Bacillus bombysepticus sf3 decomposing fluorine-containing compound, recombinant microorganism including gene derived from bacillus bombysepticus and method of reducing concentration of fluorine-containing compound in sample by using bacillus bombysepticus

Patent metadata

Field	Value
Publication number	US-2019024092-A1
Application number	US-201816044237-A
Country	US
Kind code	A1
Filing date	Jul 24, 2018
Priority date	Jul 24, 2017
Publication date	Jan 24, 2019
Grant date	—

Abstract

Provided are a microorganism having activity in reducing a concentration of a fluorine-containing compound in a sample, a recombinant microorganism including a gene derived from the microorganism, and a method of reducing the concentration of the fluorine-containing compound in the sample by using the microorganism or recombinant microorganism.

First claim

What is claimed is:

1 . A microorganism deposited with the Korean Collection for Type Culture (KCTC) under accession no. 13220BP and referred to as Bacillus bombysepticus SF3, which microorganism when contacted with a sample containing a fluorine-containing compound of Formula 1 or 2 reduces the concentration of the fluorine containing compound in the sample:
C(R 1 )(R 2 )(R 3 )(R 4 )  <Formula 1>
(R 5 )(R 6 )(R 7 )C—[C(R 11 )(R 12 )] n -C(R 8 )(R 9 )(R 10 )  <Formula 2>
wherein, in Formulae 1 and 2, n is an integer from 0 to 10, R 1 , R 2 , R 3 , and R 4 are each independently fluorine (F), chlorine (Cl), bromine (Br), iodine (I), or hydrogen (H), wherein at least one selected from R 1 , R 2 , R 3 , and R 4 is F, and R 5 , R 6 , R 7 , R 8 , R 9 , R 10 , R 11 , and R 12 are each independently F, Cl, Br, I, or H, wherein at least one selected from R 5 , R 6 , R 7 , R 8 , R 9 , R 10 , R 11 , and R 12 is F, and wherein, when n is equal or larger than 2, each R 11 is identical to or different from each other, and each R 12 is identical or different from each other.

2 . A recombinant microorganism comprising a genetic modification that increases the level of a polypeptide or a combination thereof, the polypeptide having a sequence identity of about 90% or more with respect to an amino acid sequence of SEQ ID NO: 1, 2, or 3.

3 . The recombinant microorganism of claim 2 , wherein the genetic modification is an increase in copy number of a gene encoding the polypeptide.

4 . The recombinant microorganism of claim 2 , wherein the recombinant microorganism comprises a exogenous gene encoding the polypeptide.

5 . The recombinant microorganism of claim 3 , wherein the gene has a sequence identity of about 90% or more with respect to a nucleotide sequence of SEQ ID NO: 4, 5, or 6.

6 . The recombinant microorganism of claim 2 , wherein the recombinant microorganism belongs to the genus Escherichia, Bacillus, Pseudomonas, Xanthobacter , or Saccharomyces.

7 . A composition comprising
(a) Bacillus bombysepticus SF3 (KCTC 13220BP) or a recombinant microorganism comprising a genetic modification that increases a level of a polypeptide that has a sequence identity of about 90% or more with respect to an amino acid sequence of SEQ ID NO: 1, 2, or 3; or a combination thereof; and (b) a fluorine-containing compound of Formula 1 or Formula 2:
C(R 1 )(R 2 )(R 3 )(R 4 )  <Formula 1>

(R 5 )(R 6 )(R 7 )C—[C(R 11 )(R 12 )] n -C(R 8 )(R 9 )(R 10 )  <Formula 2>

wherein, in Formulae 1 and 2, n is an integer from 0 to 10; R 1 , R 2 , R 3 , and R 4 are each independently fluorine (F), chlorine (Cl), bromine (Br), iodine (I), or hydrogen (H), wherein at least one of R 1 , R 2 , R 3 , or R 4 is F; and R 5 , R 6 , R 7 , R 8 , R 9 , R 10 , R 11 , and R 12 are each independently F, Cl, Br, I, or H, wherein at least one of R 5 , R 6 , R 7 , R 8 , R 9 , R 10 , R 11 , or R 12 is F; and wherein, when n is equal or larger than 2, each R 11 is identical to or different from each other, and each R 12 is identical to or different from each other.

8 . The composition of claim 7 , wherein the genetic modification is an increase in copy number of a gene encoding the polypeptide.

