System for hydrogen production under limited aerobic conditions

Patent metadata

Field	Value
Publication number	US-2019002924-A1
Application number	US-201815912385-A
Country	US
Kind code	A1
Filing date	Mar 5, 2018
Priority date	Jun 29, 2017
Publication date	Jan 3, 2019
Grant date	—

Abstract

The present invention provides a method for fermentative hydrogen production under limited aerobic conditions by utilizing the respiratory interaction between a strictly anaerobic hydrogen producing bacterium, E. harbinense YUAN-3, and a facultative anaerobic bacterium, P. aeruginosa PAO1. The two bacteria are co-cultured to produce hydrogen gas in a culture medium without any anaerobic treatment. Sucrose, lactose or glucose are used as the carbon source for the co-culture which can promote the growth of E. harbinense YUAN-3 and reduce substrate competition between two bacteria. L-cysteine is added to increase the hydrogen yield and the production rate. Using 15 g/L glucose and 5 mmol/L L-cysteine, the invented method achieved the hydrogen production yield of 1.11 mol-hydrogen/mol-glucose.

First claim

1 . A method for hydrogen production under limited aerobic conditions, comprising:
a, co-culturing E. harbinense YUAN-3 and P. aeruginosa PAO1 in a liquid medium containing 10 to 20 g/L sucrose or lactose in a sealed container at 35° C.; and b, collecting hydrogen gas at 1 to 50 hours after the start of the co-culture.

2 . (canceled)

3 . The method of claim 1 , wherein the liquid medium contains 15 g/L sucrose or lactose.

4 . The method of claim 1 , wherein the liquid medium further comprises 0 to 15 mmol/L of L-cysteine.

5 . The method of claim 1 , wherein the liquid medium further comprises 5 to 10 mmol/L of L-cysteine.

6 . The method of claim 1 , wherein the liquid medium comprises 5 mmol/L of L-cysteine.

7 . The method of claim 1 , wherein the liquid medium comprises 4 g peptone, 1 g yeast extract, 2 g beef extract, 4 g NaCl, 1.0 g K 2 HPO 4 and 0.2 g MgCl 2 .6H 2 O per liter.

8 . The method of claim 1 , wherein 0.1-0.2 g of E. harbinense YUAN-3 and 0.08-0.12 g of P. aeruginosa PAO1 are inoculated into the liquid medium.

9 . The method of claim 1 , wherein during the sealed culture, a shaker or a magnetic stirrer is used for agitation and culture; wherein conditions for culturing using the shaker are: 35° C., rotation speed 170 rpm; and conditions for culturing using the magnetic stirrer are: 35° C., 3 cm magnetic stirrer, stirring speed of 70 rpm.

Assignees

• Xing Defeng
• Yu Najiaowa
• Li Wei
• Li Zhen
• Yang Yang
• Harbin Inst Technology

Inventors

• Xing Defeng
• Yu Najiaowa
• Li Wei
• Li Zhen
• Yang Yang

Classifications

• C12R1/145

  Chemistry & Metallurgy · mapped topic

• C07H3/02

  Monosaccharides · CPC title

• C07C323/58

  with amino groups bound to the carbon skeleton · CPC title

• C07H3/04

  Disaccharides · CPC title

• C12N1/20

  Bacteria; Culture media therefor · CPC title