Universally dispersible particles containing hydrogen bonds
US-2015353650-A1 · Dec 10, 2015 · US
US2018355120A1 · US · A1
| Field | Value |
|---|---|
| Publication number | US-2018355120-A1 |
| Application number | US-201615780477-A |
| Country | US |
| Kind code | A1 |
| Filing date | Nov 29, 2016 |
| Priority date | Dec 1, 2015 |
| Publication date | Dec 13, 2018 |
| Grant date | — |
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Provided is a method for producing a protein solution, including applying a pressure to a dispersion liquid containing a protein and a polar solvent in which the protein is dispersed, to obtain a protein solution containing the protein and the polar solvent in which the protein is dissolved.
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1 . A method for producing a protein solution, comprising: applying a pressure to a dispersion liquid containing a protein and a polar solvent in which the protein is dispersed, to obtain a protein solution containing the protein and the polar solvent in which the protein is dissolved. 2 . The method according to claim 1 , wherein the pressure is 0.05 MPa or more. 3 . The method according to claim 1 , wherein the protein is a structural protein. 4 . The method according to claim 3 , wherein the structural protein is one or more structural proteins selected from the group consisting of fibroin and keratin. 5 . The method according to claim 3 , wherein the structural protein is one or more fibroins selected from the group consisting of silk fibroin, spider silk fibroin, and hornet silk fibroin. 6 . The method according to claim 1 , wherein the pressure is applied to the dispersion liquid while the dispersion liquid is being heated. 7 . The method according to claim 1 , wherein the polar solvent includes one or more solvents selected from the group consisting of water, alcohol, dimethylsulfoxide, dimethylformamide, and hexafluoroacetone. 8 . A protein solution comprising a spider silk fibroin and a polar solvent in which the spider silk fibroin is dissolved, wherein a concentration of the spider silk fibroin dissolved in the polar solvent is 1% to 70% by mass based on a mass of the protein solution. 9 . A method for producing a molded article, comprising: obtaining a protein solution containing a protein and a polar solvent in which the protein is dissolved, by the method according to claim 1 ; and removing the polar solvent from the protein solution to obtain a molded article containing the protein. 10 . A method for regenerating a protein from a molded article, comprising: applying a pressure to a polar solvent, which is in contact with the molded article containing the protein, to obtain a protein solution containing the protein and the polar solvent in which the protein is dissolved; and collecting the protein from the protein solution. 11 . The method according to claim 10 , wherein the pressure is 0.05 MPa or more. 12 . The method according to claim 10 , wherein the protein is a structural protein. 13 . The method according to claim 12 , wherein the structural protein is one or more structural proteins selected from the group consisting of fibroin and keratin. 14 . The method according to claim 12 , wherein the structural protein is one or more fibroins selected from the group consisting of silk fibroin, spider silk fibroin, and hornet silk fibroin. 15 . The method according to claim 10 , wherein the pressure is applied to the polar solvent while the polar solvent is being heated. 16 . The method according to claim 10 , wherein the polar solvent includes one or more solvents selected from the group consisting of water, alcohol, dimethylsulfoxide, dimethylformamide, and hexafluoroacetone.
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