9 . The composition of claim 7 , wherein the Bacillus bombysepticus SF3 (KCTC 13220BP) or recombinant microorganism comprising a genetic modification that increases a level of a polypeptide that has a sequence identity of about 90% or more with respect to an amino acid sequence of SEQ ID NO: 1, 2, or 3, or both, when in contact with a sample containing a fluorine-containing compound of Formula 1 or Formula 2 has the ability to reduce the concentration of the fluorine compound in the sample, optionally, by cleaving a C—F bond of the fluorine-containing compound, converting the fluorine-containing compound into a different substance, or accumulating the fluorine-containing compound in the microorganism.

10 . The composition of claim 7 , wherein the fluorine-containing compound of Formula 1 or Formula 2 is in a liquid or gaseous state.

11 . The composition of claim 7 , wherein the recombinant microorganism belongs to the genus Escherichia, Bacillus, Pseudomonas, Xanthobacter , or Saccharomyces.

12 . A method of reducing a concentration of a fluorine-containing compound in a sample, the method comprising:
contacting a sample comprising a fluorine-containing compound with Bacillus bombysepticus SF3 (KCTC 13220BP) or a recombinant microorganism comprising a genetic modification that increases a level of a polypeptide that has a sequence identity of about 90% or more with respect to the amino acid sequence of SEQ ID NO: 1, 2, or 3, or a combination thereof, so as to reduce the concentration of the fluorine-containing compound in the sample, wherein the fluorine-containing compound is represented by Formula 1 or Formula 2:
C(R 1 )(R 2 )(R 3 )(R 4 )  <Formula 1>

(R 5 )(R 6 )(R 7 )C—[C(R 11 )(R 12 )] n -C(R 8 )(R 9 )(R 10 )  <Formula 2>

wherein, in Formulae 1 and 2, n is an integer from 0 to 10; R 1 , R 2 , R 3 , and R 4 are each independently fluorine (F), chlorine (Cl), bromine (Br), iodine (I), or hydrogen (H), wherein at least one of R 1 , R 2 , R 3 , or R 4 is F; and R 5 , R 6 , R 7 , R 8 , R 9 , R 10 , R 11 , and R 12 are each independently F, Cl, Br, I, or H, wherein at least one of R 5 , R 6 , R 7 , R 8 , R 9 , R 10 , R 11 , or R 12 is F; and wherein, when n is equal or larger than 2, each R 11 is identical to or different from each other, and each R 12 is identical to or different from each other.

13 . The method of claim 12 , wherein the genetic modification is an increase in copy number of a gene encoding the polypeptide.

14 . The method of claim 12 , wherein the contacting is performed in an air-tight sealed container.

15 . The method of claim 12 , wherein the contacting comprises culturing or incubating B. bombysepticus SF3 (KCTC 13220BP) or the recombinant microorganism while in contact with the sample.

16 . The method of claim 12 , wherein the contacting comprises culturing B. bombysepticus SF3 (KCTC 13220BP) or the recombinant microorganism under conditions in which B. bombysepticus SF3 (KCTC 13220BP) or the recombinant microorganism proliferates in a closed container.

17 . The method of claim 12 , wherein the contacting comprises, in an exhaust gas decomposition device comprising one or more reactors each of which comprises at least one first inlet and a first outlet:
injecting the sample into the exhaust gas decomposition device; and injecting B. bombysepticus SF3 (KCTC 13220BP) or the recombinant microorganism through the at least one first inlet so that B. bombysepticus SF3 (KCTC 13220BP) or the recombinant microorganism contacts the sample and the resulting mixture is discharged through the first outlet.

18 . The method of claim 17 , wherein the exhaust gas decomposition device comprises a second inlet and a second outlet, the sample is injected through the second inlet and discharged through the second outlet, and a direction in which B. bombysepticus SF3 (KCTC 13220BP) or the recombinant microorganism moves is opposite to a direction in which the sample moves.

19 . The method of claim 17 , wherein a fluid thin film comprising B. bombysepticus SF3 (KCTC 13220BP) or the recombinant microorganism is formed on an inner wall of the one or more reactors, or on a packing material when the one or more reactors comprises a packing material.

20 . A vect

Assignees

• Samsung Electronics Co Ltd

Inventors

• Jung Yukyung
• Byun Jongwon
• Kim Taeyong
• Park Jinhwan
• Song Seunghoon

Classifications

• B01D53/70

  Organic halogen compounds · CPC title

• C02F3/02

  Aerobic processes · CPC title

• B01D2257/2066

  Fluorine · CPC title

• C12Y308/01005

  Haloalkane dehalogenase (3.8.1.5) · CPC title

• B01D2255/804

  Enzymatic · CPC title