phosphorylase enzyme; or (iii) contacting water, inorganic phosphate, cellulosic biomass, an endoglucanase, a cellodextrin phosphorylase, and optionally, a lytic polysaccharide monooxygenase and/or a cellobiohydrolase.

12 . The method of claim 10 , wherein the second reaction is provided in the same vessel in which step (a) is performed, and wherein the second reaction is performed before and/or continuously with step (a).

Assignees

• Du Pont

Inventors

• Behabtu Natnael
• Poulose Ayrookaran J
• Yu Zheyong
• Zhang Zhenghong

Classifications

• A61P17/16

  Emollients or protectives, e.g. against radiation · CPC title

• C12Y204/01049

  Cellodextrin phosphorylase (2.4.1.49) · CPC title

• C12P19/04 Primary

  Polysaccharides, i.e. compounds containing more than five saccharide radicals attached to each other by glycosidic bonds · CPC title

• C09D101/02

  Cellulose; Modified cellulose · CPC title

• C08L1/02

  Cellulose; Modified cellulose · CPC title