Isolated polynucleotides, polypeptides and methods of using same for increasing abiotic stress tolerance, biomass and yield of plants

Patent metadata

Field	Value
Publication number	US-2018362996-A1
Application number	US-201816102903-A
Country	US
Kind code	A1
Filing date	Aug 14, 2018
Priority date	May 28, 2014
Publication date	Dec 20, 2018
Grant date	—

Abstract

Provided are isolated polypeptides which are at least 80% homologous to SEQ ID NOs: 552-897 and 6029-10629, isolated polynucleotides which are at least 80% identical to SEQ ID NOs: 1-551 and 898-6028, nucleic acid constructs comprising same, transgenic cells expressing same, transgenic plants expressing same and method of using same for increasing yield, abiotic stress tolerance, growth rate, biomass, vigor, oil content, photosynthetic capacity, seed yield, fiber yield, fiber quality, fiber length, and/or nitrogen use efficiency of a plant.

First claim

What is claimed is:

1 . A method of increasing yield, growth rate, biomass, vigor, oil content, seed yield, fiber yield, fiber quality, fiber length, photosynthetic capacity, nitrogen use efficiency, and/or abiotic stress tolerance of a plant and/or reducing time to flowering and/or time to inflorescence emergence of a plant, the method comprising transforming a cell of the plant with a nucleic acid construct comprising a polynucleotide which comprises a nucleic acid sequence encoding a polypeptide comprising an amino acid sequence at least 80% identical to SEQ ID NO: 623, 552-622, 624-633, 635-725, 727-773, 775-780, 782-786, 789-885, 887-889, 891-897, 6029-7467, 7481, 7487, 7498-7499, 7501-7503, 7512-7513, 7515, 7517, 7522, 7525, 7529, 7533-7534, 7539-7541, 7545, 7549, 7552, 7555-7556, 7558, 7563, 7576, 7579, 7588, 7590, 7592-7593, 7595, 7609-7612, 7614-7615, 7620, 7624, 7627, 7631, 7633, 7637, 7639, 7643-7644, 7647, 7649, 7651, 7653-7658, 7660, 7662, 7664, 7666, 7672-7673, 7677-7678, 7680-7681, 7683-7684, 7688-7690, 7692, 7694, 7699-7703, 7705-7706, 7709-7711, 7716-7719, 7721-7723, 7726-7732, 7736-7738, 7740-7742, 7745, 7747-7748, 7751, 7758, 7760-7762, 7765-7766, 7769, 7773, 7777-7781, 7783-7785, 7787-7789, 7791, 7795-7800, 7802-7811, 7813, 7815-8160, 8162, 8164-8853, 8855-9215, 9238-9749, 9751-9803, 9805-9818, 9828, 9935-9968, 9970-9971, 9973-10187, 10189, 10191-10585, 10600-10605, 10609-10628 or 10629, and a promoter for directing transcription of said nucleic acid sequence in said plant cell, wherein said promoter is heterologous to said polynucleotide, thereby increasing the yield, growth rate, biomass, vigor, oil content, seed yield, fiber yield, fiber quality, fiber length, photosynthetic capacity, nitrogen use efficiency, and/or abiotic stress tolerance of the plant and/or reducing the time to flowering and/or the time to inflorescence emergence of the plant.

2 . The method of claim 1 , wherein said polypeptide is selected from the group consisting of SEQ ID NOs: 623, 7276-7293, 552-622, 624-773, 775-780, 782-786, 789-885, 887-897, 6029-7275, 7294-7781, 7783-9818, 9820-9823, 9827-9828, 9840-9841, 9849, 9852-9854, 9856, 9858-9859, 9867, 9870, 9872, 9874-9875, 9881, 9883-9885, 9887, 9891, 9893, 9896, 9898-9902, 9904, 9906-9908, 9911, 9915, 9917, 9919, 9921-9922, 9924-9926, 9929, 9933-10585, 10589, 10593, 10599-10605, 10607-10628 and 10629.

3 . The method of claim 1 , wherein said nucleic acid sequence is at least 80% identical to SEQ ID NOs: 1-82, 84-174, 176-222, 224-229, 231-235, 238-302, 304-387, 389-473, 475-519, 521-526, 528-532, 535-551, 898-2468, 2485, 2492-2493, 2495, 2507-2508, 2510-2512, 2523-2524, 2526, 2528, 2533, 2537, 2541, 2545-2546, 2551-2553, 2557, 2564, 2567, 2573-2574, 2576-2577, 2583, 2594, 2599, 2602, 2611, 2613-2614, 2616-2617, 2619, 2635-2638, 2640-2642, 2648, 2652, 2655, 2660, 2662, 2666, 2668, 2673-2674, 2677, 2679, 2681, 2683-2688, 2691, 2693, 2695-2698, 2700, 2707-2708, 2713-2714, 2716-2717, 2719-2720, 2724-2726, 2728, 2730-2731, 2736-2742, 2744-2746, 2751-2753, 2757, 2759-2762, 2764-2766, 2769-2776, 2780-2783, 2785-2788, 2791, 2793-2795, 2798, 2805, 2807-2808, 2812, 2814-2815, 2818-2820, 2823, 2829, 2834-2838, 2840-2842, 2844-2846, 2848, 2852-2858, 2860-2872, 2874, 2876-3244, 3246, 3248-4015, 4017-4426, 4449-5012, 5015-5071, 5073-5090, 5101, 5255, 5267-5304, 5306-5307, 5309-5539, 5541, 5543-5976, 5994-5999, 6003-6027 or 6028.

