Method, device and non-transitory computer-readable medium for localizing individual emitters in a sample
US-2024046595-A1 · Feb 8, 2024 · US
US2019025492A1 · US · A1
| Field | Value |
|---|---|
| Publication number | US-2019025492-A1 |
| Application number | US-201716069715-A |
| Country | US |
| Kind code | A1 |
| Filing date | Jan 27, 2017 |
| Priority date | Jan 29, 2016 |
| Publication date | Jan 24, 2019 |
| Grant date | — |
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A high-divergence-angle optical fiber apparatus is disclosed that includes a multimode optical fiber having a distal end and a divergence angle θ′. A light-redirecting structure is operably disposed at the distal end and consists of an array of between 1 and 10 layers of fused glass microspheres. The light-redirecting structure defines a divergence angle θ, wherein θ≥2θ′. A light source system that utilizes the high-divergence-angle optical fiber apparatus is also disclosed.
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What is claimed is: 1 . An high-divergence-angle (HDA) optical fiber apparatus, consisting of: a multimode optical fiber having a glass core, a lower-index glass cladding surrounding the glass core, a proximal end and a distal end, wherein the optical fiber has a divergence angle θ′; a light-redirecting structure operably disposed at the distal end and consisting of an array of fused glass microspheres having diameters in the range from 3 microns to 25 microns wherein the array is fused to the distal end and has between 1 layer and 10 layers of microspheres; and wherein the light-redirecting structure defines a divergence angle θ, wherein θ≥2θ′. 2 . The HDA optical fiber apparatus according to claim 1 , wherein the optical fiber has a core diameter D 1 of nominally 40 microns and a cladding outer diameter D 2 of nominally 50 microns. 3 . The HDA optical fiber apparatus according to claim 1 , wherein the glass microspheres, the glass core and the glass cladding are each made of a silica-based glass. 4 . The HDA optical fiber apparatus according to claim 1 , wherein the array has between 1 layer and 6 layers of the microspheres. 5 . The HDA optical fiber apparatus according to claim 1 , wherein the microspheres are solid. 6 . The HDA optical fiber apparatus according to claim 1 , wherein θ≥3θ′. 7 . The HDA optical fiber apparatus according to claim 1 , wherein the core has a diameter D 1 and includes adjacent the distal end a widened core section that has a diameter D 1 ′ that is at least 5% greater than the core diameter D 1 . 8 . The HDA optical fiber apparatus according to claim 1 , wherein the distal end of the optical fiber is curved. 9 . The HDA optical fiber apparatus according to claim 1 , wherein the distal end of the optical fiber includes depressions and protrusions that have a size substantially the same as the glass microspheres. 10 . The HAD optical fiber apparatus according to claim 1 , wherein the fused microspheres define air-filled interstices within the light-redirecting structure. 11 . A light source system, comprising: the HDA optical fiber apparatus according to claim 1 ; and a light source optically coupled to the proximal end of the optical fiber and that includes a light emitter that emits light that is coupled into the optical fiber, wherein the light is emitted from the light-redirecting structure as divergent light over the second divergence angle θ. 12 . The light source system according to claim 11 , wherein the light emitter emits non-polarized visible light in the range from 440 nm to 650 nm. 13 . A method of forming a high-divergence-angle (HDA) optical fiber apparatus using a multimode optical fiber having a glass core, a lower-index glass cladding surrounding the glass core, a proximal end and a distal end, wherein the optical fiber has a first divergence angle θ′, the method comprising: arranging an array of glass microspheres adjacent the distal end, the microspheres having diameters in the range from 3 microns to 25 microns; and applying heat to the microspheres and to the distal end to fuse the microspheres to each other and to the distal end to form a light-redirecting structure that consists only of the microspheres and that defines a second divergence angle θ, wherein θ≥2θ′. 14 . The method according to claim 13 , further comprising: prior to the act of applying heat, using a binding material to bind the microspheres to each other and to the distal end of the optical fiber; and wherein the act of applying heat includes burning off substantially all of the binding material. 15 . The method according to claim 13 , wherein the optical fiber has a core diameter of nominally 40 microns and a cladding outer diameter of nominally 50 microns. 16 . The method according to claim 13 , wherein the glass microspheres, the core and the cladding are made of a silica-based glass. 17 . The method according to claim 13 , wherein the array has between 1 layer and 6 layers of microspheres. 18 . The method according to claim 13 , wherein the microspheres are solid. 19 . The method according to claim 13 , wherein the fused microspheres define air-filled interstices within the light-redirecting structure. 20 . The method according to claim 13 , wherein the core has a diameter D 1 and further including a widened core section adjacent the distal end, the widened core section having a diameter D 1 ′ that is at least 5% greater than the core diameter D 1 . 21 . The method according to claim 13 , further comprising forming the distal end of the optical fiber to have a convex curvature. 22 . The method according to claim 13 , further comprising forming protrusions and depressions on the distal end of the optical fiber, wherein the protrusion and depressions have a size substantially the same as the microspheres. 23 . The method according to claim 13 , further comprising: operably arranging a light source relative to the proximal end of the optical fiber and emitting a divergent light beam from the light-redirecting structure. 24 . The method according to claim 23 , wherein the light source emits non-polarized broadband visible light over a spectral range from 440 nm to 650 nm. 25 . The method according to claim 23 , further including immersing the light-redirecting structure in a biological fluid. 26 . A light source system that emits divergent light of a visible wavelength λ, comprising: a multimode optical fiber having a proximal end and a distal end, and a divergence angle θ′; a light-redirecting structure operably disposed at the distal end and consisting of an array of fused glass microspheres having diameters in the range from 3 microns to 25 microns, wherein the light-redirecting structure is fused to the distal end and has between 1 layer and 10 layers of microspheres and defines a divergence angle θ, wherein θ≥2θ′; and a light source optically coupled to the proximate end of the optical fiber and that emits light of the visible wavelength λ.
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