Method for Producing Protein Solution

US2018355120A1 · US · A1

Patent metadata
FieldValue
Publication numberUS-2018355120-A1
Application numberUS-201615780477-A
CountryUS
Kind codeA1
Filing dateNov 29, 2016
Priority dateDec 1, 2015
Publication dateDec 13, 2018
Grant date

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Abstract

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Provided is a method for producing a protein solution, including applying a pressure to a dispersion liquid containing a protein and a polar solvent in which the protein is dispersed, to obtain a protein solution containing the protein and the polar solvent in which the protein is dissolved.

First claim

Opening claim text (preview).

1 . A method for producing a protein solution, comprising: applying a pressure to a dispersion liquid containing a protein and a polar solvent in which the protein is dispersed, to obtain a protein solution containing the protein and the polar solvent in which the protein is dissolved. 2 . The method according to claim 1 , wherein the pressure is 0.05 MPa or more. 3 . The method according to claim 1 , wherein the protein is a structural protein. 4 . The method according to claim 3 , wherein the structural protein is one or more structural proteins selected from the group consisting of fibroin and keratin. 5 . The method according to claim 3 , wherein the structural protein is one or more fibroins selected from the group consisting of silk fibroin, spider silk fibroin, and hornet silk fibroin. 6 . The method according to claim 1 , wherein the pressure is applied to the dispersion liquid while the dispersion liquid is being heated. 7 . The method according to claim 1 , wherein the polar solvent includes one or more solvents selected from the group consisting of water, alcohol, dimethylsulfoxide, dimethylformamide, and hexafluoroacetone. 8 . A protein solution comprising a spider silk fibroin and a polar solvent in which the spider silk fibroin is dissolved, wherein a concentration of the spider silk fibroin dissolved in the polar solvent is 1% to 70% by mass based on a mass of the protein solution. 9 . A method for producing a molded article, comprising: obtaining a protein solution containing a protein and a polar solvent in which the protein is dissolved, by the method according to claim 1 ; and removing the polar solvent from the protein solution to obtain a molded article containing the protein. 10 . A method for regenerating a protein from a molded article, comprising: applying a pressure to a polar solvent, which is in contact with the molded article containing the protein, to obtain a protein solution containing the protein and the polar solvent in which the protein is dissolved; and collecting the protein from the protein solution. 11 . The method according to claim 10 , wherein the pressure is 0.05 MPa or more. 12 . The method according to claim 10 , wherein the protein is a structural protein. 13 . The method according to claim 12 , wherein the structural protein is one or more structural proteins selected from the group consisting of fibroin and keratin. 14 . The method according to claim 12 , wherein the structural protein is one or more fibroins selected from the group consisting of silk fibroin, spider silk fibroin, and hornet silk fibroin. 15 . The method according to claim 10 , wherein the pressure is applied to the polar solvent while the polar solvent is being heated. 16 . The method according to claim 10 , wherein the polar solvent includes one or more solvents selected from the group consisting of water, alcohol, dimethylsulfoxide, dimethylformamide, and hexafluoroacetone.

Assignees

Inventors

Classifications

  • C08J3/02Primary

    Making solutions, dispersions, lattices or gels by other methods than by solution, emulsion or suspension polymerisation techniques · CPC title

  • from silkworms · CPC title

  • Extraction; Separation; Purification · CPC title

  • Characterised by the use of proteins; Derivatives thereof · CPC title

  • C08L89/00Primary

    Compositions of proteins; Compositions of derivatives thereof (foodstuff preparations A23J3/00) · CPC title

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What does patent US2018355120A1 cover?
Provided is a method for producing a protein solution, including applying a pressure to a dispersion liquid containing a protein and a polar solvent in which the protein is dispersed, to obtain a protein solution containing the protein and the polar solvent in which the protein is dissolved.
Who is the assignee on this patent?
Spiber Inc, Kojima Ind Corp, Tekunohama Co Ltd
What technology area does this patent fall under?
Primary CPC classification C08J3/02. Mapped technology areas include Chemistry & Metallurgy.
When was this patent published?
Publication date Thu Dec 13 2018 00:00:00 GMT+0000 (Coordinated Universal Time) (A1). Legal status and post-grant events are not shown on this page.
What related patents are in patentsdb?
We list 8 related publications on this page (citations in our corpus or others sharing the same primary CPC).