Saliva glucose measurement devices and methods

Patent metadata

Field	Value
Publication number	US-2019024131-A1
Application number	US-201716068877-A
Country	US
Kind code	A1
Filing date	Jan 27, 2017
Priority date	Jan 29, 2016
Publication date	Jan 24, 2019
Grant date	—

Abstract

Devices and methods capable of detecting glucose in saliva (FIG. 12). The devices feature a sensor having a substrate containing electrodes and one or more reagents on the electrodes. A detection device is operably coupled with the sensor to detect glucose based on measurement of an electrical parameter when electricity is applied to the electrode.

First claim

1 . A device configured to detect glucose in saliva, comprising:
a sensor having a substrate containing electrodes and one or more glucose-detection reagents on said electrodes; and a detection device operably coupled with said sensor to detect said glucose based on measurement of an electrical parameter when electricity is applied to said electrodes.

2 . The sensor device of claim 1 , wherein said one or more reagents comprise glucose dehydrogenase (GDH) and ferricyanide in 1×PBS buffer.

3 . The sensor device of claim 1 , further including a sulfonated tetrafluoroethylene-based fluoropolymer-copolymer coating or mesoporous carbon coating on one or more of the electrodes.

4 . The sensor device of claim 2 , wherein said reagents are in dry form.

5 . The sensor of claim 1 , wherein said one or more reagents comprise glucose dehydrogenase flavine-adenine dinucleotide (GDH-FAD) and ferricyanide in 1×PBS buffer.

6 . A method of detecting a blood glucose level using saliva, the method comprising:
contacting a sensor containing electrodes and one or more glucose-detection reagents on said electrodes with saliva; and energizing said electrodes such that a measurement of an electrical parameter indicative of an amount of glucose in said saliva is produced and measured.

7 . The method of claim 6 , wherein said one or more reagents comprise glucose dehydrogenase (GDH) and ferricyanide in 1×PBS buffer.

8 . The method of claim 6 , wherein said one or more reagents comprise glucose dehydrogenase flavine-adenine dinucleotide (GDH-FAD) and ferricyanide in 1×PBS buffer.

9 . A method of monitoring a glucose level against a desired range in a patient, comprising:
contacting a sensor containing electrodes and one or more glucose-detection reagents on said electrodes with saliva; and energizing said electrodes such that a measurement of an electrical parameter indicative of an amount of glucose in said saliva is produced and measured against said desired range.

10 . The method of claim 9 , wherein said one or more glucose-detection reagents comprise glucose dehydrogenase (GDH) and ferricyanide in 1×PBS buffer.

11 . The method of claim 9 , further including a sulfonated tetrafluoroethylene-based fluoropolymer-copolymer coating or mesoporous carbon coating on one or more of the electrodes.

12 . The method of claim 10 , wherein said glucose-detections reagents are in dry form.

13 . The method of claim 9 , wherein said one or more reagents comprise glucose dehydrogenase flavine-adenine dinucleotide (GDH-FAD) and ferricyanide in 1×PBS buffer.

14 . The method of claim 6 , wherein said glucose-detections reagents are in dry form.

Assignees

• Univ Arizona State

Inventors

• Labelle Jeffrey
• Lin Chi
• Reyna Jonus
• Alkhan Amnah
• Muggeridge Cael
• Sheffield Susan

Classifications

• G01N27/3273

  Devices therefor, e.g. test element readers, circuitry (details not specific to biochemical electrodes G01N33/4875) · CPC title

• G01N27/3272

  Test elements therefor, i.e. disposable laminated substrates with electrodes, reagent and channels (optical biosensors G01N33/52) · CPC title

• C12Q1/006 Primary

  for glucose · CPC title

• G01N27/31

  Half-cells with permeable membranes, e.g. semi-porous or perm-selective membranes · CPC title

• G01N27/70

  and measuring current or voltage · CPC title