4 . The method of claim 1 , further comprising selecting a plant transformed with said nucleic acid construct for a reduced time to flowering and/or for a reduced time to inflorescence emergence as compared to a wild type plant of the same species which is grown under the same growth conditions.

5 . The method of claim 1 , further comprising selecting a plant transformed with said nucleic acid construct for an increased yield, growth rate, biomass, vigor, oil content, seed yield, fiber yield, fiber quality, fiber length, photosynthetic capacity, nitrogen use efficiency, and/or abiotic stress tolerance as compared to the wild type plant of the same species which is grown under the same growth conditions.

6 . The method of claim 4 , wherein said selecting is performed under non-stress conditions.

7 . The method of claim 4 , wherein said selecting is performed under abiotic stress conditions.

8 . The method of claim 1 , further comprising growing the plant transformed with said nucleic acid construct under the abiotic stress.

9 . The method of claim 1 , wherein said abiotic stress is selected from the group consisting of drought, salinity, osmotic stress, water deprivation, flood, etiolation, low temperature, high temperature, heavy metal toxicity, anaerobiosis, nutrient deficiency, nitrogen deficiency, nutrient excess, atmospheric pollution and UV irradiation.

10 . The method of claim 1 , further comprising growing the plant transformed with said nucleic acid construct under nitrogen-limiting conditions.

11 . A method of producing a crop, the method comprising growing a crop plant transformed with a nucleic acid construct comprising a polynucleotide which comprises a nucleic acid sequence encoding a polypeptide comprising an amino acid sequence at least 80% identical to the amino acid sequence selected from the group consisting of SEQ ID NOs: 623, 552-622, 624-633, 635-725, 727-773, 775-780, 782-786, 789-885, 887-889, 891-897, 6029-7467, 7481, 7487, 7498-7499, 7501-7503, 7512-7513, 7515, 7517, 7522, 7525, 7529, 7533-7534, 7539-7541, 7545, 7549, 7552, 7555-7556, 7558, 7563, 7576, 7579, 7588, 7590, 7592-7593, 7595, 7609-7612, 7614-7615, 7620, 7624, 7627, 7631, 7633, 7637, 7639, 7643-7644, 7647, 7649, 7651, 7653-7658, 7660, 7662, 7664, 7666, 7672-7673, 7677-7678, 7680-7681, 7683-7684, 7688-7690, 7692, 7694, 7699-7703, 7705-7706, 7709-7711, 7716-7719, 7721-7723, 7726-7732, 7736-7738, 7740-7742, 7745, 7747-7748, 7751, 7758, 7760-7762, 7765-7766, 7769, 7773, 7777-7781, 7783-7785, 7787-7789, 7791, 7795-7800, 7802-7811, 7813, 7815-8160, 8162, 8164-8853, 8855-9215, 9238-9749, 9751-9803, 9805-9818, 9828, 9935-9968, 9970-9971, 9973-10187, 10189, 10191-10585, 10600-10605, 10609-10628 or 10629, and a promoter for directing transcription of said nucleic acid sequence in said plant cell, wherein said promoter is heterologous to said polynucleotide, wherein the crop plant is derived from plants which comprise said nucleic acid construct and which have been selected for a reduced time to flowering, a reduced time to inflorescence emergence, an increased yield, an increased growth rate, an increased biomass, an increased vigor, an increased oil content, an increased seed yield, an increased fiber yield, an increased fiber quality, an increased fiber length, an increased photosynthetic capacity, an increased nitrogen use efficiency, and/or an increased abiotic stress tolerance as compared to a wild type plant of the same species which is grown under the same growth conditions, and the crop plant having the reduced time to flowering, the reduced time to inflorescence emergence, the increased yield, the increased growth rate, the increased biomass, the increased vigor, the increased oil content, the increased seed yield, the increased fiber yield, the increased fiber quality, the increased fiber length, the increased photosynthetic capacity, the increased nitrogen use efficiency, and/or the increased abiotic stress tolerance, thereby producing the crop.

12 . The method of claim 11 , wherein said amino acid sequence is selected from the group consisting of SEQ ID NOs: 623, 7276-7293, 552-622, 624-773, 775-780, 782-786, 789-885, 887-897, 6029-7275, 7294-7781, 7783-9818, 9820-9823, 9827-9828, 9840-9841, 9849, 9852-9854, 9856, 9858-9859, 9867, 9870, 9872,

Assignees

• Evogene Ltd

Inventors

• Dangoor Inbal Nurith
• Ofir-Birin Yifat Louba
• Brog Yaacov Micha
• Vinocur Basia Judith
• Karchi Hagai

Classifications

• C12N15/8261

  with agronomic (input) traits, e.g. crop yield · CPC title

• C12N15/8271 Primary

  for stress resistance, e.g. heavy metal resistance · CPC title

• C07K14/415 Primary

  from plants · CPC title

• C12N15/8247

  involving modified lipid metabolism, e.g. seed oil composition · CPC title

• C12N15/8273

  for drought, cold, salt resistance · CPC title